Experiment: D-Raffinose 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS08070 and HMPREF1058_RS08065 are separated by 8 nucleotides HMPREF1058_RS08065 and HMPREF1058_RS08060 are separated by 51 nucleotides HMPREF1058_RS08060 and HMPREF1058_RS08055 are separated by 62 nucleotides HMPREF1058_RS08055 and HMPREF1058_RS08050 are separated by 16 nucleotides
HMPREF1058_RS08070: HMPREF1058_RS08070 - alpha-L-fucosidase, at 60,905 to 62,944
_RS08070
HMPREF1058_RS08065: HMPREF1058_RS08065 - tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA, at 62,953 to 63,855
_RS08065
HMPREF1058_RS08060: HMPREF1058_RS08060 - hypothetical protein, at 63,907 to 64,470
_RS08060
HMPREF1058_RS08055: HMPREF1058_RS08055 - acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase, at 64,533 to 65,300
_RS08055
HMPREF1058_RS08050: HMPREF1058_RS08050 - bifunctional UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP dehydratase, at 65,317 to 66,702
_RS08050
Position (kb) 63
64
65 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 62.907 kb on - strand at 62.931 kb on - strand at 62.931 kb on - strand at 62.934 kb on - strand at 62.973 kb on + strand at 62.974 kb on - strand at 63.123 kb on - strand, within HMPREF1058_RS08065 at 63.140 kb on + strand, within HMPREF1058_RS08065 at 63.181 kb on + strand, within HMPREF1058_RS08065 at 63.182 kb on - strand, within HMPREF1058_RS08065 at 63.182 kb on - strand, within HMPREF1058_RS08065 at 63.182 kb on - strand, within HMPREF1058_RS08065 at 63.249 kb on - strand, within HMPREF1058_RS08065 at 63.350 kb on + strand, within HMPREF1058_RS08065 at 63.350 kb on + strand, within HMPREF1058_RS08065 at 63.350 kb on + strand, within HMPREF1058_RS08065 at 63.356 kb on - strand, within HMPREF1058_RS08065 at 63.370 kb on + strand, within HMPREF1058_RS08065 at 63.370 kb on + strand, within HMPREF1058_RS08065 at 63.371 kb on - strand, within HMPREF1058_RS08065 at 63.520 kb on + strand, within HMPREF1058_RS08065 at 63.541 kb on + strand, within HMPREF1058_RS08065 at 63.541 kb on + strand, within HMPREF1058_RS08065 at 63.541 kb on + strand, within HMPREF1058_RS08065 at 63.542 kb on - strand, within HMPREF1058_RS08065 at 63.542 kb on - strand, within HMPREF1058_RS08065 at 63.542 kb on - strand, within HMPREF1058_RS08065 at 63.543 kb on + strand, within HMPREF1058_RS08065 at 63.545 kb on + strand, within HMPREF1058_RS08065 at 63.545 kb on + strand, within HMPREF1058_RS08065 at 63.546 kb on - strand, within HMPREF1058_RS08065 at 63.546 kb on - strand, within HMPREF1058_RS08065 at 63.546 kb on - strand, within HMPREF1058_RS08065 at 63.546 kb on - strand, within HMPREF1058_RS08065 at 63.568 kb on + strand, within HMPREF1058_RS08065 at 63.571 kb on + strand, within HMPREF1058_RS08065 at 63.572 kb on - strand, within HMPREF1058_RS08065 at 63.613 kb on + strand, within HMPREF1058_RS08065 at 63.626 kb on + strand, within HMPREF1058_RS08065 at 63.674 kb on + strand, within HMPREF1058_RS08065 at 63.722 kb on - strand, within HMPREF1058_RS08065 at 63.799 kb on + strand at 63.814 kb on + strand at 63.815 kb on - strand at 63.887 kb on - strand at 63.908 kb on + strand at 63.927 kb on - strand at 64.018 kb on + strand, within HMPREF1058_RS08060 at 64.019 kb on - strand, within HMPREF1058_RS08060 at 64.188 kb on - strand, within HMPREF1058_RS08060 at 64.248 kb on - strand, within HMPREF1058_RS08060 at 64.248 kb on - strand, within HMPREF1058_RS08060 at 64.350 kb on + strand, within HMPREF1058_RS08060 at 64.350 kb on + strand, within HMPREF1058_RS08060 at 64.350 kb on + strand, within HMPREF1058_RS08060 at 64.351 kb on - strand, within HMPREF1058_RS08060 at 64.426 kb on - strand at 64.455 kb on + strand at 64.471 kb on - strand at 64.473 kb on - strand at 64.476 kb on + strand at 64.483 kb on + strand at 64.484 kb on - strand at 64.484 kb on - strand at 64.485 kb on + strand at 64.486 kb on - strand at 64.488 kb on - strand at 64.491 kb on - strand at 64.535 kb on - strand at 65.333 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Raffinose 2.5 mg/ml (C) remove 62,907 - +0.8 62,931 - -0.3 62,931 - -0.1 62,934 - +0.4 62,973 + +2.9 62,974 - -0.6 63,123 - HMPREF1058_RS08065 0.19 -2.3 63,140 + HMPREF1058_RS08065 0.21 -1.7 63,181 + HMPREF1058_RS08065 0.25 +0.2 63,182 - HMPREF1058_RS08065 0.25 -2.1 63,182 - HMPREF1058_RS08065 0.25 -2.0 63,182 - HMPREF1058_RS08065 0.25 -1.4 63,249 - HMPREF1058_RS08065 0.33 -0.6 63,350 + HMPREF1058_RS08065 0.44 -1.0 63,350 + HMPREF1058_RS08065 0.44 -1.7 63,350 + HMPREF1058_RS08065 0.44 -1.9 63,356 - HMPREF1058_RS08065 0.45 -1.1 63,370 + HMPREF1058_RS08065 0.46 +0.7 63,370 + HMPREF1058_RS08065 0.46 -0.3 63,371 - HMPREF1058_RS08065 0.46 -1.0 63,520 + HMPREF1058_RS08065 0.63 -0.8 63,541 + HMPREF1058_RS08065 0.65 -1.8 63,541 + HMPREF1058_RS08065 0.65 -0.7 63,541 + HMPREF1058_RS08065 0.65 -1.7 63,542 - HMPREF1058_RS08065 0.65 -0.5 63,542 - HMPREF1058_RS08065 0.65 -0.6 63,542 - HMPREF1058_RS08065 0.65 -0.3 63,543 + HMPREF1058_RS08065 0.65 -0.9 63,545 + HMPREF1058_RS08065 0.66 -1.9 63,545 + HMPREF1058_RS08065 0.66 -1.8 63,546 - HMPREF1058_RS08065 0.66 -2.3 63,546 - HMPREF1058_RS08065 0.66 -1.2 63,546 - HMPREF1058_RS08065 0.66 +0.7 63,546 - HMPREF1058_RS08065 0.66 -0.5 63,568 + HMPREF1058_RS08065 0.68 -1.0 63,571 + HMPREF1058_RS08065 0.68 -0.3 63,572 - HMPREF1058_RS08065 0.69 -0.3 63,613 + HMPREF1058_RS08065 0.73 -1.2 63,626 + HMPREF1058_RS08065 0.75 -1.0 63,674 + HMPREF1058_RS08065 0.80 -1.1 63,722 - HMPREF1058_RS08065 0.85 -0.4 63,799 + -0.0 63,814 + -0.5 63,815 - +1.5 63,887 - +0.1 63,908 + -1.0 63,927 - +0.4 64,018 + HMPREF1058_RS08060 0.20 -1.7 64,019 - HMPREF1058_RS08060 0.20 -1.5 64,188 - HMPREF1058_RS08060 0.50 -2.5 64,248 - HMPREF1058_RS08060 0.60 -1.0 64,248 - HMPREF1058_RS08060 0.60 -1.8 64,350 + HMPREF1058_RS08060 0.79 -2.1 64,350 + HMPREF1058_RS08060 0.79 -1.9 64,350 + HMPREF1058_RS08060 0.79 -2.1 64,351 - HMPREF1058_RS08060 0.79 -0.7 64,426 - -2.4 64,455 + -0.7 64,471 - +0.3 64,473 - -1.8 64,476 + -2.1 64,483 + +0.3 64,484 - +1.1 64,484 - +0.4 64,485 + +1.3 64,486 - +0.4 64,488 - -0.5 64,491 - -0.7 64,535 - -0.3 65,333 - -0.1
Or see this region's nucleotide sequence