Experiment: Red Arabinan from sugar-beet; filtered 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS06170 and HMPREF1058_RS06165 are separated by 41 nucleotides HMPREF1058_RS06165 and HMPREF1058_RS06160 overlap by 1 nucleotides HMPREF1058_RS06160 and HMPREF1058_RS06155 are separated by 264 nucleotides HMPREF1058_RS06155 and HMPREF1058_RS06150 are separated by 30 nucleotides HMPREF1058_RS06150 and HMPREF1058_RS06145 are separated by 29 nucleotides HMPREF1058_RS06145 and HMPREF1058_RS06140 are separated by 257 nucleotides
HMPREF1058_RS06170: HMPREF1058_RS06170 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 542,592 to 543,311
_RS06170
HMPREF1058_RS06165: HMPREF1058_RS06165 - imidazole glycerol phosphate synthase subunit HisH, at 543,353 to 543,943
_RS06165
HMPREF1058_RS06160: HMPREF1058_RS06160 - formyltetrahydrofolate deformylase, at 543,943 to 544,800
_RS06160
HMPREF1058_RS06155: HMPREF1058_RS06155 - tRNA-Asp, at 545,065 to 545,138
_RS06155
HMPREF1058_RS06150: HMPREF1058_RS06150 - tRNA-Asp, at 545,169 to 545,242
_RS06150
HMPREF1058_RS06145: HMPREF1058_RS06145 - tRNA-Asp, at 545,272 to 545,345
_RS06145
HMPREF1058_RS06140: HMPREF1058_RS06140 - phage integrase SAM-like domain-containing protein, at 545,603 to 546,238
_RS06140
Position (kb)
543
544
545 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 543.010 kb on - strand, within HMPREF1058_RS06170 at 543.228 kb on + strand, within HMPREF1058_RS06170 at 543.229 kb on - strand, within HMPREF1058_RS06170 at 543.244 kb on + strand at 543.300 kb on + strand at 543.301 kb on - strand at 543.301 kb on - strand at 543.315 kb on + strand at 543.316 kb on - strand at 543.328 kb on - strand at 543.328 kb on - strand at 543.335 kb on + strand at 543.340 kb on - strand at 543.340 kb on - strand at 543.342 kb on - strand at 543.467 kb on - strand, within HMPREF1058_RS06165 at 543.467 kb on - strand, within HMPREF1058_RS06165 at 543.507 kb on + strand, within HMPREF1058_RS06165 at 543.508 kb on - strand, within HMPREF1058_RS06165 at 543.519 kb on - strand, within HMPREF1058_RS06165 at 543.519 kb on - strand, within HMPREF1058_RS06165 at 543.638 kb on + strand, within HMPREF1058_RS06165 at 543.638 kb on + strand, within HMPREF1058_RS06165 at 543.722 kb on + strand, within HMPREF1058_RS06165 at 543.726 kb on - strand, within HMPREF1058_RS06165 at 543.737 kb on + strand, within HMPREF1058_RS06165 at 543.737 kb on + strand, within HMPREF1058_RS06165 at 543.746 kb on - strand, within HMPREF1058_RS06165 at 543.839 kb on + strand, within HMPREF1058_RS06165 at 543.840 kb on - strand, within HMPREF1058_RS06165 at 543.903 kb on + strand at 543.954 kb on + strand at 543.955 kb on - strand at 543.972 kb on - strand at 543.993 kb on + strand at 543.993 kb on + strand at 544.048 kb on + strand, within HMPREF1058_RS06160 at 544.087 kb on + strand, within HMPREF1058_RS06160 at 544.088 kb on - strand, within HMPREF1058_RS06160 at 544.225 kb on + strand, within HMPREF1058_RS06160 at 544.282 kb on - strand, within HMPREF1058_RS06160 at 544.431 kb on + strand, within HMPREF1058_RS06160 at 544.435 kb on + strand, within HMPREF1058_RS06160 at 544.435 kb on + strand, within HMPREF1058_RS06160 at 544.488 kb on + strand, within HMPREF1058_RS06160 at 544.541 kb on - strand, within HMPREF1058_RS06160 at 544.586 kb on + strand, within HMPREF1058_RS06160 at 544.602 kb on - strand, within HMPREF1058_RS06160 at 544.639 kb on + strand, within HMPREF1058_RS06160 at 544.689 kb on - strand, within HMPREF1058_RS06160 at 544.726 kb on - strand at 544.795 kb on - strand at 545.091 kb on - strand, within HMPREF1058_RS06155 at 545.280 kb on - strand, within HMPREF1058_RS06145 at 545.393 kb on + strand at 545.445 kb on + strand at 545.446 kb on - strand at 545.484 kb on - strand at 545.485 kb on + strand at 545.554 kb on - strand at 545.574 kb on - strand at 545.588 kb on - strand at 545.623 kb on - strand at 545.623 kb on - strand at 545.792 kb on + strand, within HMPREF1058_RS06140 at 545.792 kb on + strand, within HMPREF1058_RS06140 at 545.794 kb on + strand, within HMPREF1058_RS06140 at 545.795 kb on - strand, within HMPREF1058_RS06140
Per-strain Table
Position Strand Gene LocusTag Fraction Red Arabinan from sugar-beet; filtered 2.5 mg/ml (C) remove 543,010 - HMPREF1058_RS06170 0.58 -3.5 543,228 + HMPREF1058_RS06170 0.88 -1.8 543,229 - HMPREF1058_RS06170 0.88 -3.5 543,244 + -3.1 543,300 + -2.8 543,301 - -3.3 543,301 - -4.2 543,315 + -2.7 543,316 - -3.5 543,328 - -0.1 543,328 - +1.4 543,335 + -3.2 543,340 - -1.0 543,340 - -0.5 543,342 - +0.5 543,467 - HMPREF1058_RS06165 0.19 -2.9 543,467 - HMPREF1058_RS06165 0.19 -3.9 543,507 + HMPREF1058_RS06165 0.26 -0.6 543,508 - HMPREF1058_RS06165 0.26 -2.5 543,519 - HMPREF1058_RS06165 0.28 -2.6 543,519 - HMPREF1058_RS06165 0.28 -2.4 543,638 + HMPREF1058_RS06165 0.48 -4.6 543,638 + HMPREF1058_RS06165 0.48 -2.2 543,722 + HMPREF1058_RS06165 0.62 -1.6 543,726 - HMPREF1058_RS06165 0.63 -3.7 543,737 + HMPREF1058_RS06165 0.65 -3.1 543,737 + HMPREF1058_RS06165 0.65 -2.4 543,746 - HMPREF1058_RS06165 0.66 -2.0 543,839 + HMPREF1058_RS06165 0.82 -1.4 543,840 - HMPREF1058_RS06165 0.82 -2.2 543,903 + -3.6 543,954 + -5.3 543,955 - -2.5 543,972 - -0.6 543,993 + -2.5 543,993 + -2.9 544,048 + HMPREF1058_RS06160 0.12 -0.9 544,087 + HMPREF1058_RS06160 0.17 -0.1 544,088 - HMPREF1058_RS06160 0.17 -0.9 544,225 + HMPREF1058_RS06160 0.33 -1.9 544,282 - HMPREF1058_RS06160 0.40 +1.1 544,431 + HMPREF1058_RS06160 0.57 -2.5 544,435 + HMPREF1058_RS06160 0.57 -2.2 544,435 + HMPREF1058_RS06160 0.57 -3.9 544,488 + HMPREF1058_RS06160 0.64 -1.6 544,541 - HMPREF1058_RS06160 0.70 -2.2 544,586 + HMPREF1058_RS06160 0.75 -3.7 544,602 - HMPREF1058_RS06160 0.77 -1.7 544,639 + HMPREF1058_RS06160 0.81 -2.9 544,689 - HMPREF1058_RS06160 0.87 -1.5 544,726 - -2.7 544,795 - -3.1 545,091 - HMPREF1058_RS06155 0.35 +0.1 545,280 - HMPREF1058_RS06145 0.11 -1.8 545,393 + -0.9 545,445 + -0.4 545,446 - -0.2 545,484 - -0.9 545,485 + -0.6 545,554 - -0.8 545,574 - -0.4 545,588 - -0.6 545,623 - -0.8 545,623 - -1.1 545,792 + HMPREF1058_RS06140 0.30 -1.6 545,792 + HMPREF1058_RS06140 0.30 -1.8 545,794 + HMPREF1058_RS06140 0.30 -0.6 545,795 - HMPREF1058_RS06140 0.30 -1.4
Or see this region's nucleotide sequence