Experiment: Varel_Bryant_medium_Glucose with Irgasan 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt p5482_20 and p5482_21 are separated by 556 nucleotides p5482_21 and p5482_22 are separated by 82 nucleotides p5482_22 and p5482_23 are separated by 339 nucleotides p5482_23 and p5482_24 are separated by 124 nucleotides
p5482_20: p5482_20 - hypothetical protein (NCBI), at 18,135 to 18,590
_20
p5482_21: p5482_21 - hypothetical protein (NCBI), at 19,147 to 19,419
_21
p5482_22: p5482_22 - hypothetical protein (NCBI), at 19,502 to 19,945
_22
p5482_23: p5482_23 - hypothetical protein (NCBI), at 20,285 to 20,521
_23
p5482_24: p5482_24 - hypothetical protein (NCBI), at 20,646 to 20,846
_24
Position (kb) 19
20 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 18.556 kb on + strand at 18.556 kb on + strand at 18.556 kb on + strand at 18.556 kb on + strand at 18.557 kb on - strand at 18.557 kb on - strand at 18.557 kb on - strand at 18.560 kb on + strand at 18.560 kb on + strand at 18.560 kb on + strand at 18.572 kb on + strand at 18.579 kb on + strand at 18.582 kb on + strand at 18.583 kb on - strand at 18.583 kb on - strand at 18.583 kb on - strand at 18.584 kb on + strand at 18.585 kb on - strand at 18.585 kb on - strand at 18.672 kb on - strand at 18.672 kb on - strand at 18.706 kb on + strand at 18.707 kb on - strand at 18.707 kb on - strand at 18.841 kb on - strand at 18.847 kb on + strand at 18.847 kb on + strand at 18.875 kb on + strand at 18.875 kb on + strand at 18.875 kb on + strand at 18.876 kb on - strand at 18.876 kb on - strand at 18.876 kb on - strand at 18.879 kb on + strand at 18.879 kb on + strand at 18.879 kb on + strand at 18.880 kb on - strand at 18.928 kb on + strand at 18.929 kb on - strand at 18.941 kb on + strand at 18.942 kb on - strand at 18.968 kb on + strand at 18.969 kb on - strand at 18.977 kb on - strand at 18.977 kb on - strand at 18.977 kb on - strand at 18.990 kb on + strand at 18.990 kb on + strand at 18.991 kb on - strand at 18.995 kb on - strand at 18.995 kb on - strand at 18.995 kb on - strand at 18.995 kb on - strand at 19.043 kb on + strand at 19.044 kb on - strand at 19.086 kb on + strand at 19.087 kb on - strand at 19.087 kb on - strand at 19.087 kb on - strand at 19.087 kb on - strand at 19.091 kb on + strand at 19.095 kb on + strand at 19.104 kb on - strand at 19.124 kb on + strand at 19.124 kb on + strand at 19.127 kb on + strand at 19.128 kb on - strand at 19.136 kb on + strand at 19.136 kb on + strand at 19.146 kb on + strand at 19.158 kb on + strand at 19.158 kb on + strand at 19.159 kb on - strand at 19.159 kb on - strand at 19.222 kb on + strand, within p5482_21 at 19.222 kb on + strand, within p5482_21 at 19.224 kb on + strand, within p5482_21 at 19.224 kb on + strand, within p5482_21 at 19.225 kb on - strand, within p5482_21 at 19.238 kb on + strand, within p5482_21 at 19.342 kb on + strand, within p5482_21 at 19.342 kb on + strand, within p5482_21 at 19.343 kb on - strand, within p5482_21 at 19.356 kb on + strand, within p5482_21 at 19.357 kb on - strand, within p5482_21 at 19.450 kb on + strand at 19.530 kb on + strand at 19.530 kb on + strand at 19.530 kb on + strand at 19.530 kb on + strand at 19.530 kb on + strand at 19.531 kb on - strand at 19.558 kb on + strand, within p5482_22 at 19.558 kb on + strand, within p5482_22 at 19.626 kb on + strand at 19.626 kb on + strand, within p5482_22 at 19.627 kb on - strand, within p5482_22 at 19.627 kb on - strand, within p5482_22 at 19.627 kb on - strand, within p5482_22 at 19.627 kb on - strand, within p5482_22 at 19.627 kb on - strand, within p5482_22 at 19.654 kb on + strand, within p5482_22 at 19.685 kb on + strand, within p5482_22 at 19.685 kb on + strand, within p5482_22 at 19.685 kb on + strand, within p5482_22 at 19.686 kb on - strand, within p5482_22 at 19.686 kb on - strand, within p5482_22 at 19.729 kb on + strand, within p5482_22 at 19.730 kb on - strand, within p5482_22 at 19.730 kb on - strand, within p5482_22 at 19.793 kb on + strand, within p5482_22 at 19.853 kb on + strand, within p5482_22 at 19.854 kb on - strand, within p5482_22 at 19.854 kb on - strand, within p5482_22 at 19.854 kb on - strand, within p5482_22 at 19.858 kb on + strand, within p5482_22 at 19.859 kb on - strand, within p5482_22 at 19.859 kb on - strand, within p5482_22 at 19.866 kb on + strand, within p5482_22 at 19.866 kb on + strand, within p5482_22 at 19.866 kb on + strand, within p5482_22 at 19.866 kb on + strand, within p5482_22 at 19.867 kb on - strand, within p5482_22 at 19.867 kb on - strand, within p5482_22 at 19.867 kb on - strand, within p5482_22 at 19.867 kb on - strand, within p5482_22 at 19.894 kb on - strand, within p5482_22 at 19.911 kb on - strand at 19.933 kb on + strand at 19.945 kb on + strand at 19.946 kb on - strand at 19.951 kb on + strand at 19.952 kb on - strand at 19.972 kb on + strand at 20.003 kb on + strand at 20.006 kb on + strand at 20.006 kb on + strand at 20.006 kb on + strand at 20.006 kb on + strand at 20.006 kb on + strand at 20.007 kb on - strand at 20.007 kb on - strand at 20.007 kb on - strand at 20.010 kb on + strand at 20.010 kb on + strand at 20.013 kb on - strand at 20.019 kb on + strand at 20.019 kb on + strand at 20.020 kb on - strand at 20.020 kb on - strand at 20.020 kb on - strand at 20.020 kb on - strand at 20.026 kb on + strand at 20.027 kb on - strand at 20.027 kb on - strand at 20.035 kb on - strand at 20.040 kb on + strand at 20.040 kb on + strand at 20.040 kb on + strand at 20.040 kb on + strand at 20.043 kb on + strand at 20.043 kb on + strand at 20.043 kb on + strand at 20.044 kb on - strand at 20.044 kb on - strand at 20.050 kb on + strand at 20.103 kb on - strand at 20.141 kb on + strand at 20.141 kb on + strand at 20.141 kb on + strand at 20.141 kb on + strand at 20.141 kb on + strand at 20.141 kb on + strand at 20.142 kb on - strand at 20.142 kb on - strand at 20.142 kb on - strand at 20.143 kb on + strand at 20.144 kb on + strand at 20.144 kb on + strand at 20.144 kb on + strand at 20.159 kb on - strand at 20.159 kb on - strand at 20.220 kb on + strand at 20.220 kb on + strand at 20.221 kb on - strand at 20.222 kb on + strand at 20.222 kb on + strand at 20.222 kb on + strand at 20.222 kb on + strand at 20.222 kb on + strand at 20.223 kb on - strand at 20.223 kb on - strand at 20.223 kb on - strand at 20.223 kb on - strand at 20.223 kb on - strand at 20.223 kb on - strand at 20.223 kb on - strand at 20.223 kb on - strand at 20.223 kb on - strand at 20.223 kb on - strand at 20.223 kb on - strand at 20.224 kb on + strand at 20.224 kb on + strand at 20.224 kb on + strand at 20.224 kb on + strand at 20.224 kb on + strand at 20.224 kb on + strand at 20.224 kb on + strand at 20.225 kb on - strand at 20.225 kb on - strand at 20.225 kb on - strand at 20.270 kb on - strand at 20.292 kb on + strand at 20.292 kb on + strand at 20.293 kb on - strand at 20.293 kb on - strand at 20.305 kb on + strand at 20.306 kb on - strand at 20.306 kb on - strand at 20.306 kb on - strand at 20.310 kb on + strand, within p5482_23 at 20.313 kb on - strand, within p5482_23 at 20.313 kb on - strand, within p5482_23 at 20.326 kb on + strand, within p5482_23 at 20.327 kb on - strand, within p5482_23 at 20.327 kb on - strand, within p5482_23 at 20.327 kb on - strand, within p5482_23 at 20.332 kb on + strand, within p5482_23 at 20.388 kb on - strand, within p5482_23 at 20.388 kb on - strand, within p5482_23 at 20.441 kb on + strand, within p5482_23 at 20.459 kb on + strand, within p5482_23 at 20.460 kb on - strand, within p5482_23 at 20.460 kb on - strand, within p5482_23 at 20.471 kb on + strand, within p5482_23 at 20.471 kb on + strand, within p5482_23 at 20.472 kb on - strand, within p5482_23 at 20.474 kb on + strand, within p5482_23 at 20.475 kb on - strand, within p5482_23 at 20.516 kb on - strand at 20.521 kb on + strand at 20.521 kb on + strand at 20.521 kb on + strand at 20.522 kb on - strand at 20.522 kb on - strand at 20.522 kb on - strand at 20.529 kb on + strand at 20.530 kb on - strand at 20.537 kb on - strand at 20.538 kb on + strand at 20.580 kb on + strand at 20.580 kb on + strand at 20.580 kb on + strand at 20.588 kb on + strand at 20.588 kb on + strand at 20.588 kb on + strand at 20.589 kb on - strand at 20.589 kb on - strand at 20.589 kb on - strand at 20.594 kb on + strand at 20.627 kb on - strand at 20.637 kb on + strand at 20.644 kb on + strand at 20.644 kb on + strand at 20.644 kb on + strand at 20.644 kb on + strand at 20.645 kb on - strand at 20.645 kb on - strand at 20.645 kb on - strand at 20.645 kb on - strand at 20.660 kb on + strand at 20.661 kb on - strand at 20.661 kb on - strand at 20.661 kb on - strand at 20.662 kb on + strand at 20.662 kb on + strand at 20.663 kb on - strand at 20.663 kb on - strand at 20.663 kb on - strand at 20.663 kb on - strand at 20.689 kb on + strand, within p5482_24 at 20.689 kb on + strand, within p5482_24 at 20.689 kb on + strand, within p5482_24 at 20.689 kb on + strand, within p5482_24 at 20.689 kb on + strand, within p5482_24 at 20.689 kb on + strand, within p5482_24 at 20.690 kb on - strand, within p5482_24 at 20.690 kb on - strand, within p5482_24 at 20.690 kb on - strand, within p5482_24 at 20.691 kb on + strand, within p5482_24 at 20.691 kb on + strand, within p5482_24 at 20.691 kb on + strand, within p5482_24 at 20.691 kb on + strand, within p5482_24 at 20.692 kb on - strand, within p5482_24 at 20.696 kb on + strand, within p5482_24 at 20.696 kb on + strand, within p5482_24 at 20.751 kb on + strand at 20.778 kb on + strand, within p5482_24 at 20.778 kb on + strand, within p5482_24 at 20.780 kb on + strand, within p5482_24 at 20.780 kb on + strand, within p5482_24 at 20.780 kb on + strand, within p5482_24 at 20.780 kb on + strand, within p5482_24 at 20.780 kb on + strand, within p5482_24 at 20.780 kb on + strand, within p5482_24 at 20.781 kb on - strand, within p5482_24 at 20.781 kb on - strand, within p5482_24 at 20.781 kb on - strand, within p5482_24 at 20.781 kb on - strand, within p5482_24 at 20.781 kb on - strand, within p5482_24 at 20.781 kb on - strand, within p5482_24 at 20.822 kb on + strand, within p5482_24 at 20.822 kb on + strand, within p5482_24 at 20.822 kb on + strand, within p5482_24 at 20.823 kb on - strand, within p5482_24 at 20.829 kb on + strand at 20.829 kb on + strand at 20.829 kb on + strand at 20.830 kb on - strand at 20.830 kb on - strand at 20.830 kb on - strand at 20.830 kb on - strand at 20.831 kb on + strand at 20.831 kb on + strand at 20.831 kb on + strand at 20.832 kb on - strand at 20.850 kb on - strand at 20.863 kb on + strand at 20.877 kb on + strand at 20.882 kb on - strand at 20.882 kb on - strand at 20.885 kb on + strand at 20.885 kb on + strand at 20.885 kb on + strand at 20.886 kb on - strand at 20.886 kb on - strand at 20.912 kb on + strand at 20.912 kb on + strand at 20.913 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Irgasan 0.025 mM remove 18,556 + -0.9 18,556 + -2.4 18,556 + -2.4 18,556 + -1.4 18,557 - -1.0 18,557 - -1.3 18,557 - -1.0 18,560 + -1.7 18,560 + +1.4 18,560 + -0.8 18,572 + -0.9 18,579 + -0.4 18,582 + -1.7 18,583 - -0.6 18,583 - -1.5 18,583 - -1.4 18,584 + -0.5 18,585 - -0.4 18,585 - -0.4 18,672 - -3.6 18,672 - -0.2 18,706 + -0.6 18,707 - -0.9 18,707 - -2.0 18,841 - +2.2 18,847 + -1.7 18,847 + -1.4 18,875 + -0.5 18,875 + -1.4 18,875 + -0.7 18,876 - -0.3 18,876 - +0.0 18,876 - +0.2 18,879 + -0.7 18,879 + -2.7 18,879 + -1.2 18,880 - -2.1 18,928 + -2.2 18,929 - -3.5 18,941 + -0.9 18,942 - -1.4 18,968 + +0.2 18,969 - -0.4 18,977 - -1.9 18,977 - -2.2 18,977 - -1.4 18,990 + -0.8 18,990 + -1.8 18,991 - -1.0 18,995 - -0.9 18,995 - -1.0 18,995 - -1.3 18,995 - -1.0 19,043 + +1.6 19,044 - +2.2 19,086 + -1.0 19,087 - -1.2 19,087 - -0.7 19,087 - -1.5 19,087 - -0.4 19,091 + -1.5 19,095 + -0.8 19,104 - -0.0 19,124 + -2.1 19,124 + -0.4 19,127 + -1.4 19,128 - -0.6 19,136 + -0.8 19,136 + -1.0 19,146 + -2.5 19,158 + -2.7 19,158 + -2.2 19,159 - +0.4 19,159 - -0.8 19,222 + p5482_21 0.27 -0.7 19,222 + p5482_21 0.27 -1.4 19,224 + p5482_21 0.28 -2.5 19,224 + p5482_21 0.28 -0.3 19,225 - p5482_21 0.29 +0.6 19,238 + p5482_21 0.33 -0.7 19,342 + p5482_21 0.71 -0.4 19,342 + p5482_21 0.71 +0.9 19,343 - p5482_21 0.72 -0.1 19,356 + p5482_21 0.77 -0.8 19,357 - p5482_21 0.77 -1.4 19,450 + -1.0 19,530 + -0.2 19,530 + -0.4 19,530 + -2.0 19,530 + +0.8 19,530 + -1.6 19,531 - +1.6 19,558 + p5482_22 0.13 -0.6 19,558 + p5482_22 0.13 -1.8 19,626 + +0.6 19,626 + p5482_22 0.28 +0.9 19,627 - p5482_22 0.28 -2.4 19,627 - p5482_22 0.28 +2.4 19,627 - p5482_22 0.28 -0.4 19,627 - p5482_22 0.28 -0.0 19,627 - p5482_22 0.28 +1.6 19,654 + p5482_22 0.34 -0.6 19,685 + p5482_22 0.41 +0.6 19,685 + p5482_22 0.41 -1.4 19,685 + p5482_22 0.41 -0.2 19,686 - p5482_22 0.41 -0.7 19,686 - p5482_22 0.41 -2.0 19,729 + p5482_22 0.51 +1.1 19,730 - p5482_22 0.51 -0.7 19,730 - p5482_22 0.51 -0.8 19,793 + p5482_22 0.66 -3.2 19,853 + p5482_22 0.79 -0.2 19,854 - p5482_22 0.79 -0.1 19,854 - p5482_22 0.79 -0.6 19,854 - p5482_22 0.79 -2.2 19,858 + p5482_22 0.80 -0.8 19,859 - p5482_22 0.80 -0.7 19,859 - p5482_22 0.80 -1.0 19,866 + p5482_22 0.82 -1.4 19,866 + p5482_22 0.82 -0.4 19,866 + p5482_22 0.82 -2.2 19,866 + p5482_22 0.82 -0.9 19,867 - p5482_22 0.82 -2.7 19,867 - p5482_22 0.82 +0.8 19,867 - p5482_22 0.82 -0.4 19,867 - p5482_22 0.82 +0.6 19,894 - p5482_22 0.88 +2.4 19,911 - +1.4 19,933 + -1.7 19,945 + -0.8 19,946 - -1.2 19,951 + -1.1 19,952 - -1.1 19,972 + -2.0 20,003 + -2.3 20,006 + -0.9 20,006 + -1.0 20,006 + -1.1 20,006 + -0.9 20,006 + -1.0 20,007 - -0.4 20,007 - +0.6 20,007 - -0.8 20,010 + -1.0 20,010 + -1.0 20,013 - -1.1 20,019 + -1.2 20,019 + -1.2 20,020 - -0.7 20,020 - -0.4 20,020 - -0.8 20,020 - -3.3 20,026 + -1.4 20,027 - -1.1 20,027 - -2.5 20,035 - -1.1 20,040 + +0.3 20,040 + -0.8 20,040 + -1.2 20,040 + -0.6 20,043 + -1.2 20,043 + -1.4 20,043 + -0.1 20,044 - -0.4 20,044 - -0.7 20,050 + -1.2 20,103 - -1.0 20,141 + -0.5 20,141 + -1.1 20,141 + +0.2 20,141 + -1.0 20,141 + +0.2 20,141 + -1.3 20,142 - -1.7 20,142 - -1.0 20,142 - -1.1 20,143 + -1.2 20,144 + -0.4 20,144 + -0.7 20,144 + -0.8 20,159 - -0.4 20,159 - -0.9 20,220 + -1.9 20,220 + -1.0 20,221 - -0.4 20,222 + -1.6 20,222 + +0.6 20,222 + -2.2 20,222 + -2.0 20,222 + -0.5 20,223 - -0.5 20,223 - -1.0 20,223 - -0.9 20,223 - -0.6 20,223 - -1.0 20,223 - -0.4 20,223 - -1.5 20,223 - -0.1 20,223 - -0.3 20,223 - -1.8 20,223 - +0.6 20,224 + -1.0 20,224 + -2.0 20,224 + -0.3 20,224 + -1.1 20,224 + -1.0 20,224 + -1.6 20,224 + -0.3 20,225 - -1.0 20,225 - -0.7 20,225 - -2.5 20,270 - +0.6 20,292 + -0.7 20,292 + +0.3 20,293 - -0.4 20,293 - +0.6 20,305 + -0.9 20,306 - -0.1 20,306 - -0.3 20,306 - -1.9 20,310 + p5482_23 0.11 -1.1 20,313 - p5482_23 0.12 -3.0 20,313 - p5482_23 0.12 -0.4 20,326 + p5482_23 0.17 -0.2 20,327 - p5482_23 0.18 -1.3 20,327 - p5482_23 0.18 -1.1 20,327 - p5482_23 0.18 +1.6 20,332 + p5482_23 0.20 -1.0 20,388 - p5482_23 0.43 -0.6 20,388 - p5482_23 0.43 -1.5 20,441 + p5482_23 0.66 -1.7 20,459 + p5482_23 0.73 -2.1 20,460 - p5482_23 0.74 -1.3 20,460 - p5482_23 0.74 -1.4 20,471 + p5482_23 0.78 +0.1 20,471 + p5482_23 0.78 -0.6 20,472 - p5482_23 0.79 -1.5 20,474 + p5482_23 0.80 +0.6 20,475 - p5482_23 0.80 -1.3 20,516 - -1.7 20,521 + -0.9 20,521 + -2.0 20,521 + -0.4 20,522 - -0.4 20,522 - -2.5 20,522 - -1.4 20,529 + -0.5 20,530 - -0.4 20,537 - -0.0 20,538 + -1.0 20,580 + -1.0 20,580 + -2.0 20,580 + -0.8 20,588 + -2.2 20,588 + -0.5 20,588 + -1.4 20,589 - +0.0 20,589 - +0.2 20,589 - -0.3 20,594 + -3.0 20,627 - +0.9 20,637 + -1.0 20,644 + -1.0 20,644 + -2.3 20,644 + +0.0 20,644 + -0.9 20,645 - -1.9 20,645 - -0.6 20,645 - -0.4 20,645 - -1.4 20,660 + -1.0 20,661 - -1.1 20,661 - -0.0 20,661 - +0.6 20,662 + -2.1 20,662 + -1.1 20,663 - -0.1 20,663 - -1.2 20,663 - -0.3 20,663 - -0.4 20,689 + p5482_24 0.21 -0.9 20,689 + p5482_24 0.21 +0.3 20,689 + p5482_24 0.21 -2.8 20,689 + p5482_24 0.21 -0.7 20,689 + p5482_24 0.21 -1.3 20,689 + p5482_24 0.21 +1.5 20,690 - p5482_24 0.22 -1.8 20,690 - p5482_24 0.22 -1.4 20,690 - p5482_24 0.22 -1.4 20,691 + p5482_24 0.22 -0.4 20,691 + p5482_24 0.22 -0.7 20,691 + p5482_24 0.22 +0.2 20,691 + p5482_24 0.22 -2.0 20,692 - p5482_24 0.23 -0.4 20,696 + p5482_24 0.25 -0.8 20,696 + p5482_24 0.25 -1.2 20,751 + -0.4 20,778 + p5482_24 0.66 -1.2 20,778 + p5482_24 0.66 -1.2 20,780 + p5482_24 0.67 -1.1 20,780 + p5482_24 0.67 -0.4 20,780 + p5482_24 0.67 -1.0 20,780 + p5482_24 0.67 -1.5 20,780 + p5482_24 0.67 -0.5 20,780 + p5482_24 0.67 -2.0 20,781 - p5482_24 0.67 +1.2 20,781 - p5482_24 0.67 -0.6 20,781 - p5482_24 0.67 -1.7 20,781 - p5482_24 0.67 -1.4 20,781 - p5482_24 0.67 -1.1 20,781 - p5482_24 0.67 -1.5 20,822 + p5482_24 0.88 -0.5 20,822 + p5482_24 0.88 -0.4 20,822 + p5482_24 0.88 -0.4 20,823 - p5482_24 0.88 -3.0 20,829 + +0.0 20,829 + -1.7 20,829 + -0.7 20,830 - -3.4 20,830 - -0.6 20,830 - -0.7 20,830 - -0.5 20,831 + +0.6 20,831 + -0.4 20,831 + +0.1 20,832 - -4.4 20,850 - -0.2 20,863 + -1.0 20,877 + -1.2 20,882 - -2.7 20,882 - -2.2 20,885 + +1.6 20,885 + -2.3 20,885 + +0.6 20,886 - -1.9 20,886 - -3.0 20,912 + -0.8 20,912 + +0.6 20,913 - -2.0
Or see this region's nucleotide sequence