Experiment: Varel_Bryant_medium_Glucose with Dimethyl Sulfoxide 0.1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3973 and tRNA-Pro are separated by 55 nucleotides tRNA-Pro and BT3974 are separated by 364 nucleotides BT3974 and BT3975 are separated by 123 nucleotides
BT3973: BT3973 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI ptt file), at 5,173,216 to 5,174,436
BT3973
BT_t64: tRNA-Pro - tRNA-Pro (RefSeq), at 5,174,492 to 5,174,568
tRNA-Pro
BT3974: BT3974 - conserved hypothetical protein (NCBI ptt file), at 5,174,933 to 5,175,727
BT3974
BT3975: BT3975 - 3-dehydroquinate synthase (NCBI ptt file), at 5,175,851 to 5,176,912
BT3975
Position (kb)
5174
5175
5176 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5173.960 kb on + strand, within BT3973 at 5174.050 kb on + strand, within BT3973 at 5174.357 kb on - strand at 5174.725 kb on - strand at 5174.747 kb on + strand at 5174.811 kb on + strand at 5174.870 kb on - strand at 5174.873 kb on - strand at 5174.897 kb on + strand at 5174.966 kb on - strand at 5174.968 kb on + strand at 5175.017 kb on - strand, within BT3974 at 5175.021 kb on + strand, within BT3974 at 5175.121 kb on + strand, within BT3974 at 5175.122 kb on - strand, within BT3974 at 5175.200 kb on - strand, within BT3974 at 5175.277 kb on + strand, within BT3974 at 5175.296 kb on - strand, within BT3974 at 5175.297 kb on + strand, within BT3974 at 5175.297 kb on + strand, within BT3974 at 5175.297 kb on + strand, within BT3974 at 5175.297 kb on + strand, within BT3974 at 5175.298 kb on - strand, within BT3974 at 5175.299 kb on + strand, within BT3974 at 5175.300 kb on - strand, within BT3974 at 5175.333 kb on - strand, within BT3974 at 5175.336 kb on + strand, within BT3974 at 5175.404 kb on - strand, within BT3974 at 5175.438 kb on + strand, within BT3974 at 5175.461 kb on - strand, within BT3974 at 5175.485 kb on - strand, within BT3974 at 5175.485 kb on - strand, within BT3974 at 5175.562 kb on - strand, within BT3974 at 5175.573 kb on + strand, within BT3974 at 5175.573 kb on + strand, within BT3974 at 5175.613 kb on + strand, within BT3974 at 5175.614 kb on - strand, within BT3974 at 5175.715 kb on + strand at 5175.716 kb on - strand at 5175.731 kb on - strand at 5175.831 kb on + strand at 5175.831 kb on + strand at 5175.863 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Dimethyl Sulfoxide 0.1 vol% remove 5,173,960 + BT3973 0.61 -3.2 5,174,050 + BT3973 0.68 -0.7 5,174,357 - -3.2 5,174,725 - -1.6 5,174,747 + -1.5 5,174,811 + -0.8 5,174,870 - -1.0 5,174,873 - +0.1 5,174,897 + +0.1 5,174,966 - +0.1 5,174,968 + -1.0 5,175,017 - BT3974 0.11 +1.6 5,175,021 + BT3974 0.11 +1.1 5,175,121 + BT3974 0.24 -0.0 5,175,122 - BT3974 0.24 +1.0 5,175,200 - BT3974 0.34 +0.3 5,175,277 + BT3974 0.43 -0.1 5,175,296 - BT3974 0.46 -0.0 5,175,297 + BT3974 0.46 -0.6 5,175,297 + BT3974 0.46 +0.6 5,175,297 + BT3974 0.46 -0.6 5,175,297 + BT3974 0.46 -0.0 5,175,298 - BT3974 0.46 -2.9 5,175,299 + BT3974 0.46 +1.5 5,175,300 - BT3974 0.46 -2.6 5,175,333 - BT3974 0.50 +2.0 5,175,336 + BT3974 0.51 -1.4 5,175,404 - BT3974 0.59 +0.3 5,175,438 + BT3974 0.64 +0.4 5,175,461 - BT3974 0.66 +0.5 5,175,485 - BT3974 0.69 -0.2 5,175,485 - BT3974 0.69 +0.5 5,175,562 - BT3974 0.79 -2.4 5,175,573 + BT3974 0.81 +0.3 5,175,573 + BT3974 0.81 +2.4 5,175,613 + BT3974 0.86 +0.6 5,175,614 - BT3974 0.86 +2.1 5,175,715 + +1.5 5,175,716 - -2.6 5,175,731 - -0.5 5,175,831 + -0.6 5,175,831 + +0.8 5,175,863 - -0.9
Or see this region's nucleotide sequence