Experiment: No vitamins, second passage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_460 and HP15_461 are separated by 8 nucleotides HP15_461 and HP15_462 are separated by 482 nucleotides HP15_462 and HP15_463 are separated by 15 nucleotides
HP15_460: HP15_460 - SAM-dependent methyltransferase, at 465,530 to 466,735
_460
HP15_461: HP15_461 - Histidinol-phosphatase (EC:3.1.3.15) <small>(from data)</small>, at 466,744 to 467,400
_461
HP15_462: HP15_462 - dinucleoside polyphosphate hydrolase, at 467,883 to 468,104
_462
HP15_463: HP15_463 - fused phosphoenolpyruvate-protein phosphotransferase PtsP/GAF domain protein, at 468,120 to 470,426
_463
Position (kb) 466
467
468 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 465.758 kb on - strand, within HP15_460 at 465.758 kb on - strand, within HP15_460 at 465.944 kb on - strand, within HP15_460 at 465.966 kb on + strand, within HP15_460 at 465.971 kb on + strand, within HP15_460 at 466.424 kb on - strand, within HP15_460 at 466.488 kb on - strand, within HP15_460 at 466.489 kb on - strand, within HP15_460 at 466.500 kb on + strand, within HP15_460 at 466.513 kb on - strand, within HP15_460 at 467.084 kb on + strand, within HP15_461 at 467.448 kb on + strand at 467.456 kb on - strand at 467.572 kb on - strand at 467.590 kb on + strand at 467.598 kb on - strand at 467.992 kb on + strand, within HP15_462 at 468.038 kb on + strand, within HP15_462 at 468.069 kb on + strand, within HP15_462 at 468.139 kb on + strand at 468.245 kb on + strand at 468.328 kb on + strand at 468.331 kb on - strand at 468.336 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins, second passage remove 465,758 - HP15_460 0.19 +1.5 465,758 - HP15_460 0.19 +0.1 465,944 - HP15_460 0.34 +0.2 465,966 + HP15_460 0.36 +1.9 465,971 + HP15_460 0.37 -0.1 466,424 - HP15_460 0.74 +1.4 466,488 - HP15_460 0.79 -0.7 466,489 - HP15_460 0.80 -0.2 466,500 + HP15_460 0.80 +0.3 466,513 - HP15_460 0.82 -0.7 467,084 + HP15_461 0.52 -2.9 467,448 + -0.3 467,456 - +0.3 467,572 - -0.2 467,590 + +0.5 467,598 - -1.1 467,992 + HP15_462 0.49 -1.5 468,038 + HP15_462 0.70 +0.4 468,069 + HP15_462 0.84 -1.1 468,139 + +1.8 468,245 + -1.9 468,328 + +0.6 468,331 - -1.4 468,336 - +0.5
Or see this region's nucleotide sequence