Experiment: No vitamins, second passage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_4014 and HP15_4015 overlap by 7 nucleotides HP15_4015 and HP15_4016 are separated by 16 nucleotides HP15_4016 and HP15_4017 are separated by 168 nucleotides
HP15_4014: HP15_4014 - FAD dependent oxidoreductase, at 4,247,803 to 4,249,461
_4014
HP15_4015: HP15_4015 - conserved hypothetical protein, at 4,249,455 to 4,249,961
_4015
HP15_4016: HP15_4016 - deoxyribodipyrimidine photolyase, at 4,249,978 to 4,251,300
_4016
HP15_4017: HP15_4017 - formaldehyde dehydrogenase (glutathione-dependent), at 4,251,469 to 4,252,581
_4017
Position (kb)
4249
4250
4251
4252 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4248.989 kb on - strand, within HP15_4014 at 4248.989 kb on - strand, within HP15_4014 at 4249.022 kb on + strand, within HP15_4014 at 4249.029 kb on + strand, within HP15_4014 at 4249.186 kb on - strand, within HP15_4014 at 4249.259 kb on + strand, within HP15_4014 at 4249.259 kb on + strand, within HP15_4014 at 4249.267 kb on - strand, within HP15_4014 at 4249.319 kb on - strand at 4249.326 kb on - strand at 4249.438 kb on + strand at 4249.489 kb on + strand at 4249.489 kb on + strand at 4249.497 kb on - strand at 4249.514 kb on - strand, within HP15_4015 at 4249.515 kb on - strand, within HP15_4015 at 4249.539 kb on - strand, within HP15_4015 at 4249.554 kb on - strand, within HP15_4015 at 4249.556 kb on - strand, within HP15_4015 at 4249.623 kb on + strand, within HP15_4015 at 4249.691 kb on + strand, within HP15_4015 at 4249.729 kb on - strand, within HP15_4015 at 4249.756 kb on - strand, within HP15_4015 at 4249.772 kb on + strand, within HP15_4015 at 4249.780 kb on - strand, within HP15_4015 at 4249.908 kb on + strand, within HP15_4015 at 4250.008 kb on + strand at 4250.008 kb on + strand at 4250.016 kb on - strand at 4250.016 kb on - strand at 4250.016 kb on - strand at 4250.016 kb on - strand at 4250.016 kb on - strand at 4250.016 kb on - strand at 4250.016 kb on - strand at 4250.070 kb on - strand at 4250.111 kb on + strand, within HP15_4016 at 4250.119 kb on - strand, within HP15_4016 at 4250.338 kb on - strand, within HP15_4016 at 4250.427 kb on + strand, within HP15_4016 at 4250.435 kb on - strand, within HP15_4016 at 4250.466 kb on + strand, within HP15_4016 at 4250.578 kb on + strand, within HP15_4016 at 4250.581 kb on - strand, within HP15_4016 at 4250.636 kb on + strand, within HP15_4016 at 4250.701 kb on - strand, within HP15_4016 at 4250.805 kb on + strand, within HP15_4016 at 4250.825 kb on - strand, within HP15_4016 at 4250.916 kb on - strand, within HP15_4016 at 4251.020 kb on - strand, within HP15_4016 at 4251.020 kb on - strand, within HP15_4016 at 4251.101 kb on - strand, within HP15_4016 at 4251.160 kb on - strand, within HP15_4016 at 4251.166 kb on + strand, within HP15_4016 at 4251.210 kb on + strand at 4251.217 kb on - strand at 4251.276 kb on + strand at 4251.283 kb on + strand at 4251.471 kb on + strand at 4251.559 kb on + strand at 4251.602 kb on + strand, within HP15_4017 at 4251.631 kb on + strand, within HP15_4017 at 4251.794 kb on - strand, within HP15_4017 at 4251.818 kb on - strand, within HP15_4017 at 4251.884 kb on + strand, within HP15_4017 at 4251.949 kb on + strand, within HP15_4017 at 4252.003 kb on + strand, within HP15_4017 at 4252.003 kb on + strand, within HP15_4017 at 4252.005 kb on + strand, within HP15_4017 at 4252.011 kb on - strand, within HP15_4017 at 4252.011 kb on - strand, within HP15_4017 at 4252.011 kb on - strand, within HP15_4017 at 4252.011 kb on - strand, within HP15_4017 at 4252.011 kb on - strand, within HP15_4017 at 4252.011 kb on - strand, within HP15_4017 at 4252.011 kb on - strand, within HP15_4017 at 4252.011 kb on - strand, within HP15_4017 at 4252.021 kb on + strand, within HP15_4017 at 4252.029 kb on - strand, within HP15_4017 at 4252.233 kb on + strand, within HP15_4017 at 4252.266 kb on - strand, within HP15_4017
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins, second passage remove 4,248,989 - HP15_4014 0.71 +1.0 4,248,989 - HP15_4014 0.71 -0.1 4,249,022 + HP15_4014 0.73 -0.5 4,249,029 + HP15_4014 0.74 +1.0 4,249,186 - HP15_4014 0.83 -0.5 4,249,259 + HP15_4014 0.88 -0.3 4,249,259 + HP15_4014 0.88 +0.4 4,249,267 - HP15_4014 0.88 -0.4 4,249,319 - +0.5 4,249,326 - +1.3 4,249,438 + -0.5 4,249,489 + +2.2 4,249,489 + +0.2 4,249,497 - -0.8 4,249,514 - HP15_4015 0.12 -1.1 4,249,515 - HP15_4015 0.12 +1.8 4,249,539 - HP15_4015 0.17 -0.5 4,249,554 - HP15_4015 0.20 -0.6 4,249,556 - HP15_4015 0.20 -1.3 4,249,623 + HP15_4015 0.33 +1.2 4,249,691 + HP15_4015 0.47 +0.8 4,249,729 - HP15_4015 0.54 -0.5 4,249,756 - HP15_4015 0.59 +0.8 4,249,772 + HP15_4015 0.63 -0.5 4,249,780 - HP15_4015 0.64 +1.6 4,249,908 + HP15_4015 0.89 -0.6 4,250,008 + +1.0 4,250,008 + +1.5 4,250,016 - -0.5 4,250,016 - -0.4 4,250,016 - +0.1 4,250,016 - -0.5 4,250,016 - -0.1 4,250,016 - -0.7 4,250,016 - -0.3 4,250,070 - +0.1 4,250,111 + HP15_4016 0.10 -0.7 4,250,119 - HP15_4016 0.11 +0.6 4,250,338 - HP15_4016 0.27 +0.2 4,250,427 + HP15_4016 0.34 +1.6 4,250,435 - HP15_4016 0.35 -0.6 4,250,466 + HP15_4016 0.37 -0.4 4,250,578 + HP15_4016 0.45 -0.4 4,250,581 - HP15_4016 0.46 -0.6 4,250,636 + HP15_4016 0.50 -0.3 4,250,701 - HP15_4016 0.55 -0.5 4,250,805 + HP15_4016 0.63 +0.1 4,250,825 - HP15_4016 0.64 -0.5 4,250,916 - HP15_4016 0.71 +1.0 4,251,020 - HP15_4016 0.79 +1.1 4,251,020 - HP15_4016 0.79 +2.8 4,251,101 - HP15_4016 0.85 -0.0 4,251,160 - HP15_4016 0.89 +0.2 4,251,166 + HP15_4016 0.90 +0.6 4,251,210 + -1.3 4,251,217 - -0.3 4,251,276 + +0.7 4,251,283 + +0.5 4,251,471 + +1.2 4,251,559 + +0.7 4,251,602 + HP15_4017 0.12 -0.3 4,251,631 + HP15_4017 0.15 -0.4 4,251,794 - HP15_4017 0.29 -0.2 4,251,818 - HP15_4017 0.31 +0.1 4,251,884 + HP15_4017 0.37 +0.8 4,251,949 + HP15_4017 0.43 -0.6 4,252,003 + HP15_4017 0.48 -0.4 4,252,003 + HP15_4017 0.48 -0.6 4,252,005 + HP15_4017 0.48 -0.7 4,252,011 - HP15_4017 0.49 -0.1 4,252,011 - HP15_4017 0.49 -0.5 4,252,011 - HP15_4017 0.49 -1.2 4,252,011 - HP15_4017 0.49 -0.1 4,252,011 - HP15_4017 0.49 +0.9 4,252,011 - HP15_4017 0.49 +1.3 4,252,011 - HP15_4017 0.49 -1.6 4,252,011 - HP15_4017 0.49 -2.1 4,252,021 + HP15_4017 0.50 +0.8 4,252,029 - HP15_4017 0.50 -0.4 4,252,233 + HP15_4017 0.69 +0.5 4,252,266 - HP15_4017 0.72 +1.5
Or see this region's nucleotide sequence