Experiment: No vitamins, second passage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_38 and HP15_39 are separated by 235 nucleotides HP15_39 and HP15_40 are separated by 430 nucleotides HP15_40 and HP15_41 are separated by 156 nucleotides
HP15_38: HP15_38 - fumarylacetoacetate (FAA) hydrolase, at 42,075 to 43,064
_38
HP15_39: HP15_39 - ring hydroxylating dioxygenase, alpha subunit, at 43,300 to 44,616
_39
HP15_40: HP15_40 - dioxygenase DitA oxygenase component small subunit-like protein, at 45,047 to 45,211
_40
HP15_41: HP15_41 - conserved hypothetical protein, at 45,368 to 45,718
_41
Position (kb)
43
44
45 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 42.314 kb on - strand, within HP15_38 at 42.375 kb on + strand, within HP15_38 at 42.403 kb on + strand, within HP15_38 at 42.415 kb on + strand, within HP15_38 at 42.426 kb on - strand, within HP15_38 at 42.688 kb on + strand, within HP15_38 at 42.749 kb on - strand, within HP15_38 at 42.769 kb on + strand, within HP15_38 at 42.840 kb on + strand, within HP15_38 at 42.887 kb on + strand, within HP15_38 at 42.915 kb on + strand, within HP15_38 at 42.915 kb on + strand, within HP15_38 at 42.923 kb on - strand, within HP15_38 at 42.949 kb on + strand, within HP15_38 at 42.957 kb on - strand, within HP15_38 at 43.025 kb on - strand at 43.100 kb on + strand at 43.108 kb on - strand at 43.127 kb on - strand at 43.150 kb on + strand at 43.158 kb on - strand at 43.158 kb on - strand at 43.182 kb on - strand at 43.187 kb on + strand at 43.195 kb on - strand at 43.202 kb on - strand at 43.253 kb on + strand at 43.261 kb on + strand at 43.261 kb on - strand at 43.267 kb on + strand at 43.272 kb on + strand at 43.272 kb on + strand at 43.280 kb on - strand at 43.294 kb on + strand at 43.302 kb on - strand at 43.414 kb on - strand at 43.434 kb on - strand, within HP15_39 at 43.434 kb on - strand, within HP15_39 at 43.434 kb on - strand, within HP15_39 at 43.485 kb on - strand, within HP15_39 at 43.595 kb on - strand, within HP15_39 at 43.595 kb on - strand, within HP15_39 at 43.633 kb on - strand, within HP15_39 at 43.677 kb on + strand, within HP15_39 at 43.683 kb on - strand, within HP15_39 at 43.683 kb on - strand, within HP15_39 at 43.692 kb on - strand, within HP15_39 at 43.940 kb on - strand, within HP15_39 at 43.941 kb on - strand, within HP15_39 at 43.946 kb on + strand, within HP15_39 at 43.951 kb on + strand, within HP15_39 at 43.951 kb on + strand, within HP15_39 at 43.951 kb on + strand, within HP15_39 at 43.954 kb on - strand, within HP15_39 at 43.959 kb on - strand, within HP15_39 at 43.959 kb on - strand, within HP15_39 at 43.959 kb on - strand, within HP15_39 at 43.959 kb on - strand, within HP15_39 at 43.973 kb on + strand, within HP15_39 at 43.973 kb on + strand, within HP15_39 at 43.990 kb on + strand, within HP15_39 at 43.990 kb on + strand, within HP15_39 at 43.990 kb on + strand, within HP15_39 at 43.998 kb on - strand, within HP15_39 at 43.998 kb on - strand, within HP15_39 at 43.998 kb on - strand, within HP15_39 at 44.007 kb on + strand, within HP15_39 at 44.012 kb on + strand, within HP15_39 at 44.091 kb on + strand, within HP15_39 at 44.152 kb on - strand, within HP15_39 at 44.164 kb on + strand, within HP15_39 at 44.165 kb on - strand, within HP15_39 at 44.179 kb on - strand, within HP15_39 at 44.214 kb on + strand, within HP15_39 at 44.254 kb on - strand, within HP15_39 at 44.274 kb on + strand, within HP15_39 at 44.276 kb on + strand, within HP15_39 at 44.282 kb on - strand, within HP15_39 at 44.284 kb on - strand, within HP15_39 at 44.284 kb on - strand, within HP15_39 at 44.284 kb on - strand, within HP15_39 at 44.284 kb on - strand, within HP15_39 at 44.377 kb on + strand, within HP15_39 at 44.401 kb on - strand, within HP15_39 at 44.412 kb on + strand, within HP15_39 at 44.412 kb on + strand, within HP15_39 at 44.420 kb on - strand, within HP15_39 at 44.434 kb on + strand, within HP15_39 at 44.604 kb on - strand at 44.611 kb on + strand at 44.633 kb on + strand at 44.661 kb on - strand at 44.709 kb on + strand at 44.709 kb on + strand at 44.709 kb on + strand at 44.716 kb on - strand at 44.717 kb on - strand at 44.807 kb on + strand at 44.825 kb on + strand at 44.887 kb on - strand at 44.904 kb on + strand at 44.909 kb on + strand at 44.912 kb on - strand at 45.091 kb on - strand, within HP15_40 at 45.101 kb on - strand, within HP15_40 at 45.142 kb on - strand, within HP15_40 at 45.143 kb on - strand, within HP15_40 at 45.308 kb on - strand at 45.473 kb on - strand, within HP15_41 at 45.568 kb on + strand, within HP15_41 at 45.577 kb on - strand, within HP15_41 at 45.587 kb on - strand, within HP15_41
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins, second passage remove 42,314 - HP15_38 0.24 +4.1 42,375 + HP15_38 0.30 -0.5 42,403 + HP15_38 0.33 -0.3 42,415 + HP15_38 0.34 -0.1 42,426 - HP15_38 0.35 +1.5 42,688 + HP15_38 0.62 +0.0 42,749 - HP15_38 0.68 +0.5 42,769 + HP15_38 0.70 +1.1 42,840 + HP15_38 0.77 -0.9 42,887 + HP15_38 0.82 +0.4 42,915 + HP15_38 0.85 -0.3 42,915 + HP15_38 0.85 +2.0 42,923 - HP15_38 0.86 -1.3 42,949 + HP15_38 0.88 -0.0 42,957 - HP15_38 0.89 -0.9 43,025 - +0.6 43,100 + +0.7 43,108 - -0.4 43,127 - +0.9 43,150 + +1.0 43,158 - +0.7 43,158 - -0.2 43,182 - -0.0 43,187 + +1.5 43,195 - -0.2 43,202 - -0.5 43,253 + +0.4 43,261 + +0.4 43,261 - +1.4 43,267 + +1.4 43,272 + -0.4 43,272 + +1.9 43,280 - -1.6 43,294 + +0.4 43,302 - +1.4 43,414 - +1.1 43,434 - HP15_39 0.10 +0.0 43,434 - HP15_39 0.10 -0.1 43,434 - HP15_39 0.10 +0.0 43,485 - HP15_39 0.14 -1.2 43,595 - HP15_39 0.22 +0.9 43,595 - HP15_39 0.22 +0.8 43,633 - HP15_39 0.25 -0.0 43,677 + HP15_39 0.29 -0.0 43,683 - HP15_39 0.29 +2.1 43,683 - HP15_39 0.29 -0.9 43,692 - HP15_39 0.30 +1.8 43,940 - HP15_39 0.49 -1.1 43,941 - HP15_39 0.49 +1.0 43,946 + HP15_39 0.49 -0.2 43,951 + HP15_39 0.49 +1.0 43,951 + HP15_39 0.49 -0.2 43,951 + HP15_39 0.49 +1.5 43,954 - HP15_39 0.50 +0.4 43,959 - HP15_39 0.50 -2.0 43,959 - HP15_39 0.50 +0.7 43,959 - HP15_39 0.50 -1.0 43,959 - HP15_39 0.50 +0.9 43,973 + HP15_39 0.51 +0.7 43,973 + HP15_39 0.51 -1.6 43,990 + HP15_39 0.52 -0.7 43,990 + HP15_39 0.52 +0.8 43,990 + HP15_39 0.52 +0.6 43,998 - HP15_39 0.53 -1.1 43,998 - HP15_39 0.53 +0.1 43,998 - HP15_39 0.53 +0.7 44,007 + HP15_39 0.54 -1.8 44,012 + HP15_39 0.54 +0.5 44,091 + HP15_39 0.60 +0.7 44,152 - HP15_39 0.65 +0.4 44,164 + HP15_39 0.66 +0.6 44,165 - HP15_39 0.66 +1.1 44,179 - HP15_39 0.67 -0.6 44,214 + HP15_39 0.69 +0.9 44,254 - HP15_39 0.72 +0.2 44,274 + HP15_39 0.74 -4.1 44,276 + HP15_39 0.74 +0.8 44,282 - HP15_39 0.75 +0.3 44,284 - HP15_39 0.75 +2.1 44,284 - HP15_39 0.75 -0.9 44,284 - HP15_39 0.75 -0.9 44,284 - HP15_39 0.75 -1.5 44,377 + HP15_39 0.82 -0.1 44,401 - HP15_39 0.84 +0.7 44,412 + HP15_39 0.84 +1.1 44,412 + HP15_39 0.84 -0.5 44,420 - HP15_39 0.85 +1.0 44,434 + HP15_39 0.86 -0.2 44,604 - +0.5 44,611 + -0.5 44,633 + -0.8 44,661 - +0.5 44,709 + +0.4 44,709 + -0.4 44,709 + -0.6 44,716 - +0.5 44,717 - +0.5 44,807 + +1.3 44,825 + -1.3 44,887 - -1.0 44,904 + +0.5 44,909 + -2.0 44,912 - -0.2 45,091 - HP15_40 0.27 +1.1 45,101 - HP15_40 0.33 +1.1 45,142 - HP15_40 0.58 -1.9 45,143 - HP15_40 0.58 -0.3 45,308 - +1.1 45,473 - HP15_41 0.30 -1.6 45,568 + HP15_41 0.57 -1.6 45,577 - HP15_41 0.60 -0.3 45,587 - HP15_41 0.62 +0.4
Or see this region's nucleotide sequence