Experiment: No vitamins, second passage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3057 and HP15_3058 are separated by 6 nucleotides HP15_3058 and HP15_3059 are separated by 9 nucleotides HP15_3059 and HP15_3060 are separated by 238 nucleotides HP15_3060 and HP15_3061 are separated by 74 nucleotides
HP15_3057: HP15_3057 - leucine/isoleucine/valine transporter permease subunit, at 3,207,965 to 3,209,236
_3057
HP15_3058: HP15_3058 - leucine/isoleucine/valine transporter ATP-binding subunit, at 3,209,243 to 3,209,998
_3058
HP15_3059: HP15_3059 - high-affinity branched-chain amino acid ABC transporter, ATP-binding protein, at 3,210,008 to 3,210,709
_3059
HP15_3060: HP15_3060 - conserved hypothetical protein, secreted, at 3,210,948 to 3,211,412
_3060
HP15_3061: HP15_3061 - conserved hypothetical protein, secreted, at 3,211,487 to 3,213,367
_3061
Position (kb)
3210
3211 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3209.016 kb on - strand, within HP15_3057 at 3209.148 kb on - strand at 3209.178 kb on - strand at 3209.178 kb on - strand at 3209.484 kb on + strand, within HP15_3058 at 3209.631 kb on - strand, within HP15_3058 at 3209.649 kb on + strand, within HP15_3058 at 3209.649 kb on + strand, within HP15_3058 at 3209.719 kb on - strand, within HP15_3058 at 3209.868 kb on + strand, within HP15_3058 at 3209.868 kb on + strand, within HP15_3058 at 3209.992 kb on + strand at 3210.000 kb on - strand at 3210.007 kb on - strand at 3210.025 kb on + strand at 3210.053 kb on + strand at 3210.136 kb on + strand, within HP15_3059 at 3210.218 kb on + strand, within HP15_3059 at 3210.266 kb on - strand, within HP15_3059 at 3210.380 kb on - strand, within HP15_3059 at 3210.459 kb on + strand, within HP15_3059 at 3210.820 kb on - strand at 3210.824 kb on + strand at 3211.059 kb on - strand, within HP15_3060 at 3211.165 kb on - strand, within HP15_3060 at 3211.165 kb on - strand, within HP15_3060 at 3211.205 kb on - strand, within HP15_3060 at 3211.245 kb on - strand, within HP15_3060 at 3211.245 kb on - strand, within HP15_3060 at 3211.419 kb on - strand at 3211.419 kb on - strand at 3211.483 kb on - strand at 3211.529 kb on + strand at 3211.537 kb on - strand at 3211.542 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins, second passage remove 3,209,016 - HP15_3057 0.83 -0.8 3,209,148 - +0.7 3,209,178 - +0.8 3,209,178 - +3.1 3,209,484 + HP15_3058 0.32 +1.5 3,209,631 - HP15_3058 0.51 -1.5 3,209,649 + HP15_3058 0.54 -1.4 3,209,649 + HP15_3058 0.54 +1.5 3,209,719 - HP15_3058 0.63 +0.7 3,209,868 + HP15_3058 0.83 +0.6 3,209,868 + HP15_3058 0.83 +0.0 3,209,992 + +0.1 3,210,000 - -2.1 3,210,007 - -0.1 3,210,025 + +0.5 3,210,053 + +1.1 3,210,136 + HP15_3059 0.18 -1.2 3,210,218 + HP15_3059 0.30 -0.7 3,210,266 - HP15_3059 0.37 -0.1 3,210,380 - HP15_3059 0.53 -1.3 3,210,459 + HP15_3059 0.64 -0.9 3,210,820 - -0.4 3,210,824 + +0.7 3,211,059 - HP15_3060 0.24 +0.3 3,211,165 - HP15_3060 0.47 +0.9 3,211,165 - HP15_3060 0.47 -0.6 3,211,205 - HP15_3060 0.55 +0.9 3,211,245 - HP15_3060 0.64 +0.7 3,211,245 - HP15_3060 0.64 -0.5 3,211,419 - -0.3 3,211,419 - +0.3 3,211,483 - +0.3 3,211,529 + +0.5 3,211,537 - -1.7 3,211,542 - +0.9
Or see this region's nucleotide sequence