Experiment: No vitamins, second passage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_259 and HP15_261 are separated by 101 nucleotides HP15_261 and HP15_260 are separated by 18 nucleotides HP15_260 and HP15_262 are separated by 101 nucleotides
HP15_259: HP15_259 - AraC-type DNA-binding domain-containing protein, at 249,773 to 250,849
_259
HP15_261: HP15_261 - hypothetical protein, at 250,951 to 251,220
_261
HP15_260: HP15_260 - hydrolase, alpha/beta fold family protein, at 251,239 to 252,036
_260
HP15_262: HP15_262 - strictosidine synthase family protein, at 252,138 to 253,223
_262
Position (kb) 250
251
252 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 249.954 kb on - strand, within HP15_259 at 250.006 kb on - strand, within HP15_259 at 250.033 kb on - strand, within HP15_259 at 250.052 kb on - strand, within HP15_259 at 250.057 kb on + strand, within HP15_259 at 250.161 kb on + strand, within HP15_259 at 250.190 kb on - strand, within HP15_259 at 250.397 kb on + strand, within HP15_259 at 250.405 kb on - strand, within HP15_259 at 250.528 kb on + strand, within HP15_259 at 250.554 kb on - strand, within HP15_259 at 250.808 kb on + strand at 250.813 kb on + strand at 250.816 kb on - strand at 250.816 kb on - strand at 250.831 kb on - strand at 250.876 kb on - strand at 250.911 kb on + strand at 251.187 kb on + strand, within HP15_261 at 251.195 kb on - strand at 251.210 kb on + strand at 251.235 kb on + strand at 251.311 kb on + strand at 251.322 kb on + strand, within HP15_260 at 251.575 kb on - strand, within HP15_260 at 251.605 kb on + strand, within HP15_260 at 251.624 kb on + strand, within HP15_260 at 251.629 kb on + strand, within HP15_260 at 251.637 kb on - strand, within HP15_260 at 251.670 kb on + strand, within HP15_260 at 251.832 kb on + strand, within HP15_260 at 251.832 kb on + strand, within HP15_260 at 251.941 kb on - strand, within HP15_260 at 251.965 kb on - strand at 251.993 kb on - strand at 252.044 kb on - strand at 252.044 kb on - strand at 252.085 kb on - strand at 252.179 kb on + strand at 252.187 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins, second passage remove 249,954 - HP15_259 0.17 +0.3 250,006 - HP15_259 0.22 +0.5 250,033 - HP15_259 0.24 +0.1 250,052 - HP15_259 0.26 -1.1 250,057 + HP15_259 0.26 +0.5 250,161 + HP15_259 0.36 +0.7 250,190 - HP15_259 0.39 -0.5 250,397 + HP15_259 0.58 +0.6 250,405 - HP15_259 0.59 -0.3 250,528 + HP15_259 0.70 -1.0 250,554 - HP15_259 0.73 +0.5 250,808 + +0.6 250,813 + +0.7 250,816 - +0.6 250,816 - -1.0 250,831 - +0.2 250,876 - -0.2 250,911 + +0.3 251,187 + HP15_261 0.87 -0.5 251,195 - +0.3 251,210 + -0.1 251,235 + +0.4 251,311 + -1.7 251,322 + HP15_260 0.10 -2.9 251,575 - HP15_260 0.42 -1.2 251,605 + HP15_260 0.46 +0.1 251,624 + HP15_260 0.48 +0.5 251,629 + HP15_260 0.49 -2.0 251,637 - HP15_260 0.50 -2.5 251,670 + HP15_260 0.54 -2.0 251,832 + HP15_260 0.74 -1.1 251,832 + HP15_260 0.74 -0.2 251,941 - HP15_260 0.88 -2.2 251,965 - -1.0 251,993 - -1.9 252,044 - -0.7 252,044 - -0.5 252,085 - +0.3 252,179 + -0.2 252,187 - +0.0
Or see this region's nucleotide sequence