Experiment: No vitamins, second passage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2170 and HP15_2171 are separated by 51 nucleotides HP15_2171 and HP15_2172 are separated by 251 nucleotides HP15_2172 and HP15_2173 are separated by 21 nucleotides HP15_2173 and HP15_2174 overlap by 4 nucleotides
HP15_2170: HP15_2170 - ABC-type sugar transport system, periplasmic component, at 2,279,786 to 2,281,570
_2170
HP15_2171: HP15_2171 - uracil-DNA glycosylase superfamily, at 2,281,622 to 2,282,071
_2171
HP15_2172: HP15_2172 - nitrorecductase, at 2,282,323 to 2,283,063
_2172
HP15_2173: HP15_2173 - glucokinase, at 2,283,085 to 2,284,050
_2173
HP15_2174: HP15_2174 - 6-phosphogluconate dehydratase, at 2,284,047 to 2,285,879
_2174
Position (kb)
2282
2283
2284 Strain fitness (log2 ratio)
-1
0
1 at 2281.366 kb on + strand, within HP15_2170 at 2281.366 kb on + strand, within HP15_2170 at 2281.374 kb on - strand, within HP15_2170 at 2281.374 kb on - strand, within HP15_2170 at 2281.379 kb on - strand, within HP15_2170 at 2281.547 kb on + strand at 2281.738 kb on - strand, within HP15_2171 at 2281.739 kb on - strand, within HP15_2171 at 2281.755 kb on + strand, within HP15_2171 at 2281.758 kb on + strand, within HP15_2171 at 2281.806 kb on + strand, within HP15_2171 at 2282.095 kb on - strand at 2282.226 kb on + strand at 2282.438 kb on - strand, within HP15_2172 at 2282.555 kb on + strand, within HP15_2172 at 2282.737 kb on + strand, within HP15_2172 at 2282.745 kb on - strand, within HP15_2172 at 2282.745 kb on - strand, within HP15_2172 at 2282.794 kb on - strand, within HP15_2172 at 2282.849 kb on - strand, within HP15_2172 at 2282.923 kb on - strand, within HP15_2172 at 2282.962 kb on + strand, within HP15_2172 at 2283.029 kb on + strand at 2283.057 kb on + strand at 2283.108 kb on - strand at 2283.249 kb on + strand, within HP15_2173 at 2283.297 kb on - strand, within HP15_2173 at 2283.367 kb on + strand, within HP15_2173 at 2283.422 kb on + strand, within HP15_2173 at 2283.422 kb on + strand, within HP15_2173 at 2283.460 kb on + strand, within HP15_2173 at 2283.468 kb on - strand, within HP15_2173 at 2283.575 kb on + strand, within HP15_2173 at 2283.593 kb on - strand, within HP15_2173 at 2283.649 kb on - strand, within HP15_2173 at 2283.742 kb on + strand, within HP15_2173 at 2283.922 kb on - strand, within HP15_2173 at 2284.014 kb on - strand at 2284.036 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins, second passage remove 2,281,366 + HP15_2170 0.89 -0.2 2,281,366 + HP15_2170 0.89 +0.5 2,281,374 - HP15_2170 0.89 -0.2 2,281,374 - HP15_2170 0.89 +0.9 2,281,379 - HP15_2170 0.89 -0.5 2,281,547 + -1.6 2,281,738 - HP15_2171 0.26 -0.4 2,281,739 - HP15_2171 0.26 +0.5 2,281,755 + HP15_2171 0.30 -0.6 2,281,758 + HP15_2171 0.30 +1.0 2,281,806 + HP15_2171 0.41 +0.6 2,282,095 - +0.6 2,282,226 + -0.1 2,282,438 - HP15_2172 0.16 -0.7 2,282,555 + HP15_2172 0.31 +1.2 2,282,737 + HP15_2172 0.56 +0.4 2,282,745 - HP15_2172 0.57 +0.4 2,282,745 - HP15_2172 0.57 -0.7 2,282,794 - HP15_2172 0.64 -0.2 2,282,849 - HP15_2172 0.71 -0.4 2,282,923 - HP15_2172 0.81 -0.4 2,282,962 + HP15_2172 0.86 -0.6 2,283,029 + +1.4 2,283,057 + +0.6 2,283,108 - -0.7 2,283,249 + HP15_2173 0.17 +1.1 2,283,297 - HP15_2173 0.22 -0.5 2,283,367 + HP15_2173 0.29 -0.6 2,283,422 + HP15_2173 0.35 +0.9 2,283,422 + HP15_2173 0.35 -0.4 2,283,460 + HP15_2173 0.39 -0.6 2,283,468 - HP15_2173 0.40 +0.7 2,283,575 + HP15_2173 0.51 +0.5 2,283,593 - HP15_2173 0.53 -0.6 2,283,649 - HP15_2173 0.58 -0.6 2,283,742 + HP15_2173 0.68 +0.1 2,283,922 - HP15_2173 0.87 -0.7 2,284,014 - +1.2 2,284,036 + +0.2
Or see this region's nucleotide sequence