Experiment: No vitamins, second passage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2042 and HP15_2043 are separated by 18 nucleotides HP15_2043 and HP15_2044 are separated by 25 nucleotides HP15_2044 and HP15_2045 are separated by 208 nucleotides HP15_2045 and HP15_2046 are separated by 13 nucleotides
HP15_2042: HP15_2042 - crossover junction endodeoxyribonuclease RuvC, at 2,141,033 to 2,141,560
_2042
HP15_2043: HP15_2043 - protein YebC, at 2,141,579 to 2,142,322
_2043
HP15_2044: HP15_2044 - aspartyl-tRNA synthetase, at 2,142,348 to 2,144,156
_2044
HP15_2045: HP15_2045 - DNA-binding transcriptional repressor FabR, at 2,144,365 to 2,144,982
_2045
HP15_2046: HP15_2046 - histidine triad (HIT) protein, at 2,144,996 to 2,145,436
_2046
Position (kb)
2142
2143
2144
2145 Strain fitness (log2 ratio)
-1
0
1
2 at 2141.585 kb on - strand at 2141.736 kb on - strand, within HP15_2043 at 2141.874 kb on - strand, within HP15_2043 at 2141.903 kb on + strand, within HP15_2043 at 2142.056 kb on - strand, within HP15_2043 at 2142.219 kb on + strand, within HP15_2043 at 2142.408 kb on - strand at 2144.569 kb on + strand, within HP15_2045 at 2144.588 kb on - strand, within HP15_2045 at 2144.715 kb on + strand, within HP15_2045 at 2144.800 kb on + strand, within HP15_2045 at 2144.910 kb on + strand, within HP15_2045 at 2144.930 kb on - strand at 2144.964 kb on - strand at 2144.989 kb on - strand at 2145.099 kb on + strand, within HP15_2046 at 2145.100 kb on + strand, within HP15_2046 at 2145.128 kb on - strand, within HP15_2046 at 2145.128 kb on - strand, within HP15_2046
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins, second passage remove 2,141,585 - +1.6 2,141,736 - HP15_2043 0.21 +1.8 2,141,874 - HP15_2043 0.40 +0.4 2,141,903 + HP15_2043 0.44 +0.3 2,142,056 - HP15_2043 0.64 -0.1 2,142,219 + HP15_2043 0.86 +1.0 2,142,408 - -1.3 2,144,569 + HP15_2045 0.33 -0.6 2,144,588 - HP15_2045 0.36 -1.3 2,144,715 + HP15_2045 0.57 -0.2 2,144,800 + HP15_2045 0.70 +0.9 2,144,910 + HP15_2045 0.88 +1.2 2,144,930 - +0.2 2,144,964 - +1.4 2,144,989 - -0.2 2,145,099 + HP15_2046 0.23 +1.2 2,145,100 + HP15_2046 0.24 +0.7 2,145,128 - HP15_2046 0.30 +0.7 2,145,128 - HP15_2046 0.30 +0.9
Or see this region's nucleotide sequence