Experiment: No vitamins, second passage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1615 and HP15_1616 are separated by 130 nucleotides HP15_1616 and HP15_1618 are separated by 14 nucleotides HP15_1618 and HP15_1617 are separated by 138 nucleotides HP15_1617 and HP15_1619 overlap by 28 nucleotides
HP15_1615: HP15_1615 - TonB-dependent receptor, plug, at 1,711,756 to 1,713,906
_1615
HP15_1616: HP15_1616 - protein containing DnaJ domain, at 1,714,037 to 1,714,681
_1616
HP15_1618: HP15_1618 - uncharacterized conserved protein UCP026166, sodium bile acid symporter, at 1,714,696 to 1,715,451
_1618
HP15_1617: HP15_1617 - hypothetical protein, at 1,715,590 to 1,715,721
_1617
HP15_1619: HP15_1619 - GntR domain protein, at 1,715,694 to 1,716,371
_1619
Position (kb)
1714
1715
1716 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1713.843 kb on + strand at 1713.861 kb on - strand at 1713.945 kb on - strand at 1714.004 kb on - strand at 1714.062 kb on - strand at 1714.067 kb on - strand at 1714.210 kb on + strand, within HP15_1616 at 1714.219 kb on - strand, within HP15_1616 at 1714.243 kb on - strand, within HP15_1616 at 1714.306 kb on + strand, within HP15_1616 at 1714.306 kb on + strand, within HP15_1616 at 1714.314 kb on - strand, within HP15_1616 at 1714.354 kb on + strand, within HP15_1616 at 1714.517 kb on - strand, within HP15_1616 at 1714.529 kb on - strand, within HP15_1616 at 1714.663 kb on - strand at 1714.677 kb on + strand at 1714.720 kb on - strand at 1714.749 kb on - strand at 1714.765 kb on - strand at 1714.882 kb on + strand, within HP15_1618 at 1714.980 kb on + strand, within HP15_1618 at 1715.325 kb on + strand, within HP15_1618 at 1715.358 kb on - strand, within HP15_1618 at 1715.416 kb on - strand at 1715.518 kb on + strand at 1715.535 kb on + strand at 1715.600 kb on - strand at 1715.663 kb on + strand, within HP15_1617 at 1715.671 kb on - strand, within HP15_1617 at 1715.700 kb on - strand at 1715.927 kb on + strand, within HP15_1619 at 1715.969 kb on + strand, within HP15_1619 at 1716.133 kb on - strand, within HP15_1619 at 1716.246 kb on - strand, within HP15_1619 at 1716.291 kb on + strand, within HP15_1619
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins, second passage remove 1,713,843 + -1.4 1,713,861 - +0.5 1,713,945 - +2.3 1,714,004 - -1.9 1,714,062 - -0.3 1,714,067 - +0.0 1,714,210 + HP15_1616 0.27 -1.4 1,714,219 - HP15_1616 0.28 -0.6 1,714,243 - HP15_1616 0.32 -0.4 1,714,306 + HP15_1616 0.42 -0.5 1,714,306 + HP15_1616 0.42 -0.8 1,714,314 - HP15_1616 0.43 -0.6 1,714,354 + HP15_1616 0.49 -1.7 1,714,517 - HP15_1616 0.74 -0.0 1,714,529 - HP15_1616 0.76 -1.4 1,714,663 - -0.1 1,714,677 + +1.4 1,714,720 - +1.3 1,714,749 - +2.0 1,714,765 - +2.3 1,714,882 + HP15_1618 0.25 +1.7 1,714,980 + HP15_1618 0.38 +1.0 1,715,325 + HP15_1618 0.83 +1.3 1,715,358 - HP15_1618 0.88 +1.3 1,715,416 - +1.5 1,715,518 + +1.0 1,715,535 + +1.6 1,715,600 - +2.7 1,715,663 + HP15_1617 0.55 +1.7 1,715,671 - HP15_1617 0.61 +2.1 1,715,700 - -0.3 1,715,927 + HP15_1619 0.34 +2.1 1,715,969 + HP15_1619 0.41 +1.3 1,716,133 - HP15_1619 0.65 -0.4 1,716,246 - HP15_1619 0.81 +2.0 1,716,291 + HP15_1619 0.88 +2.8
Or see this region's nucleotide sequence