Experiment: No vitamins, second passage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1599 and HP15_1600 are separated by 123 nucleotides HP15_1600 and HP15_1601 are separated by 8 nucleotides HP15_1601 and HP15_1602 are separated by 166 nucleotides
HP15_1599: HP15_1599 - alkylglycerone-phosphate synthase, at 1,695,660 to 1,697,264
_1599
HP15_1600: HP15_1600 - transglutaminase-like cysteine peptidase-like protein, at 1,697,388 to 1,698,050
_1600
HP15_1601: HP15_1601 - general secretion pathway protein C-like protein, at 1,698,059 to 1,698,709
_1601
HP15_1602: HP15_1602 - type II secretion system protein E, at 1,698,876 to 1,700,384
_1602
Position (kb)
1698
1699 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1697.073 kb on + strand, within HP15_1599 at 1697.099 kb on - strand, within HP15_1599 at 1697.199 kb on + strand at 1697.199 kb on + strand at 1697.397 kb on + strand at 1697.423 kb on + strand at 1697.423 kb on + strand at 1697.433 kb on + strand at 1697.628 kb on - strand, within HP15_1600 at 1697.644 kb on - strand, within HP15_1600 at 1697.659 kb on + strand, within HP15_1600 at 1697.697 kb on + strand, within HP15_1600 at 1697.853 kb on + strand, within HP15_1600 at 1698.081 kb on + strand at 1698.089 kb on - strand at 1698.089 kb on - strand at 1698.091 kb on + strand at 1698.099 kb on - strand at 1698.122 kb on - strand at 1698.182 kb on - strand, within HP15_1601 at 1698.195 kb on + strand, within HP15_1601 at 1698.260 kb on - strand, within HP15_1601 at 1698.304 kb on - strand, within HP15_1601 at 1698.519 kb on - strand, within HP15_1601 at 1698.582 kb on + strand, within HP15_1601 at 1698.584 kb on + strand, within HP15_1601 at 1698.670 kb on - strand at 1698.783 kb on + strand at 1698.789 kb on + strand at 1698.803 kb on - strand at 1699.018 kb on - strand at 1699.084 kb on + strand, within HP15_1602 at 1699.092 kb on - strand, within HP15_1602 at 1699.255 kb on - strand, within HP15_1602 at 1699.403 kb on + strand, within HP15_1602 at 1699.467 kb on - strand, within HP15_1602 at 1699.544 kb on - strand, within HP15_1602
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins, second passage remove 1,697,073 + HP15_1599 0.88 +0.9 1,697,099 - HP15_1599 0.90 -1.0 1,697,199 + +0.9 1,697,199 + +0.3 1,697,397 + -0.4 1,697,423 + +0.9 1,697,423 + -0.4 1,697,433 + +0.0 1,697,628 - HP15_1600 0.36 -0.9 1,697,644 - HP15_1600 0.39 -0.1 1,697,659 + HP15_1600 0.41 +0.6 1,697,697 + HP15_1600 0.47 -1.0 1,697,853 + HP15_1600 0.70 +0.6 1,698,081 + -1.1 1,698,089 - +1.6 1,698,089 - +1.9 1,698,091 + +1.2 1,698,099 - -0.0 1,698,122 - +0.6 1,698,182 - HP15_1601 0.19 -0.2 1,698,195 + HP15_1601 0.21 -0.2 1,698,260 - HP15_1601 0.31 -0.4 1,698,304 - HP15_1601 0.38 +0.5 1,698,519 - HP15_1601 0.71 -0.8 1,698,582 + HP15_1601 0.80 -0.4 1,698,584 + HP15_1601 0.81 -0.2 1,698,670 - -0.8 1,698,783 + -0.1 1,698,789 + -0.5 1,698,803 - -0.3 1,699,018 - +1.5 1,699,084 + HP15_1602 0.14 +1.5 1,699,092 - HP15_1602 0.14 +0.6 1,699,255 - HP15_1602 0.25 +1.1 1,699,403 + HP15_1602 0.35 +3.9 1,699,467 - HP15_1602 0.39 +1.6 1,699,544 - HP15_1602 0.44 +0.4
Or see this region's nucleotide sequence