Experiment: No vitamins, second passage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1264 and HP15_1265 are separated by 47 nucleotides HP15_1265 and HP15_1266 are separated by 57 nucleotides HP15_1266 and HP15_1267 are separated by 43 nucleotides HP15_1267 and HP15_1268 are separated by 68 nucleotides
HP15_1264: HP15_1264 - type IV-A pilus assembly ATPase PilB, at 1,323,402 to 1,325,192
_1264
HP15_1265: HP15_1265 - conserved hypothetical protein, membrane, at 1,325,240 to 1,325,611
_1265
HP15_1266: HP15_1266 - flavodoxin/nitric oxide synthase, at 1,325,669 to 1,326,274
_1266
HP15_1267: HP15_1267 - arsenate reductase, at 1,326,318 to 1,326,671
_1267
HP15_1268: HP15_1268 - thioredoxin, at 1,326,740 to 1,327,207
_1268
Position (kb) 1325
1326
1327 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 1324.694 kb on + strand, within HP15_1264 at 1324.694 kb on + strand, within HP15_1264 at 1324.702 kb on - strand, within HP15_1264 at 1324.707 kb on - strand, within HP15_1264 at 1324.763 kb on + strand, within HP15_1264 at 1324.817 kb on + strand, within HP15_1264 at 1324.838 kb on - strand, within HP15_1264 at 1324.922 kb on + strand, within HP15_1264 at 1324.929 kb on + strand, within HP15_1264 at 1324.992 kb on + strand, within HP15_1264 at 1325.065 kb on + strand at 1325.154 kb on + strand at 1325.184 kb on + strand at 1325.192 kb on - strand at 1325.192 kb on - strand at 1325.243 kb on + strand at 1325.312 kb on + strand, within HP15_1265 at 1325.320 kb on - strand, within HP15_1265 at 1325.335 kb on + strand, within HP15_1265 at 1325.453 kb on + strand, within HP15_1265 at 1325.491 kb on - strand, within HP15_1265 at 1325.502 kb on + strand, within HP15_1265 at 1325.537 kb on - strand, within HP15_1265 at 1325.611 kb on - strand at 1325.633 kb on + strand at 1325.641 kb on - strand at 1325.696 kb on + strand at 1325.725 kb on + strand at 1325.776 kb on + strand, within HP15_1266 at 1325.783 kb on + strand, within HP15_1266 at 1325.783 kb on + strand, within HP15_1266 at 1325.824 kb on + strand, within HP15_1266 at 1325.836 kb on + strand, within HP15_1266 at 1325.859 kb on - strand, within HP15_1266 at 1325.975 kb on - strand, within HP15_1266 at 1326.219 kb on - strand at 1326.231 kb on - strand at 1326.323 kb on - strand at 1326.443 kb on + strand, within HP15_1267 at 1326.448 kb on + strand, within HP15_1267 at 1326.451 kb on - strand, within HP15_1267 at 1326.451 kb on - strand, within HP15_1267 at 1326.453 kb on + strand, within HP15_1267 at 1326.462 kb on - strand, within HP15_1267 at 1326.532 kb on + strand, within HP15_1267 at 1326.742 kb on + strand at 1326.758 kb on + strand at 1326.776 kb on - strand at 1326.972 kb on + strand, within HP15_1268 at 1327.049 kb on + strand, within HP15_1268 at 1327.071 kb on + strand, within HP15_1268
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins, second passage remove 1,324,694 + HP15_1264 0.72 +4.3 1,324,694 + HP15_1264 0.72 +1.1 1,324,702 - HP15_1264 0.73 +0.9 1,324,707 - HP15_1264 0.73 +0.9 1,324,763 + HP15_1264 0.76 +0.0 1,324,817 + HP15_1264 0.79 -0.5 1,324,838 - HP15_1264 0.80 +2.0 1,324,922 + HP15_1264 0.85 +0.9 1,324,929 + HP15_1264 0.85 -2.7 1,324,992 + HP15_1264 0.89 +0.4 1,325,065 + +0.4 1,325,154 + +1.7 1,325,184 + +0.7 1,325,192 - -0.1 1,325,192 - -1.0 1,325,243 + -0.3 1,325,312 + HP15_1265 0.19 +0.3 1,325,320 - HP15_1265 0.22 +1.7 1,325,335 + HP15_1265 0.26 +1.0 1,325,453 + HP15_1265 0.57 +0.9 1,325,491 - HP15_1265 0.67 +0.7 1,325,502 + HP15_1265 0.70 +2.3 1,325,537 - HP15_1265 0.80 +1.7 1,325,611 - +0.1 1,325,633 + +0.3 1,325,641 - -0.0 1,325,696 + -0.8 1,325,725 + +0.8 1,325,776 + HP15_1266 0.18 -0.4 1,325,783 + HP15_1266 0.19 -0.1 1,325,783 + HP15_1266 0.19 -0.2 1,325,824 + HP15_1266 0.26 +1.3 1,325,836 + HP15_1266 0.28 -1.0 1,325,859 - HP15_1266 0.31 -1.2 1,325,975 - HP15_1266 0.50 -0.3 1,326,219 - +0.3 1,326,231 - -1.6 1,326,323 - -1.0 1,326,443 + HP15_1267 0.35 +1.2 1,326,448 + HP15_1267 0.37 +0.4 1,326,451 - HP15_1267 0.38 -0.4 1,326,451 - HP15_1267 0.38 +0.2 1,326,453 + HP15_1267 0.38 +1.0 1,326,462 - HP15_1267 0.41 -0.6 1,326,532 + HP15_1267 0.60 -0.1 1,326,742 + +0.1 1,326,758 + +1.2 1,326,776 - +0.7 1,326,972 + HP15_1268 0.50 +0.1 1,327,049 + HP15_1268 0.66 -0.9 1,327,071 + HP15_1268 0.71 -0.3
Or see this region's nucleotide sequence