Experiment: No vitamins, second passage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1233 and HP15_1234 are separated by 188 nucleotides HP15_1234 and HP15_1235 are separated by 13 nucleotides HP15_1235 and HP15_1236 overlap by 4 nucleotides
HP15_1233: HP15_1233 - acetyl-CoA acetyltransferase, at 1,283,888 to 1,285,096
_1233
HP15_1234: HP15_1234 - conserved hypothetical protein, at 1,285,285 to 1,285,548
_1234
HP15_1235: HP15_1235 - conserved hypothetical protein, at 1,285,562 to 1,285,900
_1235
HP15_1236: HP15_1236 - conserved hypothetical protein, at 1,285,897 to 1,288,026
_1236
Position (kb)
1285
1286 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1284.616 kb on + strand, within HP15_1233 at 1284.616 kb on - strand, within HP15_1233 at 1284.634 kb on + strand, within HP15_1233 at 1284.637 kb on - strand, within HP15_1233 at 1284.642 kb on - strand, within HP15_1233 at 1284.655 kb on - strand, within HP15_1233 at 1284.736 kb on + strand, within HP15_1233 at 1284.744 kb on - strand, within HP15_1233 at 1284.830 kb on + strand, within HP15_1233 at 1284.843 kb on - strand, within HP15_1233 at 1284.926 kb on + strand, within HP15_1233 at 1284.939 kb on + strand, within HP15_1233 at 1284.954 kb on + strand, within HP15_1233 at 1284.960 kb on + strand, within HP15_1233 at 1284.981 kb on + strand at 1284.981 kb on - strand at 1285.010 kb on + strand at 1285.059 kb on - strand at 1285.059 kb on - strand at 1285.068 kb on + strand at 1285.085 kb on - strand at 1285.179 kb on - strand at 1285.179 kb on - strand at 1285.188 kb on + strand at 1285.274 kb on - strand at 1285.346 kb on - strand, within HP15_1234 at 1285.357 kb on - strand, within HP15_1234 at 1285.407 kb on - strand, within HP15_1234 at 1285.407 kb on - strand, within HP15_1234 at 1285.468 kb on + strand, within HP15_1234 at 1285.542 kb on - strand at 1285.543 kb on + strand at 1285.632 kb on + strand, within HP15_1235 at 1285.694 kb on - strand, within HP15_1235 at 1285.774 kb on - strand, within HP15_1235 at 1285.845 kb on + strand, within HP15_1235 at 1285.845 kb on + strand, within HP15_1235 at 1285.850 kb on + strand, within HP15_1235 at 1285.853 kb on - strand, within HP15_1235 at 1285.853 kb on - strand, within HP15_1235 at 1285.858 kb on - strand, within HP15_1235 at 1285.940 kb on + strand at 1285.994 kb on + strand at 1286.120 kb on - strand, within HP15_1236 at 1286.269 kb on - strand, within HP15_1236 at 1286.269 kb on - strand, within HP15_1236 at 1286.308 kb on + strand, within HP15_1236 at 1286.372 kb on - strand, within HP15_1236 at 1286.384 kb on + strand, within HP15_1236 at 1286.394 kb on + strand, within HP15_1236 at 1286.436 kb on + strand, within HP15_1236 at 1286.449 kb on - strand, within HP15_1236 at 1286.449 kb on - strand, within HP15_1236 at 1286.449 kb on - strand, within HP15_1236 at 1286.873 kb on + strand, within HP15_1236 at 1286.883 kb on + strand, within HP15_1236
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins, second passage remove 1,284,616 + HP15_1233 0.60 +0.9 1,284,616 - HP15_1233 0.60 -0.5 1,284,634 + HP15_1233 0.62 +1.2 1,284,637 - HP15_1233 0.62 +0.8 1,284,642 - HP15_1233 0.62 +0.4 1,284,655 - HP15_1233 0.63 +0.2 1,284,736 + HP15_1233 0.70 +1.3 1,284,744 - HP15_1233 0.71 +1.8 1,284,830 + HP15_1233 0.78 +1.6 1,284,843 - HP15_1233 0.79 +0.3 1,284,926 + HP15_1233 0.86 -0.8 1,284,939 + HP15_1233 0.87 -1.6 1,284,954 + HP15_1233 0.88 +1.9 1,284,960 + HP15_1233 0.89 -0.3 1,284,981 + +1.1 1,284,981 - +0.5 1,285,010 + -0.1 1,285,059 - -0.5 1,285,059 - -1.1 1,285,068 + -1.5 1,285,085 - -0.2 1,285,179 - -0.5 1,285,179 - -1.2 1,285,188 + +1.0 1,285,274 - +1.1 1,285,346 - HP15_1234 0.23 -1.8 1,285,357 - HP15_1234 0.27 -0.9 1,285,407 - HP15_1234 0.46 -2.1 1,285,407 - HP15_1234 0.46 +0.2 1,285,468 + HP15_1234 0.69 +0.4 1,285,542 - -1.5 1,285,543 + +0.5 1,285,632 + HP15_1235 0.21 +0.8 1,285,694 - HP15_1235 0.39 +0.9 1,285,774 - HP15_1235 0.63 +1.5 1,285,845 + HP15_1235 0.83 +1.0 1,285,845 + HP15_1235 0.83 -0.7 1,285,850 + HP15_1235 0.85 +1.8 1,285,853 - HP15_1235 0.86 +0.0 1,285,853 - HP15_1235 0.86 -1.0 1,285,858 - HP15_1235 0.87 +0.6 1,285,940 + -0.2 1,285,994 + +0.5 1,286,120 - HP15_1236 0.10 +1.0 1,286,269 - HP15_1236 0.17 +0.2 1,286,269 - HP15_1236 0.17 +1.0 1,286,308 + HP15_1236 0.19 +1.3 1,286,372 - HP15_1236 0.22 +0.0 1,286,384 + HP15_1236 0.23 +1.0 1,286,394 + HP15_1236 0.23 +1.1 1,286,436 + HP15_1236 0.25 -1.5 1,286,449 - HP15_1236 0.26 +0.3 1,286,449 - HP15_1236 0.26 -0.1 1,286,449 - HP15_1236 0.26 +0.3 1,286,873 + HP15_1236 0.46 +0.6 1,286,883 + HP15_1236 0.46 +5.0
Or see this region's nucleotide sequence