Experiment: No vitamins, second passage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1030 and HP15_1031 are separated by 1 nucleotides HP15_1031 and HP15_1032 overlap by 4 nucleotides HP15_1032 and HP15_1033 are separated by 92 nucleotides
HP15_1030: HP15_1030 - sulfate permease family protein, at 1,048,090 to 1,049,577
_1030
HP15_1031: HP15_1031 - UspA domain protein, at 1,049,579 to 1,050,505
_1031
HP15_1032: HP15_1032 - phospholipase, patatin family, at 1,050,502 to 1,051,368
_1032
HP15_1033: HP15_1033 - flavoprotein, NADB Rossmann superfamily, at 1,051,461 to 1,052,642
_1033
Position (kb)
1049
1050
1051 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1048.593 kb on + strand, within HP15_1030 at 1048.606 kb on + strand, within HP15_1030 at 1048.644 kb on + strand, within HP15_1030 at 1048.734 kb on + strand, within HP15_1030 at 1048.766 kb on - strand, within HP15_1030 at 1048.784 kb on - strand, within HP15_1030 at 1048.846 kb on - strand, within HP15_1030 at 1048.848 kb on + strand, within HP15_1030 at 1048.867 kb on + strand, within HP15_1030 at 1048.867 kb on + strand, within HP15_1030 at 1048.867 kb on + strand, within HP15_1030 at 1048.875 kb on - strand, within HP15_1030 at 1048.875 kb on - strand, within HP15_1030 at 1048.875 kb on - strand, within HP15_1030 at 1048.907 kb on - strand, within HP15_1030 at 1049.180 kb on + strand, within HP15_1030 at 1049.195 kb on + strand, within HP15_1030 at 1049.208 kb on - strand, within HP15_1030 at 1049.232 kb on - strand, within HP15_1030 at 1049.314 kb on + strand, within HP15_1030 at 1049.439 kb on + strand at 1049.442 kb on - strand at 1049.447 kb on - strand at 1049.447 kb on - strand at 1049.447 kb on - strand at 1050.010 kb on - strand, within HP15_1031 at 1050.027 kb on - strand, within HP15_1031 at 1050.034 kb on - strand, within HP15_1031 at 1050.179 kb on + strand, within HP15_1031 at 1050.187 kb on - strand, within HP15_1031 at 1050.247 kb on + strand, within HP15_1031 at 1050.255 kb on - strand, within HP15_1031 at 1050.270 kb on + strand, within HP15_1031 at 1050.288 kb on - strand, within HP15_1031 at 1050.457 kb on - strand at 1050.530 kb on + strand at 1050.530 kb on + strand at 1050.530 kb on + strand at 1050.543 kb on - strand at 1050.683 kb on - strand, within HP15_1032 at 1050.790 kb on - strand, within HP15_1032 at 1050.824 kb on + strand, within HP15_1032 at 1050.883 kb on - strand, within HP15_1032 at 1050.921 kb on - strand, within HP15_1032 at 1050.934 kb on - strand, within HP15_1032 at 1050.966 kb on - strand, within HP15_1032 at 1050.987 kb on + strand, within HP15_1032 at 1051.000 kb on - strand, within HP15_1032 at 1051.039 kb on + strand, within HP15_1032 at 1051.120 kb on - strand, within HP15_1032 at 1051.253 kb on + strand, within HP15_1032 at 1051.261 kb on - strand, within HP15_1032 at 1051.276 kb on + strand, within HP15_1032 at 1051.285 kb on - strand at 1051.392 kb on - strand at 1051.401 kb on + strand at 1051.409 kb on - strand at 1051.420 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins, second passage remove 1,048,593 + HP15_1030 0.34 -0.1 1,048,606 + HP15_1030 0.35 -0.0 1,048,644 + HP15_1030 0.37 +0.1 1,048,734 + HP15_1030 0.43 +0.8 1,048,766 - HP15_1030 0.45 -2.7 1,048,784 - HP15_1030 0.47 -0.6 1,048,846 - HP15_1030 0.51 +1.0 1,048,848 + HP15_1030 0.51 -1.2 1,048,867 + HP15_1030 0.52 -2.5 1,048,867 + HP15_1030 0.52 -1.0 1,048,867 + HP15_1030 0.52 -0.7 1,048,875 - HP15_1030 0.53 +1.3 1,048,875 - HP15_1030 0.53 -0.1 1,048,875 - HP15_1030 0.53 +1.6 1,048,907 - HP15_1030 0.55 -0.8 1,049,180 + HP15_1030 0.73 +0.0 1,049,195 + HP15_1030 0.74 +0.1 1,049,208 - HP15_1030 0.75 +0.0 1,049,232 - HP15_1030 0.77 +0.2 1,049,314 + HP15_1030 0.82 -0.5 1,049,439 + -0.6 1,049,442 - -0.0 1,049,447 - +0.0 1,049,447 - +0.5 1,049,447 - -3.8 1,050,010 - HP15_1031 0.46 +0.4 1,050,027 - HP15_1031 0.48 -0.1 1,050,034 - HP15_1031 0.49 +0.7 1,050,179 + HP15_1031 0.65 +0.4 1,050,187 - HP15_1031 0.66 +1.0 1,050,247 + HP15_1031 0.72 +0.8 1,050,255 - HP15_1031 0.73 -0.4 1,050,270 + HP15_1031 0.75 -0.1 1,050,288 - HP15_1031 0.76 +0.3 1,050,457 - +1.8 1,050,530 + +0.5 1,050,530 + +0.4 1,050,530 + -0.3 1,050,543 - -0.5 1,050,683 - HP15_1032 0.21 +0.0 1,050,790 - HP15_1032 0.33 -1.3 1,050,824 + HP15_1032 0.37 +0.9 1,050,883 - HP15_1032 0.44 +3.0 1,050,921 - HP15_1032 0.48 -0.2 1,050,934 - HP15_1032 0.50 +0.2 1,050,966 - HP15_1032 0.54 +0.1 1,050,987 + HP15_1032 0.56 -0.8 1,051,000 - HP15_1032 0.57 -2.5 1,051,039 + HP15_1032 0.62 -0.6 1,051,120 - HP15_1032 0.71 +0.5 1,051,253 + HP15_1032 0.87 +0.4 1,051,261 - HP15_1032 0.88 -0.8 1,051,276 + HP15_1032 0.89 +0.2 1,051,285 - -1.2 1,051,392 - +0.3 1,051,401 + +0.4 1,051,409 - -0.5 1,051,420 - -0.1
Or see this region's nucleotide sequence