Experiment: No vitamins, second passage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_982 and HP15_983 are separated by 191 nucleotides HP15_983 and HP15_984 are separated by 94 nucleotides HP15_984 and HP15_985 are separated by 113 nucleotides HP15_985 and HP15_986 overlap by 4 nucleotides
HP15_982: HP15_982 - conserved hypothetical protein, membrane, at 990,304 to 993,279
_982
HP15_983: HP15_983 - glyoxalase family protein, at 993,471 to 993,974
_983
HP15_984: HP15_984 - transcriptional regulator, LysR family protein, at 994,069 to 994,938
_984
HP15_985: HP15_985 - glutathione-dependent formaldehyde-activating, GFA, at 995,052 to 995,402
_985
HP15_986: HP15_986 - beta-lactamase, at 995,399 to 996,613
_986
Position (kb) 994
995 Strain fitness (log2 ratio) -2
-1
0
1
2 at 993.092 kb on - strand at 993.128 kb on - strand at 993.177 kb on + strand at 993.177 kb on + strand at 993.220 kb on - strand at 993.242 kb on + strand at 993.250 kb on - strand at 993.254 kb on + strand at 993.490 kb on + strand at 993.540 kb on + strand, within HP15_983 at 993.766 kb on + strand, within HP15_983 at 993.792 kb on + strand, within HP15_983 at 993.792 kb on + strand, within HP15_983 at 993.800 kb on - strand, within HP15_983 at 993.800 kb on - strand, within HP15_983 at 993.800 kb on - strand, within HP15_983 at 993.807 kb on + strand, within HP15_983 at 993.815 kb on - strand, within HP15_983 at 993.820 kb on - strand, within HP15_983 at 993.820 kb on - strand, within HP15_983 at 993.891 kb on - strand, within HP15_983 at 993.891 kb on - strand, within HP15_983 at 993.895 kb on + strand, within HP15_983 at 993.914 kb on + strand, within HP15_983 at 993.959 kb on + strand at 993.978 kb on - strand at 993.980 kb on + strand at 993.985 kb on + strand at 993.985 kb on + strand at 993.993 kb on - strand at 994.014 kb on - strand at 994.296 kb on + strand, within HP15_984 at 994.296 kb on + strand, within HP15_984 at 994.367 kb on + strand, within HP15_984 at 994.375 kb on - strand, within HP15_984 at 994.388 kb on - strand, within HP15_984 at 994.533 kb on - strand, within HP15_984 at 994.571 kb on - strand, within HP15_984 at 994.690 kb on - strand, within HP15_984 at 994.754 kb on + strand, within HP15_984 at 994.800 kb on - strand, within HP15_984 at 994.818 kb on + strand, within HP15_984 at 994.818 kb on + strand, within HP15_984 at 994.818 kb on + strand, within HP15_984 at 994.818 kb on + strand, within HP15_984 at 994.826 kb on - strand, within HP15_984 at 994.826 kb on - strand, within HP15_984 at 994.826 kb on - strand, within HP15_984 at 994.850 kb on + strand, within HP15_984 at 995.100 kb on + strand, within HP15_985 at 995.199 kb on + strand, within HP15_985 at 995.246 kb on + strand, within HP15_985 at 995.337 kb on - strand, within HP15_985 at 995.416 kb on + strand at 995.438 kb on - strand at 995.456 kb on - strand at 995.478 kb on - strand at 995.518 kb on - strand at 995.690 kb on - strand, within HP15_986 at 995.713 kb on - strand, within HP15_986 at 995.813 kb on - strand, within HP15_986 at 995.900 kb on + strand, within HP15_986 at 995.916 kb on + strand, within HP15_986
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins, second passage remove 993,092 - +0.4 993,128 - -0.1 993,177 + +0.8 993,177 + -0.6 993,220 - -0.2 993,242 + +0.4 993,250 - -0.7 993,254 + +0.4 993,490 + -1.3 993,540 + HP15_983 0.14 +0.2 993,766 + HP15_983 0.59 +0.2 993,792 + HP15_983 0.64 +1.2 993,792 + HP15_983 0.64 -1.9 993,800 - HP15_983 0.65 -1.3 993,800 - HP15_983 0.65 +1.2 993,800 - HP15_983 0.65 +0.5 993,807 + HP15_983 0.67 -1.0 993,815 - HP15_983 0.68 +0.3 993,820 - HP15_983 0.69 -0.4 993,820 - HP15_983 0.69 -0.1 993,891 - HP15_983 0.83 +1.3 993,891 - HP15_983 0.83 +0.8 993,895 + HP15_983 0.84 -0.5 993,914 + HP15_983 0.88 -0.4 993,959 + -0.9 993,978 - +0.8 993,980 + +0.1 993,985 + +0.4 993,985 + +1.0 993,993 - +0.1 994,014 - -0.8 994,296 + HP15_984 0.26 +0.3 994,296 + HP15_984 0.26 +0.6 994,367 + HP15_984 0.34 -0.0 994,375 - HP15_984 0.35 -0.1 994,388 - HP15_984 0.37 +1.7 994,533 - HP15_984 0.53 +0.0 994,571 - HP15_984 0.58 +1.3 994,690 - HP15_984 0.71 +0.1 994,754 + HP15_984 0.79 +0.2 994,800 - HP15_984 0.84 +0.1 994,818 + HP15_984 0.86 -0.1 994,818 + HP15_984 0.86 +0.7 994,818 + HP15_984 0.86 +1.0 994,818 + HP15_984 0.86 +0.6 994,826 - HP15_984 0.87 -0.0 994,826 - HP15_984 0.87 +0.7 994,826 - HP15_984 0.87 +0.9 994,850 + HP15_984 0.90 +2.0 995,100 + HP15_985 0.14 +0.8 995,199 + HP15_985 0.42 -1.0 995,246 + HP15_985 0.55 +0.7 995,337 - HP15_985 0.81 +0.7 995,416 + +0.5 995,438 - +1.0 995,456 - -0.2 995,478 - +1.2 995,518 - -0.1 995,690 - HP15_986 0.24 +0.6 995,713 - HP15_986 0.26 -2.5 995,813 - HP15_986 0.34 +0.9 995,900 + HP15_986 0.41 +0.1 995,916 + HP15_986 0.43 +0.6
Or see this region's nucleotide sequence