Experiment: BHIS with Dimethyl Sulfoxide 0.2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4456 and BT4457 overlap by 4 nucleotides BT4457 and BT4458 are separated by 203 nucleotides BT4458 and BT4459 are separated by 80 nucleotides
BT4456: BT4456 - putative electron transport protein (NCBI ptt file), at 5,866,074 to 5,867,459
BT4456
BT4457: BT4457 - conserved hypothetical protein (NCBI ptt file), at 5,867,456 to 5,868,037
BT4457
BT4458: BT4458 - pyridoxine kinase (NCBI ptt file), at 5,868,241 to 5,869,113
BT4458
BT4459: BT4459 - conserved hypothetical protein, putative transport protein (NCBI ptt file), at 5,869,194 to 5,870,366
BT4459
Position (kb)
5868
5869
5870 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5867.246 kb on - strand, within BT4456 at 5867.347 kb on - strand at 5867.434 kb on + strand at 5867.435 kb on - strand at 5867.491 kb on + strand at 5867.491 kb on + strand at 5867.526 kb on + strand, within BT4457 at 5867.527 kb on - strand, within BT4457 at 5867.527 kb on - strand, within BT4457 at 5867.593 kb on - strand, within BT4457 at 5867.610 kb on + strand, within BT4457 at 5867.611 kb on - strand, within BT4457 at 5867.694 kb on + strand, within BT4457 at 5867.695 kb on - strand, within BT4457 at 5867.897 kb on + strand, within BT4457 at 5868.028 kb on - strand at 5868.087 kb on + strand at 5868.087 kb on + strand at 5868.123 kb on - strand at 5868.130 kb on - strand at 5868.173 kb on + strand at 5868.175 kb on + strand at 5868.177 kb on + strand at 5868.178 kb on - strand at 5868.178 kb on - strand at 5868.225 kb on - strand at 5868.225 kb on - strand at 5868.229 kb on - strand at 5868.231 kb on + strand at 5868.245 kb on - strand at 5868.253 kb on - strand at 5868.258 kb on - strand at 5868.384 kb on + strand, within BT4458 at 5868.478 kb on - strand, within BT4458 at 5868.481 kb on + strand, within BT4458 at 5868.564 kb on - strand, within BT4458 at 5868.745 kb on + strand, within BT4458 at 5868.746 kb on - strand, within BT4458 at 5868.746 kb on - strand, within BT4458 at 5868.786 kb on + strand, within BT4458 at 5868.787 kb on - strand, within BT4458 at 5868.787 kb on - strand, within BT4458 at 5868.830 kb on + strand, within BT4458 at 5868.831 kb on - strand, within BT4458 at 5868.832 kb on + strand, within BT4458 at 5868.833 kb on - strand, within BT4458 at 5868.839 kb on - strand, within BT4458 at 5868.839 kb on - strand, within BT4458 at 5868.912 kb on + strand, within BT4458 at 5869.027 kb on + strand at 5869.078 kb on - strand at 5869.086 kb on - strand at 5869.112 kb on - strand at 5869.112 kb on - strand at 5869.112 kb on - strand at 5869.169 kb on + strand at 5869.183 kb on - strand at 5869.234 kb on + strand at 5869.234 kb on + strand at 5869.234 kb on + strand at 5869.234 kb on + strand at 5869.271 kb on - strand at 5869.294 kb on - strand at 5869.295 kb on + strand at 5869.307 kb on - strand at 5869.345 kb on + strand, within BT4459 at 5869.345 kb on + strand, within BT4459 at 5869.349 kb on + strand, within BT4459 at 5869.349 kb on + strand, within BT4459 at 5869.350 kb on - strand, within BT4459 at 5869.350 kb on - strand, within BT4459 at 5869.354 kb on - strand, within BT4459 at 5869.354 kb on - strand, within BT4459 at 5869.395 kb on + strand, within BT4459 at 5869.396 kb on - strand, within BT4459 at 5869.396 kb on - strand, within BT4459 at 5869.400 kb on + strand, within BT4459 at 5869.431 kb on + strand, within BT4459 at 5869.432 kb on - strand, within BT4459 at 5869.607 kb on + strand, within BT4459 at 5869.636 kb on + strand, within BT4459 at 5869.636 kb on + strand, within BT4459 at 5869.650 kb on + strand, within BT4459 at 5869.650 kb on + strand, within BT4459 at 5869.671 kb on + strand, within BT4459 at 5869.672 kb on - strand, within BT4459 at 5869.693 kb on - strand, within BT4459 at 5869.697 kb on - strand, within BT4459 at 5869.697 kb on - strand, within BT4459 at 5869.697 kb on - strand, within BT4459 at 5869.716 kb on - strand, within BT4459 at 5869.722 kb on + strand, within BT4459 at 5869.723 kb on - strand, within BT4459 at 5869.723 kb on - strand, within BT4459 at 5869.803 kb on + strand, within BT4459 at 5869.803 kb on + strand, within BT4459 at 5869.804 kb on - strand, within BT4459 at 5869.804 kb on - strand, within BT4459 at 5869.804 kb on - strand, within BT4459 at 5869.822 kb on + strand, within BT4459 at 5869.890 kb on + strand, within BT4459 at 5869.895 kb on + strand, within BT4459 at 5869.968 kb on + strand, within BT4459 at 5870.025 kb on + strand, within BT4459 at 5870.026 kb on - strand, within BT4459 at 5870.027 kb on + strand, within BT4459 at 5870.112 kb on - strand, within BT4459 at 5870.112 kb on - strand, within BT4459
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 0.2 vol% remove 5,867,246 - BT4456 0.85 +0.1 5,867,347 - -0.6 5,867,434 + -1.1 5,867,435 - +1.7 5,867,491 + -2.2 5,867,491 + -2.9 5,867,526 + BT4457 0.12 +0.1 5,867,527 - BT4457 0.12 -0.7 5,867,527 - BT4457 0.12 -1.4 5,867,593 - BT4457 0.24 +0.1 5,867,610 + BT4457 0.26 -1.0 5,867,611 - BT4457 0.27 +1.5 5,867,694 + BT4457 0.41 -1.3 5,867,695 - BT4457 0.41 -1.9 5,867,897 + BT4457 0.76 -1.5 5,868,028 - +0.1 5,868,087 + +1.1 5,868,087 + -2.2 5,868,123 - -1.0 5,868,130 - -2.1 5,868,173 + +0.1 5,868,175 + +0.1 5,868,177 + -1.9 5,868,178 - -0.5 5,868,178 - -1.9 5,868,225 - -1.7 5,868,225 - +0.1 5,868,229 - -0.9 5,868,231 + -0.9 5,868,245 - -2.9 5,868,253 - -1.7 5,868,258 - -0.9 5,868,384 + BT4458 0.16 +0.1 5,868,478 - BT4458 0.27 +0.1 5,868,481 + BT4458 0.27 -1.9 5,868,564 - BT4458 0.37 -3.0 5,868,745 + BT4458 0.58 -2.0 5,868,746 - BT4458 0.58 -3.2 5,868,746 - BT4458 0.58 -0.9 5,868,786 + BT4458 0.62 -2.2 5,868,787 - BT4458 0.63 +0.1 5,868,787 - BT4458 0.63 -1.5 5,868,830 + BT4458 0.67 -0.9 5,868,831 - BT4458 0.68 -0.9 5,868,832 + BT4458 0.68 -2.2 5,868,833 - BT4458 0.68 -1.5 5,868,839 - BT4458 0.68 +0.1 5,868,839 - BT4458 0.68 -1.7 5,868,912 + BT4458 0.77 +0.1 5,869,027 + -2.5 5,869,078 - +0.1 5,869,086 - -1.3 5,869,112 - -1.4 5,869,112 - +1.7 5,869,112 - -0.6 5,869,169 + -0.9 5,869,183 - -2.0 5,869,234 + +0.1 5,869,234 + -2.3 5,869,234 + +0.5 5,869,234 + -0.9 5,869,271 - +0.2 5,869,294 - +0.1 5,869,295 + +0.1 5,869,307 - -0.2 5,869,345 + BT4459 0.13 +1.7 5,869,345 + BT4459 0.13 -0.9 5,869,349 + BT4459 0.13 -0.0 5,869,349 + BT4459 0.13 -2.7 5,869,350 - BT4459 0.13 -0.9 5,869,350 - BT4459 0.13 -0.9 5,869,354 - BT4459 0.14 -2.5 5,869,354 - BT4459 0.14 -0.5 5,869,395 + BT4459 0.17 +0.7 5,869,396 - BT4459 0.17 -1.3 5,869,396 - BT4459 0.17 -2.1 5,869,400 + BT4459 0.18 +0.1 5,869,431 + BT4459 0.20 +0.1 5,869,432 - BT4459 0.20 -1.3 5,869,607 + BT4459 0.35 -0.9 5,869,636 + BT4459 0.38 +0.7 5,869,636 + BT4459 0.38 -2.9 5,869,650 + BT4459 0.39 -2.7 5,869,650 + BT4459 0.39 -3.8 5,869,671 + BT4459 0.41 +1.1 5,869,672 - BT4459 0.41 -2.9 5,869,693 - BT4459 0.43 -1.2 5,869,697 - BT4459 0.43 -1.6 5,869,697 - BT4459 0.43 -2.5 5,869,697 - BT4459 0.43 -1.5 5,869,716 - BT4459 0.45 -0.9 5,869,722 + BT4459 0.45 -1.5 5,869,723 - BT4459 0.45 -1.8 5,869,723 - BT4459 0.45 -0.9 5,869,803 + BT4459 0.52 -0.9 5,869,803 + BT4459 0.52 -1.5 5,869,804 - BT4459 0.52 -1.5 5,869,804 - BT4459 0.52 -1.2 5,869,804 - BT4459 0.52 -1.0 5,869,822 + BT4459 0.54 -1.9 5,869,890 + BT4459 0.59 -3.8 5,869,895 + BT4459 0.60 -0.9 5,869,968 + BT4459 0.66 -1.7 5,870,025 + BT4459 0.71 -1.0 5,870,026 - BT4459 0.71 -1.6 5,870,027 + BT4459 0.71 -0.0 5,870,112 - BT4459 0.78 -2.2 5,870,112 - BT4459 0.78 +0.1
Or see this region's nucleotide sequence