Experiment: BHIS with Dimethyl Sulfoxide 0.2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4317 and BT4318 are separated by 42 nucleotides BT4318 and BT4319 are separated by 50 nucleotides BT4319 and BT4320 are separated by 25 nucleotides
BT4317: BT4317 - putative lipoprotein protein (NCBI ptt file), at 5,686,557 to 5,687,069
BT4317
BT4318: BT4318 - ATP-binding protein (NCBI ptt file), at 5,687,112 to 5,687,816
BT4318
BT4319: BT4319 - hypothetical protein (NCBI ptt file), at 5,687,867 to 5,689,357
BT4319
BT4320: BT4320 - putative zinc protease (NCBI ptt file), at 5,689,383 to 5,692,223
BT4320
Position (kb) 5687
5688
5689
5690 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5686.934 kb on + strand, within BT4317 at 5687.049 kb on + strand at 5687.101 kb on + strand at 5687.108 kb on - strand at 5687.121 kb on + strand at 5687.172 kb on - strand at 5687.220 kb on + strand, within BT4318 at 5687.226 kb on + strand, within BT4318 at 5687.406 kb on - strand, within BT4318 at 5687.424 kb on - strand, within BT4318 at 5687.540 kb on - strand, within BT4318 at 5687.558 kb on - strand, within BT4318 at 5687.563 kb on - strand, within BT4318 at 5687.563 kb on - strand, within BT4318 at 5687.825 kb on + strand at 5687.860 kb on - strand at 5688.034 kb on - strand, within BT4319 at 5688.141 kb on + strand, within BT4319 at 5688.172 kb on + strand, within BT4319 at 5688.186 kb on + strand, within BT4319 at 5688.224 kb on - strand, within BT4319 at 5688.279 kb on + strand, within BT4319 at 5688.279 kb on + strand, within BT4319 at 5688.284 kb on - strand, within BT4319 at 5688.332 kb on + strand, within BT4319 at 5688.405 kb on + strand, within BT4319 at 5688.405 kb on + strand, within BT4319 at 5688.415 kb on + strand, within BT4319 at 5688.422 kb on - strand, within BT4319 at 5688.553 kb on + strand, within BT4319 at 5688.556 kb on - strand, within BT4319 at 5688.556 kb on - strand, within BT4319 at 5688.680 kb on + strand, within BT4319 at 5688.681 kb on - strand, within BT4319 at 5688.690 kb on - strand, within BT4319 at 5688.733 kb on + strand, within BT4319 at 5688.914 kb on + strand, within BT4319 at 5688.929 kb on + strand, within BT4319 at 5688.930 kb on - strand, within BT4319 at 5688.961 kb on + strand, within BT4319 at 5688.961 kb on + strand, within BT4319 at 5688.985 kb on + strand, within BT4319 at 5688.986 kb on - strand, within BT4319 at 5689.166 kb on + strand, within BT4319 at 5689.173 kb on + strand, within BT4319 at 5689.196 kb on - strand, within BT4319 at 5689.353 kb on - strand at 5689.361 kb on - strand at 5689.369 kb on + strand at 5689.377 kb on - strand at 5689.405 kb on - strand at 5689.534 kb on + strand at 5689.599 kb on - strand at 5689.714 kb on - strand, within BT4320 at 5689.714 kb on - strand, within BT4320 at 5689.881 kb on - strand, within BT4320 at 5689.881 kb on - strand, within BT4320 at 5689.945 kb on + strand, within BT4320 at 5689.974 kb on - strand, within BT4320 at 5690.021 kb on - strand, within BT4320 at 5690.021 kb on - strand, within BT4320 at 5690.064 kb on + strand, within BT4320 at 5690.122 kb on - strand, within BT4320 at 5690.207 kb on + strand, within BT4320 at 5690.320 kb on + strand, within BT4320 at 5690.320 kb on + strand, within BT4320
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 0.2 vol% remove 5,686,934 + BT4317 0.73 -1.8 5,687,049 + -0.9 5,687,101 + +0.1 5,687,108 - -2.5 5,687,121 + -1.4 5,687,172 - -0.9 5,687,220 + BT4318 0.15 +0.1 5,687,226 + BT4318 0.16 -2.5 5,687,406 - BT4318 0.42 -0.9 5,687,424 - BT4318 0.44 -2.9 5,687,540 - BT4318 0.61 -1.3 5,687,558 - BT4318 0.63 -1.0 5,687,563 - BT4318 0.64 -1.6 5,687,563 - BT4318 0.64 -1.5 5,687,825 + -3.0 5,687,860 - -2.3 5,688,034 - BT4319 0.11 -3.5 5,688,141 + BT4319 0.18 -2.0 5,688,172 + BT4319 0.20 -2.2 5,688,186 + BT4319 0.21 -2.2 5,688,224 - BT4319 0.24 -2.4 5,688,279 + BT4319 0.28 -2.5 5,688,279 + BT4319 0.28 -1.1 5,688,284 - BT4319 0.28 +0.1 5,688,332 + BT4319 0.31 +0.1 5,688,405 + BT4319 0.36 -1.2 5,688,405 + BT4319 0.36 -1.9 5,688,415 + BT4319 0.37 -1.9 5,688,422 - BT4319 0.37 -1.5 5,688,553 + BT4319 0.46 -2.1 5,688,556 - BT4319 0.46 +0.1 5,688,556 - BT4319 0.46 -2.4 5,688,680 + BT4319 0.55 +0.1 5,688,681 - BT4319 0.55 -1.9 5,688,690 - BT4319 0.55 -0.3 5,688,733 + BT4319 0.58 -0.2 5,688,914 + BT4319 0.70 +1.1 5,688,929 + BT4319 0.71 -1.5 5,688,930 - BT4319 0.71 -1.9 5,688,961 + BT4319 0.73 -1.9 5,688,961 + BT4319 0.73 +0.5 5,688,985 + BT4319 0.75 -3.0 5,688,986 - BT4319 0.75 -1.5 5,689,166 + BT4319 0.87 +0.7 5,689,173 + BT4319 0.88 -1.3 5,689,196 - BT4319 0.89 -2.7 5,689,353 - -1.9 5,689,361 - -2.5 5,689,369 + +0.1 5,689,377 - -1.9 5,689,405 - +0.1 5,689,534 + -2.2 5,689,599 - -1.2 5,689,714 - BT4320 0.12 -1.5 5,689,714 - BT4320 0.12 +0.1 5,689,881 - BT4320 0.18 -0.4 5,689,881 - BT4320 0.18 +0.1 5,689,945 + BT4320 0.20 +0.1 5,689,974 - BT4320 0.21 -2.5 5,690,021 - BT4320 0.22 -0.9 5,690,021 - BT4320 0.22 +0.5 5,690,064 + BT4320 0.24 -1.2 5,690,122 - BT4320 0.26 -1.7 5,690,207 + BT4320 0.29 +0.1 5,690,320 + BT4320 0.33 -1.9 5,690,320 + BT4320 0.33 +0.9
Or see this region's nucleotide sequence