Experiment: BHIS with Dimethyl Sulfoxide 0.2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4255 and BT4256 overlap by 4 nucleotides BT4256 and BT4257 overlap by 14 nucleotides BT4257 and BT4258 overlap by 56 nucleotides BT4258 and BT4259 overlap by 89 nucleotides
BT4255: BT4255 - DNA replication and repair protein RecF, ABC family ATPase (NCBI ptt file), at 5,606,265 to 5,607,374
BT4255
BT4256: BT4256 - conserved hypothetical protein (NCBI ptt file), at 5,607,371 to 5,607,658
BT4256
BT4257: BT4257 - putative histidinol-phosphatase (NCBI ptt file), at 5,607,645 to 5,608,436
BT4257
BT4258: BT4258 - putative 5-formyltetrahydrofolate cyclo-ligase (NCBI ptt file), at 5,608,381 to 5,608,971
BT4258
BT4259: BT4259 - carboxy-terminal processing protease precursor (NCBI ptt file), at 5,608,883 to 5,610,631
BT4259
Position (kb) 5607
5608
5609 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5606.732 kb on + strand, within BT4255 at 5606.753 kb on + strand, within BT4255 at 5606.753 kb on + strand, within BT4255 at 5607.029 kb on + strand, within BT4255 at 5607.044 kb on + strand, within BT4255 at 5607.182 kb on + strand, within BT4255 at 5607.260 kb on + strand, within BT4255 at 5607.269 kb on + strand at 5607.346 kb on + strand at 5607.724 kb on + strand at 5607.743 kb on - strand, within BT4257 at 5607.799 kb on - strand, within BT4257 at 5607.799 kb on - strand, within BT4257 at 5607.832 kb on - strand, within BT4257 at 5607.864 kb on + strand, within BT4257 at 5607.864 kb on + strand, within BT4257 at 5607.865 kb on - strand, within BT4257 at 5607.870 kb on - strand, within BT4257 at 5607.929 kb on + strand, within BT4257 at 5607.986 kb on - strand, within BT4257 at 5608.044 kb on - strand, within BT4257 at 5608.048 kb on - strand, within BT4257 at 5608.059 kb on - strand, within BT4257 at 5608.072 kb on + strand, within BT4257 at 5608.142 kb on + strand, within BT4257 at 5608.143 kb on - strand, within BT4257 at 5608.165 kb on + strand, within BT4257 at 5608.220 kb on - strand, within BT4257 at 5608.249 kb on - strand, within BT4257 at 5608.253 kb on - strand, within BT4257 at 5608.295 kb on - strand, within BT4257 at 5608.445 kb on - strand, within BT4258 at 5608.446 kb on + strand, within BT4258 at 5608.450 kb on + strand, within BT4258 at 5608.451 kb on - strand, within BT4258 at 5608.524 kb on + strand, within BT4258 at 5608.525 kb on - strand, within BT4258 at 5608.539 kb on + strand, within BT4258 at 5608.560 kb on - strand, within BT4258 at 5608.560 kb on - strand, within BT4258 at 5608.560 kb on - strand, within BT4258 at 5608.563 kb on - strand, within BT4258 at 5608.630 kb on + strand, within BT4258 at 5608.630 kb on + strand, within BT4258 at 5608.713 kb on - strand, within BT4258 at 5608.721 kb on - strand, within BT4258 at 5608.744 kb on + strand, within BT4258 at 5608.745 kb on - strand, within BT4258 at 5608.831 kb on + strand, within BT4258 at 5608.831 kb on + strand, within BT4258 at 5608.894 kb on + strand at 5608.941 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 0.2 vol% remove 5,606,732 + BT4255 0.42 -0.9 5,606,753 + BT4255 0.44 +0.4 5,606,753 + BT4255 0.44 -1.5 5,607,029 + BT4255 0.69 -0.4 5,607,044 + BT4255 0.70 -0.5 5,607,182 + BT4255 0.83 -1.9 5,607,260 + BT4255 0.90 +0.1 5,607,269 + -0.5 5,607,346 + +0.1 5,607,724 + -1.5 5,607,743 - BT4257 0.12 -0.9 5,607,799 - BT4257 0.19 -1.0 5,607,799 - BT4257 0.19 -1.6 5,607,832 - BT4257 0.24 +0.1 5,607,864 + BT4257 0.28 -0.9 5,607,864 + BT4257 0.28 -2.5 5,607,865 - BT4257 0.28 +0.1 5,607,870 - BT4257 0.28 -1.6 5,607,929 + BT4257 0.36 -2.2 5,607,986 - BT4257 0.43 -0.9 5,608,044 - BT4257 0.50 -2.1 5,608,048 - BT4257 0.51 -2.0 5,608,059 - BT4257 0.52 +1.1 5,608,072 + BT4257 0.54 -1.5 5,608,142 + BT4257 0.63 -1.8 5,608,143 - BT4257 0.63 -2.7 5,608,165 + BT4257 0.66 -1.5 5,608,220 - BT4257 0.73 +0.1 5,608,249 - BT4257 0.76 -2.2 5,608,253 - BT4257 0.77 -1.3 5,608,295 - BT4257 0.82 -1.8 5,608,445 - BT4258 0.11 -0.6 5,608,446 + BT4258 0.11 +0.6 5,608,450 + BT4258 0.12 -0.9 5,608,451 - BT4258 0.12 -0.4 5,608,524 + BT4258 0.24 +0.1 5,608,525 - BT4258 0.24 -1.6 5,608,539 + BT4258 0.27 +0.1 5,608,560 - BT4258 0.30 -0.9 5,608,560 - BT4258 0.30 -3.1 5,608,560 - BT4258 0.30 -2.5 5,608,563 - BT4258 0.31 -0.6 5,608,630 + BT4258 0.42 -1.0 5,608,630 + BT4258 0.42 -0.5 5,608,713 - BT4258 0.56 -1.1 5,608,721 - BT4258 0.58 -0.6 5,608,744 + BT4258 0.61 -0.9 5,608,745 - BT4258 0.62 +0.1 5,608,831 + BT4258 0.76 -0.9 5,608,831 + BT4258 0.76 -0.9 5,608,894 + -0.6 5,608,941 + +0.2
Or see this region's nucleotide sequence