Experiment: BHIS with Dimethyl Sulfoxide 0.2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0514 and BT0515 are separated by 702 nucleotides BT0515 and BT0516 are separated by 153 nucleotides BT0516 and BT0517 are separated by 33 nucleotides
BT0514: BT0514 - conserved hypothetical protein (NCBI ptt file), at 636,473 to 637,477
BT0514
BT0515: BT0515 - terminal quinol oxidase, subunit, putative (doxD-like) (NCBI ptt file), at 638,180 to 639,205
BT0515
BT0516: BT0516 - conserved hypothetical protein (NCBI ptt file), at 639,359 to 639,721
BT0516
BT0517: BT0517 - flavodoxin (NCBI ptt file), at 639,755 to 640,261
BT0517
Position (kb) 638
639
640 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 637.301 kb on + strand, within BT0514 at 637.302 kb on - strand, within BT0514 at 637.349 kb on + strand at 637.373 kb on - strand, within BT0514 at 637.571 kb on - strand at 637.581 kb on + strand at 637.696 kb on + strand at 637.804 kb on + strand at 637.805 kb on - strand at 637.822 kb on + strand at 637.896 kb on + strand at 638.021 kb on + strand at 638.062 kb on - strand at 638.066 kb on - strand at 638.067 kb on + strand at 638.074 kb on + strand at 638.219 kb on + strand at 638.220 kb on - strand at 638.285 kb on - strand, within BT0515 at 638.349 kb on + strand, within BT0515 at 638.461 kb on - strand, within BT0515 at 638.596 kb on + strand at 638.597 kb on - strand, within BT0515 at 638.599 kb on + strand, within BT0515 at 638.603 kb on + strand, within BT0515 at 638.687 kb on - strand, within BT0515 at 638.825 kb on - strand, within BT0515 at 638.882 kb on - strand, within BT0515 at 638.913 kb on + strand, within BT0515 at 638.914 kb on - strand, within BT0515 at 638.914 kb on - strand, within BT0515 at 638.937 kb on - strand, within BT0515 at 638.939 kb on + strand, within BT0515 at 638.940 kb on - strand, within BT0515 at 639.055 kb on - strand, within BT0515 at 639.167 kb on + strand at 639.338 kb on + strand at 639.374 kb on + strand at 639.375 kb on - strand at 639.397 kb on - strand, within BT0516 at 639.397 kb on - strand, within BT0516 at 639.490 kb on + strand, within BT0516 at 639.508 kb on + strand, within BT0516 at 639.509 kb on - strand, within BT0516 at 639.545 kb on - strand, within BT0516 at 639.628 kb on + strand, within BT0516 at 639.629 kb on - strand, within BT0516 at 639.629 kb on - strand, within BT0516 at 639.636 kb on - strand, within BT0516 at 639.667 kb on - strand, within BT0516 at 639.727 kb on - strand at 639.727 kb on - strand at 639.734 kb on + strand at 639.739 kb on + strand at 639.973 kb on + strand, within BT0517 at 639.974 kb on - strand, within BT0517 at 640.014 kb on - strand, within BT0517 at 640.014 kb on - strand, within BT0517 at 640.062 kb on + strand, within BT0517 at 640.184 kb on + strand, within BT0517
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 0.2 vol% remove 637,301 + BT0514 0.82 -0.8 637,302 - BT0514 0.82 +0.2 637,349 + +0.2 637,373 - BT0514 0.90 -0.8 637,571 - -2.4 637,581 + -0.8 637,696 + +0.2 637,804 + -2.4 637,805 - -2.0 637,822 + +0.2 637,896 + +1.2 638,021 + -2.8 638,062 - +0.2 638,066 - -0.2 638,067 + -0.8 638,074 + +0.2 638,219 + -1.4 638,220 - +0.2 638,285 - BT0515 0.10 +1.2 638,349 + BT0515 0.16 -1.0 638,461 - BT0515 0.27 -2.2 638,596 + +0.2 638,597 - BT0515 0.41 +0.2 638,599 + BT0515 0.41 +0.2 638,603 + BT0515 0.41 +0.5 638,687 - BT0515 0.49 -2.1 638,825 - BT0515 0.63 -2.0 638,882 - BT0515 0.68 -2.5 638,913 + BT0515 0.71 -2.1 638,914 - BT0515 0.72 -2.1 638,914 - BT0515 0.72 -0.4 638,937 - BT0515 0.74 -0.8 638,939 + BT0515 0.74 -2.0 638,940 - BT0515 0.74 -1.8 639,055 - BT0515 0.85 -1.8 639,167 + -1.4 639,338 + -2.4 639,374 + -2.1 639,375 - -0.8 639,397 - BT0516 0.10 -1.8 639,397 - BT0516 0.10 +0.2 639,490 + BT0516 0.36 +1.0 639,508 + BT0516 0.41 -0.8 639,509 - BT0516 0.41 -1.0 639,545 - BT0516 0.51 +0.2 639,628 + BT0516 0.74 -1.6 639,629 - BT0516 0.74 +0.2 639,629 - BT0516 0.74 +0.6 639,636 - BT0516 0.76 -2.1 639,667 - BT0516 0.85 -1.4 639,727 - +0.2 639,727 - -0.6 639,734 + +0.2 639,739 + -0.8 639,973 + BT0517 0.43 -2.8 639,974 - BT0517 0.43 -0.4 640,014 - BT0517 0.51 -2.4 640,014 - BT0517 0.51 +1.2 640,062 + BT0517 0.61 -0.7 640,184 + BT0517 0.85 +0.2
Or see this region's nucleotide sequence