Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS10395

Experiment: water survival rep A; Treatment=watercosm; Collection=outgrowth_in_CPG; Time=90_days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS10385 and RS_RS10390 are separated by 97 nucleotidesRS_RS10390 and RS_RS10395 are separated by 245 nucleotidesRS_RS10395 and RS_RS10400 are separated by 340 nucleotides RS_RS10385: RS_RS10385 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,242,576 to 2,243,832 _RS10385 RS_RS10390: RS_RS10390 - membrane protein, at 2,243,930 to 2,244,451 _RS10390 RS_RS10395: RS_RS10395 - 2-isopropylmalate synthase, at 2,244,697 to 2,246,238 _RS10395 RS_RS10400: RS_RS10400 - CDP-diacylglycerol--serine O-phosphatidyltransferase, at 2,246,579 to 2,247,454 _RS10400 Position (kb) 2244 2245 2246 2247Strain fitness (log2 ratio) -1 0 1 2 3at 2243.832 kb on + strandat 2243.832 kb on + strandat 2243.860 kb on + strandat 2243.861 kb on - strandat 2244.290 kb on + strand, within RS_RS10390at 2244.423 kb on - strandat 2245.305 kb on - strand, within RS_RS10395at 2245.305 kb on - strand, within RS_RS10395at 2245.308 kb on + strand, within RS_RS10395at 2245.701 kb on - strand, within RS_RS10395at 2245.883 kb on + strand, within RS_RS10395at 2245.921 kb on - strand, within RS_RS10395at 2245.921 kb on - strand, within RS_RS10395at 2246.413 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction water survival rep A; Treatment=watercosm; Collection=outgrowth_in_CPG; Time=90_days
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2,243,832 + -0.2
2,243,832 + +0.5
2,243,860 + +0.2
2,243,861 - +0.5
2,244,290 + RS_RS10390 0.69 +1.1
2,244,423 - -0.4
2,245,305 - RS_RS10395 0.39 +0.2
2,245,305 - RS_RS10395 0.39 +0.8
2,245,308 + RS_RS10395 0.40 -0.1
2,245,701 - RS_RS10395 0.65 -1.3
2,245,883 + RS_RS10395 0.77 +2.9
2,245,921 - RS_RS10395 0.79 +0.4
2,245,921 - RS_RS10395 0.79 +0.4
2,246,413 + -0.8

Or see this region's nucleotide sequence