Experiment: Taped volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS23430 and HSERO_RS23435 are separated by 167 nucleotides HSERO_RS23435 and HSERO_RS23440 are separated by 99 nucleotides HSERO_RS23440 and HSERO_RS23445 are separated by 51 nucleotides HSERO_RS23445 and HSERO_RS23450 overlap by 1 nucleotides
HSERO_RS23430: HSERO_RS23430 - Zn-dependent hydrolase, at 5,375,223 to 5,376,287
_RS23430
HSERO_RS23435: HSERO_RS23435 - MerR family transcriptional regulator, at 5,376,455 to 5,376,883
_RS23435
HSERO_RS23440: HSERO_RS23440 - isovaleryl-CoA dehydrogenase, at 5,376,983 to 5,378,167
_RS23440
HSERO_RS23445: HSERO_RS23445 - 2-hydroxychromene-2-carboxylate isomerase, at 5,378,219 to 5,378,821
_RS23445
HSERO_RS23450: HSERO_RS23450 - hypothetical protein, at 5,378,821 to 5,379,231
_RS23450
Position (kb) 5376
5377
5378
5379 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5376.185 kb on - strand at 5376.577 kb on + strand, within HSERO_RS23435 at 5376.701 kb on - strand, within HSERO_RS23435 at 5376.725 kb on + strand, within HSERO_RS23435 at 5376.980 kb on + strand at 5377.155 kb on + strand, within HSERO_RS23440 at 5377.263 kb on - strand, within HSERO_RS23440 at 5377.338 kb on + strand, within HSERO_RS23440 at 5377.738 kb on + strand, within HSERO_RS23440 at 5377.976 kb on + strand, within HSERO_RS23440 at 5378.161 kb on + strand at 5378.229 kb on - strand at 5378.389 kb on + strand, within HSERO_RS23445 at 5378.553 kb on - strand, within HSERO_RS23445 at 5379.046 kb on + strand, within HSERO_RS23450 at 5379.054 kb on - strand, within HSERO_RS23450
Per-strain Table
Position Strand Gene LocusTag Fraction Taped volatile agar plate with no fungus remove 5,376,185 - -0.3 5,376,577 + HSERO_RS23435 0.28 -0.1 5,376,701 - HSERO_RS23435 0.57 -2.8 5,376,725 + HSERO_RS23435 0.63 +0.8 5,376,980 + +1.9 5,377,155 + HSERO_RS23440 0.15 -2.0 5,377,263 - HSERO_RS23440 0.24 -1.0 5,377,338 + HSERO_RS23440 0.30 -0.6 5,377,738 + HSERO_RS23440 0.64 +0.4 5,377,976 + HSERO_RS23440 0.84 -1.6 5,378,161 + -0.0 5,378,229 - -0.8 5,378,389 + HSERO_RS23445 0.28 -2.0 5,378,553 - HSERO_RS23445 0.55 -0.6 5,379,046 + HSERO_RS23450 0.55 -0.1 5,379,054 - HSERO_RS23450 0.57 -1.5
Or see this region's nucleotide sequence