Experiment: Taped volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS16830 and HSERO_RS16835 overlap by 4 nucleotides HSERO_RS16835 and HSERO_RS16840 are separated by 133 nucleotides HSERO_RS16840 and HSERO_RS16845 are separated by 88 nucleotides
HSERO_RS16830: HSERO_RS16830 - thiamine monophosphate kinase, at 3,854,111 to 3,855,076
_RS16830
HSERO_RS16835: HSERO_RS16835 - phosphatidylglycerophosphatase, at 3,855,073 to 3,855,615
_RS16835
HSERO_RS16840: HSERO_RS16840 - competence damage-inducible protein A, at 3,855,749 to 3,856,231
_RS16840
HSERO_RS16845: HSERO_RS16845 - orotidine 5'-phosphate decarboxylase, at 3,856,320 to 3,857,138
_RS16845
Position (kb)
3855
3856 Strain fitness (log2 ratio)
-2
-1
0
1 at 3854.105 kb on - strand at 3855.234 kb on - strand, within HSERO_RS16835 at 3855.240 kb on + strand, within HSERO_RS16835 at 3855.248 kb on - strand, within HSERO_RS16835 at 3855.258 kb on - strand, within HSERO_RS16835 at 3855.544 kb on - strand, within HSERO_RS16835 at 3855.580 kb on - strand at 3855.759 kb on + strand at 3855.759 kb on + strand at 3855.817 kb on - strand, within HSERO_RS16840 at 3855.945 kb on + strand, within HSERO_RS16840 at 3856.122 kb on - strand, within HSERO_RS16840 at 3856.137 kb on - strand, within HSERO_RS16840 at 3856.150 kb on + strand, within HSERO_RS16840 at 3856.241 kb on + strand at 3856.254 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Taped volatile agar plate with no fungus remove 3,854,105 - +0.3 3,855,234 - HSERO_RS16835 0.30 -2.1 3,855,240 + HSERO_RS16835 0.31 -0.2 3,855,248 - HSERO_RS16835 0.32 +0.1 3,855,258 - HSERO_RS16835 0.34 -0.4 3,855,544 - HSERO_RS16835 0.87 -1.1 3,855,580 - -1.0 3,855,759 + +0.2 3,855,759 + +0.5 3,855,817 - HSERO_RS16840 0.14 -0.8 3,855,945 + HSERO_RS16840 0.41 -0.5 3,856,122 - HSERO_RS16840 0.77 -2.5 3,856,137 - HSERO_RS16840 0.80 -0.7 3,856,150 + HSERO_RS16840 0.83 -0.1 3,856,241 + +0.9 3,856,254 - +0.9
Or see this region's nucleotide sequence