Experiment: Taped volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS07275 and HSERO_RS07280 are separated by 192 nucleotides HSERO_RS07280 and HSERO_RS07285 are separated by 30 nucleotides HSERO_RS07285 and HSERO_RS07290 are separated by 343 nucleotides
HSERO_RS07275: HSERO_RS07275 - D-amino acid dehydrogenase, at 1,664,510 to 1,665,820
_RS07275
HSERO_RS07280: HSERO_RS07280 - hypothetical protein, at 1,666,013 to 1,666,453
_RS07280
HSERO_RS07285: HSERO_RS07285 - DNA polymerase III subunit chi, at 1,666,484 to 1,666,903
_RS07285
HSERO_RS07290: HSERO_RS07290 - oligopeptidase A, at 1,667,247 to 1,669,322
_RS07290
Position (kb)
1666
1667 Strain fitness (log2 ratio)
-2
-1
0
1 at 1665.199 kb on - strand, within HSERO_RS07275 at 1665.199 kb on - strand, within HSERO_RS07275 at 1665.396 kb on + strand, within HSERO_RS07275 at 1665.404 kb on + strand, within HSERO_RS07275 at 1665.593 kb on + strand, within HSERO_RS07275 at 1666.138 kb on - strand, within HSERO_RS07280 at 1666.146 kb on + strand, within HSERO_RS07280 at 1666.380 kb on - strand, within HSERO_RS07280 at 1666.472 kb on + strand at 1666.515 kb on - strand at 1666.911 kb on + strand at 1666.916 kb on - strand at 1666.987 kb on + strand at 1666.987 kb on + strand at 1666.995 kb on - strand at 1666.997 kb on + strand at 1667.005 kb on - strand at 1667.167 kb on + strand at 1667.176 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Taped volatile agar plate with no fungus remove 1,665,199 - HSERO_RS07275 0.53 -0.6 1,665,199 - HSERO_RS07275 0.53 -0.5 1,665,396 + HSERO_RS07275 0.68 -0.9 1,665,404 + HSERO_RS07275 0.68 +0.2 1,665,593 + HSERO_RS07275 0.83 -1.1 1,666,138 - HSERO_RS07280 0.28 -1.0 1,666,146 + HSERO_RS07280 0.30 -0.6 1,666,380 - HSERO_RS07280 0.83 -0.9 1,666,472 + -2.2 1,666,515 - +0.0 1,666,911 + -2.5 1,666,916 - +0.8 1,666,987 + -1.0 1,666,987 + +0.1 1,666,995 - +0.6 1,666,997 + -1.2 1,667,005 - +0.0 1,667,167 + -0.5 1,667,176 + +1.1
Or see this region's nucleotide sequence