Experiment: Taped volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS04065 and HSERO_RS04070 are separated by 90 nucleotides HSERO_RS04070 and HSERO_RS04075 overlap by 4 nucleotides HSERO_RS04075 and HSERO_RS04080 overlap by 20 nucleotides HSERO_RS04080 and HSERO_RS04085 are separated by 228 nucleotides
HSERO_RS04065: HSERO_RS04065 - pilus biosynthesis protein PilS, at 890,220 to 890,771
_RS04065
HSERO_RS04070: HSERO_RS04070 - hypothetical protein, at 890,862 to 891,359
_RS04070
HSERO_RS04075: HSERO_RS04075 - type IV prepilin PilV protein, at 891,356 to 892,759
_RS04075
HSERO_RS04080: HSERO_RS04080 - invasion protein, at 892,740 to 893,237
_RS04080
HSERO_RS04085: HSERO_RS04085 - twitching motility protein PilT, at 893,466 to 894,563
_RS04085
Position (kb) 891
892
893 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 890.378 kb on + strand, within HSERO_RS04065 at 890.431 kb on - strand, within HSERO_RS04065 at 890.456 kb on + strand, within HSERO_RS04065 at 890.610 kb on - strand, within HSERO_RS04065 at 890.612 kb on + strand, within HSERO_RS04065 at 890.761 kb on - strand at 890.848 kb on + strand at 890.853 kb on - strand at 891.029 kb on + strand, within HSERO_RS04070 at 891.067 kb on + strand, within HSERO_RS04070 at 891.075 kb on - strand, within HSERO_RS04070 at 891.124 kb on - strand, within HSERO_RS04070 at 891.163 kb on + strand, within HSERO_RS04070 at 891.343 kb on - strand at 891.384 kb on + strand at 891.553 kb on + strand, within HSERO_RS04075 at 891.610 kb on + strand, within HSERO_RS04075 at 891.646 kb on + strand, within HSERO_RS04075 at 892.161 kb on + strand, within HSERO_RS04075 at 892.169 kb on - strand, within HSERO_RS04075 at 892.169 kb on - strand, within HSERO_RS04075 at 892.245 kb on + strand, within HSERO_RS04075 at 892.309 kb on - strand, within HSERO_RS04075 at 892.438 kb on + strand, within HSERO_RS04075 at 892.438 kb on + strand, within HSERO_RS04075 at 892.446 kb on - strand, within HSERO_RS04075 at 892.747 kb on + strand at 892.770 kb on - strand at 892.823 kb on + strand, within HSERO_RS04080 at 892.857 kb on + strand, within HSERO_RS04080 at 892.867 kb on + strand, within HSERO_RS04080 at 892.910 kb on + strand, within HSERO_RS04080 at 892.918 kb on - strand, within HSERO_RS04080 at 892.954 kb on - strand, within HSERO_RS04080 at 892.954 kb on - strand, within HSERO_RS04080 at 892.954 kb on - strand, within HSERO_RS04080 at 893.121 kb on + strand, within HSERO_RS04080 at 893.161 kb on + strand, within HSERO_RS04080 at 893.161 kb on + strand, within HSERO_RS04080 at 893.169 kb on - strand, within HSERO_RS04080 at 893.169 kb on - strand, within HSERO_RS04080 at 893.242 kb on - strand at 893.392 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Taped volatile agar plate with no fungus remove 890,378 + HSERO_RS04065 0.29 -0.1 890,431 - HSERO_RS04065 0.38 +2.2 890,456 + HSERO_RS04065 0.43 +0.2 890,610 - HSERO_RS04065 0.71 -0.2 890,612 + HSERO_RS04065 0.71 +3.1 890,761 - +0.5 890,848 + +1.1 890,853 - -0.3 891,029 + HSERO_RS04070 0.34 +0.5 891,067 + HSERO_RS04070 0.41 -1.0 891,075 - HSERO_RS04070 0.43 +1.0 891,124 - HSERO_RS04070 0.53 +0.4 891,163 + HSERO_RS04070 0.60 -1.3 891,343 - -0.5 891,384 + -0.3 891,553 + HSERO_RS04075 0.14 +1.0 891,610 + HSERO_RS04075 0.18 -1.5 891,646 + HSERO_RS04075 0.21 +0.1 892,161 + HSERO_RS04075 0.57 +0.1 892,169 - HSERO_RS04075 0.58 -1.5 892,169 - HSERO_RS04075 0.58 +0.4 892,245 + HSERO_RS04075 0.63 +1.0 892,309 - HSERO_RS04075 0.68 +2.2 892,438 + HSERO_RS04075 0.77 +1.0 892,438 + HSERO_RS04075 0.77 +1.1 892,446 - HSERO_RS04075 0.78 +0.2 892,747 + -2.1 892,770 - +1.6 892,823 + HSERO_RS04080 0.17 -0.6 892,857 + HSERO_RS04080 0.23 +0.2 892,867 + HSERO_RS04080 0.26 +0.4 892,910 + HSERO_RS04080 0.34 -0.5 892,918 - HSERO_RS04080 0.36 +0.2 892,954 - HSERO_RS04080 0.43 +0.0 892,954 - HSERO_RS04080 0.43 -1.0 892,954 - HSERO_RS04080 0.43 +0.1 893,121 + HSERO_RS04080 0.77 -0.6 893,161 + HSERO_RS04080 0.85 -0.3 893,161 + HSERO_RS04080 0.85 +0.5 893,169 - HSERO_RS04080 0.86 +0.9 893,169 - HSERO_RS04080 0.86 +1.0 893,242 - -0.8 893,392 + -0.3
Or see this region's nucleotide sequence