Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS19805 and HSERO_RS19810 are separated by 217 nucleotides HSERO_RS19810 and HSERO_RS19815 are separated by 171 nucleotides HSERO_RS19815 and HSERO_RS19820 overlap by 73 nucleotides
HSERO_RS19805: HSERO_RS19805 - glycosyl transferase, at 4,544,423 to 4,546,177
_RS19805
HSERO_RS19810: HSERO_RS19810 - excinuclease ABC subunit A, at 4,546,395 to 4,549,253
_RS19810
HSERO_RS19815: HSERO_RS19815 - phytanoyl-CoA dioxygenase, at 4,549,425 to 4,550,192
_RS19815
HSERO_RS19820: HSERO_RS19820 - methyl-accepting chemotaxis protein, at 4,550,120 to 4,551,790
_RS19820
Position (kb)
4546
4547
4548
4549
4550 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 4546.016 kb on + strand at 4546.206 kb on - strand at 4546.301 kb on + strand at 4546.309 kb on - strand at 4546.333 kb on + strand at 4546.434 kb on - strand at 4546.434 kb on - strand at 4546.756 kb on - strand, within HSERO_RS19810 at 4546.994 kb on - strand, within HSERO_RS19810 at 4547.008 kb on + strand, within HSERO_RS19810 at 4547.027 kb on - strand, within HSERO_RS19810 at 4547.036 kb on + strand, within HSERO_RS19810 at 4547.072 kb on - strand, within HSERO_RS19810 at 4547.390 kb on - strand, within HSERO_RS19810 at 4547.400 kb on - strand, within HSERO_RS19810 at 4547.595 kb on - strand, within HSERO_RS19810 at 4547.665 kb on + strand, within HSERO_RS19810 at 4547.760 kb on - strand, within HSERO_RS19810 at 4547.770 kb on - strand at 4547.851 kb on - strand, within HSERO_RS19810 at 4547.928 kb on + strand, within HSERO_RS19810 at 4547.973 kb on - strand, within HSERO_RS19810 at 4548.208 kb on + strand, within HSERO_RS19810 at 4548.292 kb on - strand, within HSERO_RS19810 at 4548.306 kb on - strand, within HSERO_RS19810 at 4548.326 kb on - strand, within HSERO_RS19810 at 4548.326 kb on - strand, within HSERO_RS19810 at 4548.363 kb on - strand at 4548.370 kb on + strand, within HSERO_RS19810 at 4548.370 kb on + strand, within HSERO_RS19810 at 4548.386 kb on + strand, within HSERO_RS19810 at 4548.482 kb on - strand, within HSERO_RS19810 at 4548.483 kb on - strand, within HSERO_RS19810 at 4548.723 kb on + strand, within HSERO_RS19810 at 4548.724 kb on - strand, within HSERO_RS19810 at 4548.747 kb on + strand, within HSERO_RS19810 at 4548.758 kb on + strand, within HSERO_RS19810 at 4548.880 kb on + strand, within HSERO_RS19810 at 4548.950 kb on - strand, within HSERO_RS19810 at 4548.955 kb on - strand, within HSERO_RS19810 at 4549.023 kb on - strand at 4549.036 kb on - strand at 4549.058 kb on + strand at 4549.292 kb on + strand at 4549.339 kb on + strand at 4549.484 kb on + strand at 4549.616 kb on + strand, within HSERO_RS19815 at 4549.811 kb on - strand, within HSERO_RS19815 at 4549.932 kb on - strand, within HSERO_RS19815 at 4550.035 kb on + strand, within HSERO_RS19815 at 4550.053 kb on - strand, within HSERO_RS19815 at 4550.093 kb on + strand, within HSERO_RS19815 at 4550.101 kb on - strand, within HSERO_RS19815 at 4550.181 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 4,546,016 + -1.5 4,546,206 - -0.9 4,546,301 + -1.8 4,546,309 - -0.5 4,546,333 + -0.5 4,546,434 - -1.8 4,546,434 - -0.1 4,546,756 - HSERO_RS19810 0.13 -0.4 4,546,994 - HSERO_RS19810 0.21 -0.2 4,547,008 + HSERO_RS19810 0.21 -0.5 4,547,027 - HSERO_RS19810 0.22 -0.6 4,547,036 + HSERO_RS19810 0.22 -0.6 4,547,072 - HSERO_RS19810 0.24 -0.1 4,547,390 - HSERO_RS19810 0.35 -0.1 4,547,400 - HSERO_RS19810 0.35 -2.1 4,547,595 - HSERO_RS19810 0.42 -0.7 4,547,665 + HSERO_RS19810 0.44 +0.1 4,547,760 - HSERO_RS19810 0.48 -0.8 4,547,770 - +4.9 4,547,851 - HSERO_RS19810 0.51 +0.5 4,547,928 + HSERO_RS19810 0.54 -0.4 4,547,973 - HSERO_RS19810 0.55 +2.9 4,548,208 + HSERO_RS19810 0.63 -0.8 4,548,292 - HSERO_RS19810 0.66 -0.1 4,548,306 - HSERO_RS19810 0.67 -0.7 4,548,326 - HSERO_RS19810 0.68 +0.4 4,548,326 - HSERO_RS19810 0.68 +0.9 4,548,363 - -0.3 4,548,370 + HSERO_RS19810 0.69 -2.0 4,548,370 + HSERO_RS19810 0.69 +0.1 4,548,386 + HSERO_RS19810 0.70 +0.8 4,548,482 - HSERO_RS19810 0.73 -1.9 4,548,483 - HSERO_RS19810 0.73 -1.4 4,548,723 + HSERO_RS19810 0.81 -3.3 4,548,724 - HSERO_RS19810 0.81 -0.4 4,548,747 + HSERO_RS19810 0.82 -1.3 4,548,758 + HSERO_RS19810 0.83 -1.0 4,548,880 + HSERO_RS19810 0.87 -0.0 4,548,950 - HSERO_RS19810 0.89 -0.4 4,548,955 - HSERO_RS19810 0.90 -1.3 4,549,023 - -0.7 4,549,036 - -0.4 4,549,058 + -2.0 4,549,292 + +0.7 4,549,339 + +0.0 4,549,484 + -0.8 4,549,616 + HSERO_RS19815 0.25 +0.8 4,549,811 - HSERO_RS19815 0.50 -0.5 4,549,932 - HSERO_RS19815 0.66 -0.3 4,550,035 + HSERO_RS19815 0.79 -1.0 4,550,053 - HSERO_RS19815 0.82 +0.1 4,550,093 + HSERO_RS19815 0.87 +1.4 4,550,101 - HSERO_RS19815 0.88 +0.2 4,550,181 + -2.7
Or see this region's nucleotide sequence