Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS17900 and HSERO_RS17905 are separated by 75 nucleotides HSERO_RS17905 and HSERO_RS17910 are separated by 78 nucleotides HSERO_RS17910 and HSERO_RS17915 are separated by 38 nucleotides
HSERO_RS17900: HSERO_RS17900 - 3,4-dihydroxy-2-butanone 4-phosphate synthase, at 4,114,615 to 4,115,736
_RS17900
HSERO_RS17905: HSERO_RS17905 - tRNA-dihydrouridine synthase A, at 4,115,812 to 4,116,858
_RS17905
HSERO_RS17910: HSERO_RS17910 - hypothetical protein, at 4,116,937 to 4,117,302
_RS17910
HSERO_RS17915: HSERO_RS17915 - transposase, at 4,117,341 to 4,117,715
_RS17915
Position (kb) 4115
4116
4117 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4114.900 kb on + strand, within HSERO_RS17900 at 4114.906 kb on - strand, within HSERO_RS17900 at 4115.328 kb on - strand, within HSERO_RS17900 at 4115.822 kb on + strand at 4115.868 kb on + strand at 4116.039 kb on - strand, within HSERO_RS17905 at 4116.087 kb on - strand, within HSERO_RS17905 at 4116.119 kb on + strand, within HSERO_RS17905 at 4116.127 kb on - strand, within HSERO_RS17905 at 4116.264 kb on - strand, within HSERO_RS17905 at 4116.397 kb on + strand, within HSERO_RS17905 at 4116.398 kb on + strand, within HSERO_RS17905 at 4116.450 kb on + strand, within HSERO_RS17905 at 4116.472 kb on - strand, within HSERO_RS17905 at 4116.553 kb on + strand, within HSERO_RS17905 at 4116.599 kb on - strand, within HSERO_RS17905 at 4116.660 kb on - strand, within HSERO_RS17905 at 4116.686 kb on + strand, within HSERO_RS17905 at 4116.873 kb on + strand at 4116.963 kb on + strand at 4116.963 kb on + strand at 4116.971 kb on - strand at 4117.038 kb on + strand, within HSERO_RS17910 at 4117.046 kb on - strand, within HSERO_RS17910 at 4117.046 kb on - strand, within HSERO_RS17910 at 4117.113 kb on + strand, within HSERO_RS17910 at 4117.155 kb on + strand, within HSERO_RS17910 at 4117.155 kb on + strand, within HSERO_RS17910 at 4117.163 kb on - strand, within HSERO_RS17910 at 4117.234 kb on - strand, within HSERO_RS17910 at 4117.296 kb on + strand at 4117.304 kb on + strand at 4117.312 kb on - strand at 4117.314 kb on + strand at 4117.314 kb on + strand at 4117.314 kb on + strand at 4117.339 kb on - strand at 4117.339 kb on - strand at 4117.341 kb on + strand at 4117.361 kb on - strand at 4117.376 kb on - strand at 4117.379 kb on + strand, within HSERO_RS17915 at 4117.383 kb on + strand, within HSERO_RS17915 at 4117.428 kb on - strand, within HSERO_RS17915 at 4117.441 kb on + strand, within HSERO_RS17915 at 4117.441 kb on - strand, within HSERO_RS17915 at 4117.536 kb on + strand, within HSERO_RS17915 at 4117.541 kb on + strand, within HSERO_RS17915 at 4117.554 kb on - strand, within HSERO_RS17915 at 4117.685 kb on + strand at 4117.685 kb on + strand at 4117.799 kb on + strand at 4117.799 kb on + strand at 4117.822 kb on + strand at 4117.822 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 4,114,900 + HSERO_RS17900 0.25 -0.5 4,114,906 - HSERO_RS17900 0.26 -0.5 4,115,328 - HSERO_RS17900 0.64 -2.2 4,115,822 + +1.1 4,115,868 + +1.8 4,116,039 - HSERO_RS17905 0.22 -1.1 4,116,087 - HSERO_RS17905 0.26 +0.2 4,116,119 + HSERO_RS17905 0.29 -0.5 4,116,127 - HSERO_RS17905 0.30 -1.0 4,116,264 - HSERO_RS17905 0.43 -2.8 4,116,397 + HSERO_RS17905 0.56 -2.2 4,116,398 + HSERO_RS17905 0.56 -0.6 4,116,450 + HSERO_RS17905 0.61 -1.5 4,116,472 - HSERO_RS17905 0.63 +0.4 4,116,553 + HSERO_RS17905 0.71 +1.0 4,116,599 - HSERO_RS17905 0.75 -0.3 4,116,660 - HSERO_RS17905 0.81 -0.8 4,116,686 + HSERO_RS17905 0.83 -0.5 4,116,873 + -0.4 4,116,963 + +0.3 4,116,963 + -1.0 4,116,971 - -0.6 4,117,038 + HSERO_RS17910 0.28 +0.5 4,117,046 - HSERO_RS17910 0.30 +0.1 4,117,046 - HSERO_RS17910 0.30 +0.2 4,117,113 + HSERO_RS17910 0.48 -0.2 4,117,155 + HSERO_RS17910 0.60 +0.5 4,117,155 + HSERO_RS17910 0.60 +0.0 4,117,163 - HSERO_RS17910 0.62 +0.4 4,117,234 - HSERO_RS17910 0.81 +0.1 4,117,296 + +0.3 4,117,304 + -0.4 4,117,312 - +0.2 4,117,314 + -0.3 4,117,314 + -1.0 4,117,314 + +0.4 4,117,339 - +0.4 4,117,339 - -1.4 4,117,341 + +0.3 4,117,361 - +0.4 4,117,376 - -2.6 4,117,379 + HSERO_RS17915 0.10 +0.7 4,117,383 + HSERO_RS17915 0.11 -0.0 4,117,428 - HSERO_RS17915 0.23 -1.4 4,117,441 + HSERO_RS17915 0.27 -0.7 4,117,441 - HSERO_RS17915 0.27 +1.1 4,117,536 + HSERO_RS17915 0.52 +0.8 4,117,541 + HSERO_RS17915 0.53 -1.5 4,117,554 - HSERO_RS17915 0.57 +0.2 4,117,685 + +0.1 4,117,685 + -0.7 4,117,799 + -0.4 4,117,799 + -0.2 4,117,822 + -0.2 4,117,822 - +0.4
Or see this region's nucleotide sequence