Experiment: BHIS with Zinc Pyrithione 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1452 and BT1453 are separated by 240 nucleotides BT1453 and BT1454 are separated by 290 nucleotides BT1454 and BT1455 are separated by 4 nucleotides
BT1452: BT1452 - conserved hypothetical protein (NCBI ptt file), at 1,788,165 to 1,789,619
BT1452
BT1453: BT1453 - L-lactate permease (NCBI ptt file), at 1,789,860 to 1,791,359
BT1453
BT1454: BT1454 - putative peptide chain release factor (NCBI ptt file), at 1,791,650 to 1,792,270
BT1454
BT1455: BT1455 - protein rtcB (NCBI ptt file), at 1,792,275 to 1,793,672
BT1455
Position (kb)
1789
1790
1791
1792 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1789.167 kb on + strand, within BT1452 at 1789.291 kb on + strand, within BT1452 at 1789.298 kb on + strand, within BT1452 at 1789.299 kb on - strand, within BT1452 at 1789.299 kb on - strand, within BT1452 at 1789.303 kb on - strand, within BT1452 at 1789.328 kb on - strand, within BT1452 at 1789.348 kb on + strand, within BT1452 at 1789.352 kb on + strand, within BT1452 at 1789.352 kb on + strand, within BT1452 at 1789.352 kb on + strand, within BT1452 at 1789.353 kb on - strand, within BT1452 at 1789.353 kb on - strand, within BT1452 at 1789.353 kb on - strand, within BT1452 at 1789.357 kb on - strand, within BT1452 at 1789.372 kb on + strand, within BT1452 at 1789.434 kb on + strand, within BT1452 at 1789.489 kb on - strand at 1789.490 kb on + strand at 1789.509 kb on - strand at 1789.520 kb on + strand at 1789.521 kb on - strand at 1789.549 kb on + strand at 1789.602 kb on - strand at 1789.643 kb on + strand at 1789.696 kb on - strand at 1789.740 kb on - strand at 1789.788 kb on - strand at 1789.820 kb on + strand at 1789.878 kb on - strand at 1789.904 kb on - strand at 1789.904 kb on - strand at 1789.947 kb on - strand at 1789.961 kb on - strand at 1789.961 kb on - strand at 1790.023 kb on + strand, within BT1453 at 1790.024 kb on - strand, within BT1453 at 1790.026 kb on - strand, within BT1453 at 1790.026 kb on - strand, within BT1453 at 1790.056 kb on + strand, within BT1453 at 1790.056 kb on + strand, within BT1453 at 1790.057 kb on - strand, within BT1453 at 1790.120 kb on + strand, within BT1453 at 1790.159 kb on + strand, within BT1453 at 1790.235 kb on - strand, within BT1453 at 1790.275 kb on + strand at 1790.390 kb on + strand, within BT1453 at 1790.549 kb on + strand, within BT1453 at 1790.550 kb on - strand, within BT1453 at 1790.591 kb on + strand, within BT1453 at 1790.592 kb on - strand, within BT1453 at 1790.673 kb on - strand, within BT1453 at 1790.674 kb on + strand, within BT1453 at 1790.753 kb on + strand, within BT1453 at 1790.814 kb on + strand, within BT1453 at 1790.817 kb on - strand, within BT1453 at 1790.909 kb on + strand, within BT1453 at 1790.924 kb on + strand, within BT1453 at 1790.939 kb on - strand, within BT1453 at 1791.032 kb on + strand, within BT1453 at 1791.093 kb on - strand, within BT1453 at 1791.108 kb on - strand, within BT1453 at 1791.200 kb on - strand, within BT1453 at 1791.318 kb on + strand at 1791.318 kb on + strand at 1791.330 kb on - strand at 1791.373 kb on + strand at 1791.377 kb on - strand at 1791.404 kb on + strand at 1791.404 kb on - strand at 1791.405 kb on - strand at 1791.406 kb on + strand at 1791.415 kb on + strand at 1791.415 kb on + strand at 1791.424 kb on + strand at 1791.424 kb on + strand at 1791.425 kb on - strand at 1791.429 kb on - strand at 1791.429 kb on - strand at 1791.450 kb on + strand at 1791.528 kb on + strand at 1791.540 kb on - strand at 1791.551 kb on + strand at 1791.552 kb on - strand at 1791.604 kb on + strand at 1791.629 kb on + strand at 1791.703 kb on + strand at 1791.710 kb on - strand at 1792.245 kb on - strand at 1792.307 kb on + strand at 1792.345 kb on + strand at 1792.345 kb on + strand at 1792.346 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Zinc Pyrithione 0.0125 mM remove 1,789,167 + BT1452 0.69 -0.8 1,789,291 + BT1452 0.77 -0.9 1,789,298 + BT1452 0.78 -0.0 1,789,299 - BT1452 0.78 -1.4 1,789,299 - BT1452 0.78 -1.0 1,789,303 - BT1452 0.78 -2.2 1,789,328 - BT1452 0.80 -1.4 1,789,348 + BT1452 0.81 -2.7 1,789,352 + BT1452 0.82 -0.7 1,789,352 + BT1452 0.82 -1.8 1,789,352 + BT1452 0.82 -2.2 1,789,353 - BT1452 0.82 -0.7 1,789,353 - BT1452 0.82 +0.2 1,789,353 - BT1452 0.82 +0.2 1,789,357 - BT1452 0.82 -0.8 1,789,372 + BT1452 0.83 -2.0 1,789,434 + BT1452 0.87 -0.4 1,789,489 - -2.0 1,789,490 + -3.5 1,789,509 - +0.2 1,789,520 + -0.2 1,789,521 - -2.7 1,789,549 + -0.8 1,789,602 - -2.4 1,789,643 + +0.2 1,789,696 - -0.4 1,789,740 - +1.2 1,789,788 - -1.4 1,789,820 + +1.7 1,789,878 - -1.4 1,789,904 - +0.5 1,789,904 - -1.8 1,789,947 - +0.2 1,789,961 - -1.0 1,789,961 - -0.8 1,790,023 + BT1453 0.11 +0.2 1,790,024 - BT1453 0.11 +0.2 1,790,026 - BT1453 0.11 -2.4 1,790,026 - BT1453 0.11 -1.4 1,790,056 + BT1453 0.13 +0.2 1,790,056 + BT1453 0.13 +1.2 1,790,057 - BT1453 0.13 -2.3 1,790,120 + BT1453 0.17 -0.8 1,790,159 + BT1453 0.20 +0.2 1,790,235 - BT1453 0.25 -3.8 1,790,275 + +0.2 1,790,390 + BT1453 0.35 -2.8 1,790,549 + BT1453 0.46 -0.8 1,790,550 - BT1453 0.46 -1.7 1,790,591 + BT1453 0.49 -0.8 1,790,592 - BT1453 0.49 -3.1 1,790,673 - BT1453 0.54 -2.2 1,790,674 + BT1453 0.54 -2.7 1,790,753 + BT1453 0.60 -2.8 1,790,814 + BT1453 0.64 -2.7 1,790,817 - BT1453 0.64 -1.4 1,790,909 + BT1453 0.70 -1.7 1,790,924 + BT1453 0.71 -2.2 1,790,939 - BT1453 0.72 -2.2 1,791,032 + BT1453 0.78 +0.2 1,791,093 - BT1453 0.82 +0.6 1,791,108 - BT1453 0.83 -1.6 1,791,200 - BT1453 0.89 -2.4 1,791,318 + -2.2 1,791,318 + -0.8 1,791,330 - -1.8 1,791,373 + -1.1 1,791,377 - -1.8 1,791,404 + -1.8 1,791,404 - +1.2 1,791,405 - +0.2 1,791,406 + -1.8 1,791,415 + +0.2 1,791,415 + -1.5 1,791,424 + -1.4 1,791,424 + -1.4 1,791,425 - +1.2 1,791,429 - +0.2 1,791,429 - -1.4 1,791,450 + +0.0 1,791,528 + +0.2 1,791,540 - -0.8 1,791,551 + -0.8 1,791,552 - -1.4 1,791,604 + -1.4 1,791,629 + -1.2 1,791,703 + -0.8 1,791,710 - -1.8 1,792,245 - -1.4 1,792,307 + +0.2 1,792,345 + +1.2 1,792,345 + -3.0 1,792,346 - -0.6
Or see this region's nucleotide sequence