Experiment: KB with Paclitaxel 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2028 and Psyr_2029 are separated by 69 nucleotides Psyr_2029 and Psyr_2030 are separated by 45 nucleotides Psyr_2030 and Psyr_2031 are separated by 115 nucleotides
Psyr_2028: Psyr_2028 - conserved hypothetical protein, at 2,352,422 to 2,353,252
_2028
Psyr_2029: Psyr_2029 - Protein of unknown function UPF0118, at 2,353,322 to 2,354,368
_2029
Psyr_2030: Psyr_2030 - Calcium-binding EF-hand, at 2,354,414 to 2,355,169
_2030
Psyr_2031: Psyr_2031 - ATP-binding region, ATPase-like:Histidine kinase, HAMP region:Histidine kinase A, N-terminal, at 2,355,285 to 2,356,319
_2031
Position (kb)
2353
2354
2355 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2352.394 kb on + strand at 2352.394 kb on + strand at 2352.395 kb on - strand at 2352.507 kb on + strand, within Psyr_2028 at 2352.507 kb on + strand, within Psyr_2028 at 2352.508 kb on - strand, within Psyr_2028 at 2352.508 kb on - strand, within Psyr_2028 at 2352.508 kb on - strand, within Psyr_2028 at 2352.684 kb on - strand, within Psyr_2028 at 2352.690 kb on + strand, within Psyr_2028 at 2352.690 kb on + strand, within Psyr_2028 at 2352.691 kb on - strand, within Psyr_2028 at 2352.691 kb on - strand, within Psyr_2028 at 2352.691 kb on - strand, within Psyr_2028 at 2352.839 kb on + strand, within Psyr_2028 at 2352.840 kb on - strand, within Psyr_2028 at 2352.914 kb on + strand, within Psyr_2028 at 2352.914 kb on + strand, within Psyr_2028 at 2352.915 kb on - strand, within Psyr_2028 at 2352.944 kb on + strand, within Psyr_2028 at 2352.945 kb on - strand, within Psyr_2028 at 2353.084 kb on + strand, within Psyr_2028 at 2353.250 kb on + strand at 2353.284 kb on + strand at 2353.285 kb on - strand at 2353.835 kb on + strand, within Psyr_2029 at 2353.836 kb on - strand, within Psyr_2029 at 2353.907 kb on + strand, within Psyr_2029 at 2353.907 kb on + strand, within Psyr_2029 at 2353.908 kb on - strand, within Psyr_2029 at 2353.945 kb on - strand, within Psyr_2029 at 2354.198 kb on + strand, within Psyr_2029 at 2354.198 kb on + strand, within Psyr_2029 at 2354.199 kb on - strand, within Psyr_2029 at 2354.199 kb on - strand, within Psyr_2029 at 2354.199 kb on - strand, within Psyr_2029 at 2354.223 kb on + strand, within Psyr_2029 at 2354.319 kb on - strand at 2354.467 kb on - strand at 2354.467 kb on - strand at 2354.587 kb on + strand, within Psyr_2030 at 2354.615 kb on - strand, within Psyr_2030 at 2354.615 kb on - strand, within Psyr_2030 at 2354.672 kb on - strand, within Psyr_2030 at 2355.135 kb on + strand at 2355.136 kb on - strand at 2355.155 kb on + strand at 2355.253 kb on + strand at 2355.253 kb on + strand at 2355.254 kb on - strand at 2355.254 kb on - strand at 2355.254 kb on - strand at 2355.254 kb on - strand at 2355.254 kb on - strand at 2355.262 kb on + strand at 2355.262 kb on + strand at 2355.262 kb on + strand at 2355.262 kb on + strand at 2355.263 kb on - strand at 2355.263 kb on - strand at 2355.281 kb on + strand at 2355.281 kb on + strand at 2355.281 kb on + strand at 2355.281 kb on + strand at 2355.281 kb on + strand at 2355.282 kb on - strand at 2355.282 kb on - strand at 2355.282 kb on - strand at 2355.282 kb on - strand at 2355.282 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Paclitaxel 10 mM remove 2,352,394 + +1.2 2,352,394 + -0.1 2,352,395 - -0.3 2,352,507 + Psyr_2028 0.10 -0.2 2,352,507 + Psyr_2028 0.10 -1.3 2,352,508 - Psyr_2028 0.10 -0.1 2,352,508 - Psyr_2028 0.10 +3.0 2,352,508 - Psyr_2028 0.10 -0.6 2,352,684 - Psyr_2028 0.32 -2.6 2,352,690 + Psyr_2028 0.32 -0.7 2,352,690 + Psyr_2028 0.32 +0.2 2,352,691 - Psyr_2028 0.32 -1.0 2,352,691 - Psyr_2028 0.32 +2.0 2,352,691 - Psyr_2028 0.32 -1.3 2,352,839 + Psyr_2028 0.50 +0.7 2,352,840 - Psyr_2028 0.50 +0.7 2,352,914 + Psyr_2028 0.59 -1.0 2,352,914 + Psyr_2028 0.59 -1.7 2,352,915 - Psyr_2028 0.59 +0.3 2,352,944 + Psyr_2028 0.63 -2.0 2,352,945 - Psyr_2028 0.63 +0.8 2,353,084 + Psyr_2028 0.80 +1.0 2,353,250 + -1.4 2,353,284 + -0.2 2,353,285 - -1.6 2,353,835 + Psyr_2029 0.49 +0.5 2,353,836 - Psyr_2029 0.49 -1.3 2,353,907 + Psyr_2029 0.56 -1.0 2,353,907 + Psyr_2029 0.56 +1.0 2,353,908 - Psyr_2029 0.56 -2.2 2,353,945 - Psyr_2029 0.60 -1.6 2,354,198 + Psyr_2029 0.84 -1.3 2,354,198 + Psyr_2029 0.84 -1.4 2,354,199 - Psyr_2029 0.84 -1.4 2,354,199 - Psyr_2029 0.84 -0.5 2,354,199 - Psyr_2029 0.84 +0.3 2,354,223 + Psyr_2029 0.86 -1.0 2,354,319 - -1.0 2,354,467 - +0.3 2,354,467 - -2.8 2,354,587 + Psyr_2030 0.23 +0.6 2,354,615 - Psyr_2030 0.27 +0.3 2,354,615 - Psyr_2030 0.27 -0.5 2,354,672 - Psyr_2030 0.34 -2.0 2,355,135 + +0.2 2,355,136 - +1.0 2,355,155 + -1.5 2,355,253 + -0.4 2,355,253 + -0.4 2,355,254 - -1.2 2,355,254 - +0.8 2,355,254 - -0.5 2,355,254 - -1.2 2,355,254 - -0.5 2,355,262 + +0.0 2,355,262 + -0.4 2,355,262 + -2.2 2,355,262 + -1.8 2,355,263 - -0.7 2,355,263 - -1.3 2,355,281 + +1.5 2,355,281 + +1.9 2,355,281 + +1.0 2,355,281 + +1.0 2,355,281 + +1.0 2,355,282 - +0.6 2,355,282 - -0.4 2,355,282 - +1.7 2,355,282 - +0.9 2,355,282 - +0.6
Or see this region's nucleotide sequence