Experiment: KB with Paclitaxel 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0149 and Psyr_0150 are separated by 114 nucleotides Psyr_0150 and Psyr_0151 overlap by 4 nucleotides
Psyr_0149: Psyr_0149 - succinate semialdehyde dehydrogenase, at 161,189 to 162,646
_0149
Psyr_0150: Psyr_0150 - Acetylornithine deacetylase (ArgE), at 162,761 to 163,918
_0150
Psyr_0151: Psyr_0151 - FAD dependent oxidoreductase, at 163,915 to 165,084
_0151
Position (kb) 162
163
164 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 162.037 kb on + strand, within Psyr_0149 at 162.258 kb on + strand, within Psyr_0149 at 162.259 kb on - strand, within Psyr_0149 at 162.597 kb on + strand at 162.597 kb on + strand at 162.598 kb on - strand at 162.598 kb on - strand at 162.608 kb on + strand at 162.680 kb on - strand at 162.763 kb on - strand at 162.763 kb on - strand at 162.927 kb on + strand, within Psyr_0150 at 162.928 kb on - strand, within Psyr_0150 at 162.928 kb on - strand, within Psyr_0150 at 163.076 kb on + strand, within Psyr_0150 at 163.076 kb on + strand, within Psyr_0150 at 163.077 kb on - strand, within Psyr_0150 at 163.217 kb on + strand, within Psyr_0150 at 163.218 kb on - strand, within Psyr_0150 at 163.218 kb on - strand, within Psyr_0150 at 163.218 kb on - strand, within Psyr_0150 at 163.218 kb on - strand, within Psyr_0150 at 163.227 kb on + strand, within Psyr_0150 at 163.227 kb on + strand, within Psyr_0150 at 163.227 kb on + strand, within Psyr_0150 at 163.457 kb on + strand, within Psyr_0150 at 163.458 kb on - strand, within Psyr_0150 at 163.458 kb on - strand, within Psyr_0150 at 163.629 kb on + strand, within Psyr_0150 at 163.629 kb on + strand, within Psyr_0150 at 163.630 kb on - strand, within Psyr_0150 at 163.630 kb on - strand, within Psyr_0150 at 163.630 kb on - strand, within Psyr_0150 at 163.630 kb on - strand, within Psyr_0150 at 163.833 kb on + strand at 163.834 kb on - strand at 163.834 kb on - strand at 163.926 kb on + strand at 163.927 kb on - strand at 164.087 kb on + strand, within Psyr_0151 at 164.087 kb on + strand, within Psyr_0151 at 164.087 kb on + strand, within Psyr_0151 at 164.087 kb on + strand, within Psyr_0151 at 164.088 kb on - strand, within Psyr_0151 at 164.088 kb on - strand, within Psyr_0151 at 164.088 kb on - strand, within Psyr_0151 at 164.088 kb on - strand, within Psyr_0151 at 164.088 kb on - strand, within Psyr_0151 at 164.088 kb on - strand, within Psyr_0151 at 164.088 kb on - strand, within Psyr_0151 at 164.250 kb on - strand, within Psyr_0151 at 164.330 kb on + strand, within Psyr_0151 at 164.330 kb on + strand, within Psyr_0151 at 164.330 kb on + strand, within Psyr_0151 at 164.330 kb on + strand, within Psyr_0151 at 164.331 kb on - strand, within Psyr_0151 at 164.666 kb on + strand, within Psyr_0151 at 164.667 kb on - strand, within Psyr_0151 at 164.729 kb on + strand, within Psyr_0151 at 164.729 kb on + strand, within Psyr_0151 at 164.729 kb on + strand, within Psyr_0151 at 164.729 kb on + strand, within Psyr_0151 at 164.729 kb on + strand, within Psyr_0151 at 164.729 kb on + strand, within Psyr_0151 at 164.730 kb on - strand, within Psyr_0151 at 164.771 kb on + strand, within Psyr_0151 at 164.771 kb on + strand, within Psyr_0151 at 164.772 kb on - strand, within Psyr_0151 at 164.772 kb on - strand, within Psyr_0151 at 164.772 kb on - strand, within Psyr_0151 at 164.772 kb on - strand, within Psyr_0151
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Paclitaxel 10 mM remove 162,037 + Psyr_0149 0.58 +1.0 162,258 + Psyr_0149 0.73 -0.6 162,259 - Psyr_0149 0.73 -0.5 162,597 + +0.7 162,597 + -0.4 162,598 - +0.3 162,598 - +0.4 162,608 + +0.5 162,680 - +1.1 162,763 - -0.0 162,763 - +1.1 162,927 + Psyr_0150 0.14 +0.8 162,928 - Psyr_0150 0.14 +1.8 162,928 - Psyr_0150 0.14 +1.2 163,076 + Psyr_0150 0.27 +3.5 163,076 + Psyr_0150 0.27 -1.3 163,077 - Psyr_0150 0.27 +0.0 163,217 + Psyr_0150 0.39 +0.4 163,218 - Psyr_0150 0.39 -2.3 163,218 - Psyr_0150 0.39 +0.9 163,218 - Psyr_0150 0.39 -0.5 163,218 - Psyr_0150 0.39 -0.3 163,227 + Psyr_0150 0.40 -1.3 163,227 + Psyr_0150 0.40 +1.0 163,227 + Psyr_0150 0.40 -0.9 163,457 + Psyr_0150 0.60 +0.5 163,458 - Psyr_0150 0.60 +0.4 163,458 - Psyr_0150 0.60 -1.5 163,629 + Psyr_0150 0.75 +0.9 163,629 + Psyr_0150 0.75 +0.8 163,630 - Psyr_0150 0.75 -0.1 163,630 - Psyr_0150 0.75 -0.1 163,630 - Psyr_0150 0.75 +1.3 163,630 - Psyr_0150 0.75 -0.5 163,833 + -1.8 163,834 - -0.6 163,834 - -2.7 163,926 + +1.0 163,927 - -2.2 164,087 + Psyr_0151 0.15 +2.2 164,087 + Psyr_0151 0.15 -2.0 164,087 + Psyr_0151 0.15 +0.0 164,087 + Psyr_0151 0.15 -0.0 164,088 - Psyr_0151 0.15 -0.8 164,088 - Psyr_0151 0.15 -0.3 164,088 - Psyr_0151 0.15 +1.1 164,088 - Psyr_0151 0.15 -0.2 164,088 - Psyr_0151 0.15 +1.0 164,088 - Psyr_0151 0.15 -0.1 164,088 - Psyr_0151 0.15 +0.5 164,250 - Psyr_0151 0.29 +0.5 164,330 + Psyr_0151 0.35 +2.5 164,330 + Psyr_0151 0.35 -1.3 164,330 + Psyr_0151 0.35 -0.6 164,330 + Psyr_0151 0.35 -0.4 164,331 - Psyr_0151 0.36 -1.4 164,666 + Psyr_0151 0.64 +1.1 164,667 - Psyr_0151 0.64 +0.3 164,729 + Psyr_0151 0.70 -0.9 164,729 + Psyr_0151 0.70 -4.1 164,729 + Psyr_0151 0.70 +0.5 164,729 + Psyr_0151 0.70 +0.2 164,729 + Psyr_0151 0.70 -1.9 164,729 + Psyr_0151 0.70 -0.3 164,730 - Psyr_0151 0.70 +0.5 164,771 + Psyr_0151 0.73 -1.4 164,771 + Psyr_0151 0.73 -0.7 164,772 - Psyr_0151 0.73 +0.0 164,772 - Psyr_0151 0.73 -0.9 164,772 - Psyr_0151 0.73 +1.0 164,772 - Psyr_0151 0.73 -0.3
Or see this region's nucleotide sequence