Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS23875 and HSERO_RS23880 are separated by 35 nucleotides HSERO_RS23880 and HSERO_RS23885 are separated by 217 nucleotides HSERO_RS23885 and HSERO_RS23890 are separated by 88 nucleotides HSERO_RS23890 and HSERO_RS23895 are separated by 271 nucleotides
HSERO_RS23875: HSERO_RS23875 - lipoate-protein ligase B, at 5,473,531 to 5,474,223
_RS23875
HSERO_RS23880: HSERO_RS23880 - hypothetical protein, at 5,474,259 to 5,474,615
_RS23880
HSERO_RS23885: HSERO_RS23885 - XRE family transcriptional regulator, at 5,474,833 to 5,475,753
_RS23885
HSERO_RS23890: HSERO_RS23890 - hypothetical protein, at 5,475,842 to 5,476,123
_RS23890
HSERO_RS23895: HSERO_RS23895 - amidophosphoribosyltransferase, at 5,476,395 to 5,476,973
_RS23895
Position (kb) 5474
5475
5476 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 5474.494 kb on + strand, within HSERO_RS23880 at 5474.512 kb on + strand, within HSERO_RS23880 at 5474.515 kb on - strand, within HSERO_RS23880 at 5474.520 kb on - strand, within HSERO_RS23880 at 5474.525 kb on - strand, within HSERO_RS23880 at 5474.530 kb on - strand, within HSERO_RS23880 at 5474.538 kb on + strand, within HSERO_RS23880 at 5474.541 kb on - strand, within HSERO_RS23880 at 5474.592 kb on + strand at 5474.593 kb on - strand at 5474.659 kb on + strand at 5474.673 kb on + strand at 5474.681 kb on - strand at 5474.767 kb on - strand at 5474.837 kb on - strand at 5474.934 kb on - strand, within HSERO_RS23885 at 5475.089 kb on + strand, within HSERO_RS23885 at 5475.159 kb on - strand, within HSERO_RS23885 at 5475.169 kb on - strand, within HSERO_RS23885 at 5475.268 kb on + strand, within HSERO_RS23885 at 5475.315 kb on + strand, within HSERO_RS23885 at 5475.464 kb on + strand, within HSERO_RS23885 at 5475.530 kb on - strand, within HSERO_RS23885 at 5475.537 kb on + strand, within HSERO_RS23885 at 5475.545 kb on - strand, within HSERO_RS23885 at 5476.287 kb on + strand at 5476.337 kb on + strand at 5476.461 kb on + strand, within HSERO_RS23895 at 5476.471 kb on + strand, within HSERO_RS23895 at 5476.499 kb on + strand, within HSERO_RS23895 at 5476.594 kb on - strand, within HSERO_RS23895 at 5476.700 kb on - strand, within HSERO_RS23895 at 5476.745 kb on + strand, within HSERO_RS23895
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 5,474,494 + HSERO_RS23880 0.66 +4.3 5,474,512 + HSERO_RS23880 0.71 -0.1 5,474,515 - HSERO_RS23880 0.72 -0.8 5,474,520 - HSERO_RS23880 0.73 +1.0 5,474,525 - HSERO_RS23880 0.75 -0.7 5,474,530 - HSERO_RS23880 0.76 +0.1 5,474,538 + HSERO_RS23880 0.78 -1.5 5,474,541 - HSERO_RS23880 0.79 -0.7 5,474,592 + -0.4 5,474,593 - +0.5 5,474,659 + +0.2 5,474,673 + +0.8 5,474,681 - +1.0 5,474,767 - -0.5 5,474,837 - +0.3 5,474,934 - HSERO_RS23885 0.11 +0.5 5,475,089 + HSERO_RS23885 0.28 -0.5 5,475,159 - HSERO_RS23885 0.35 -0.3 5,475,169 - HSERO_RS23885 0.36 +0.7 5,475,268 + HSERO_RS23885 0.47 +0.4 5,475,315 + HSERO_RS23885 0.52 +0.1 5,475,464 + HSERO_RS23885 0.69 +0.1 5,475,530 - HSERO_RS23885 0.76 +0.6 5,475,537 + HSERO_RS23885 0.76 +0.7 5,475,545 - HSERO_RS23885 0.77 -1.0 5,476,287 + -0.8 5,476,337 + +0.7 5,476,461 + HSERO_RS23895 0.11 -1.1 5,476,471 + HSERO_RS23895 0.13 +0.2 5,476,499 + HSERO_RS23895 0.18 -0.1 5,476,594 - HSERO_RS23895 0.34 -1.7 5,476,700 - HSERO_RS23895 0.53 +0.1 5,476,745 + HSERO_RS23895 0.60 +0.3
Or see this region's nucleotide sequence