Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS07015 and HSERO_RS07020 are separated by 145 nucleotides HSERO_RS07020 and HSERO_RS07025 are separated by 338 nucleotides HSERO_RS07025 and HSERO_RS07030 overlap by 4 nucleotides
HSERO_RS07015: HSERO_RS07015 - isocitrate dehydrogenase, at 1,598,205 to 1,599,461
_RS07015
HSERO_RS07020: HSERO_RS07020 - cold-shock protein, at 1,599,607 to 1,599,813
_RS07020
HSERO_RS07025: HSERO_RS07025 - ATP-dependent Clp protease ClpS, at 1,600,152 to 1,600,466
_RS07025
HSERO_RS07030: HSERO_RS07030 - ATP-dependent Clp protease ATP-binding protein, at 1,600,463 to 1,602,766
_RS07030
Position (kb) 1599
1600 Strain fitness (log2 ratio) -2
-1
0
1 at 1598.796 kb on - strand, within HSERO_RS07015 at 1599.022 kb on - strand, within HSERO_RS07015 at 1599.022 kb on - strand, within HSERO_RS07015 at 1599.166 kb on - strand, within HSERO_RS07015 at 1599.166 kb on - strand, within HSERO_RS07015 at 1599.180 kb on - strand, within HSERO_RS07015 at 1599.220 kb on - strand, within HSERO_RS07015 at 1599.245 kb on - strand, within HSERO_RS07015 at 1599.341 kb on - strand at 1599.404 kb on - strand at 1599.512 kb on - strand at 1599.758 kb on - strand, within HSERO_RS07020 at 1599.884 kb on + strand at 1599.888 kb on - strand at 1600.050 kb on - strand at 1600.102 kb on - strand at 1600.119 kb on + strand at 1600.127 kb on - strand at 1600.129 kb on + strand at 1600.137 kb on - strand at 1600.137 kb on - strand at 1600.192 kb on + strand, within HSERO_RS07025 at 1600.407 kb on + strand, within HSERO_RS07025
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,598,796 - HSERO_RS07015 0.47 +0.7 1,599,022 - HSERO_RS07015 0.65 -0.6 1,599,022 - HSERO_RS07015 0.65 +0.8 1,599,166 - HSERO_RS07015 0.76 -0.2 1,599,166 - HSERO_RS07015 0.76 -0.3 1,599,180 - HSERO_RS07015 0.78 -0.9 1,599,220 - HSERO_RS07015 0.81 -0.1 1,599,245 - HSERO_RS07015 0.83 +0.0 1,599,341 - -0.1 1,599,404 - +1.6 1,599,512 - +0.7 1,599,758 - HSERO_RS07020 0.73 -2.3 1,599,884 + +0.0 1,599,888 - +0.6 1,600,050 - +0.3 1,600,102 - -0.7 1,600,119 + -0.4 1,600,127 - -0.6 1,600,129 + -1.0 1,600,137 - -0.4 1,600,137 - -0.4 1,600,192 + HSERO_RS07025 0.13 -1.1 1,600,407 + HSERO_RS07025 0.81 -1.7
Or see this region's nucleotide sequence