Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT4463

Experiment: BHIS with Sulfaquinoxaline 0.1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT4461 and BT4462 are separated by 5 nucleotidesBT4462 and BT4463 are separated by 25 nucleotidesBT4463 and BT4464 overlap by 4 nucleotidesBT4464 and BT4465 are separated by 190 nucleotides BT4461: BT4461 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 5,871,960 to 5,872,532 BT4461 BT4462: BT4462 - conserved hypothetical protein (NCBI ptt file), at 5,872,538 to 5,873,191 BT4462 BT4463: BT4463 - phosphatidate cytidylyltransferase (NCBI ptt file), at 5,873,217 to 5,874,173 BT4463 BT4464: BT4464 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase-related protein (NCBI ptt file), at 5,874,170 to 5,874,823 BT4464 BT4465: BT4465 - conserved hypothetical protein, putative outer membrane protein (NCBI ptt file), at 5,875,014 to 5,876,879 BT4465 Position (kb) 5873 5874 5875Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 5872.411 kb on + strand, within BT4461at 5872.426 kb on + strand, within BT4461at 5872.435 kb on + strand, within BT4461at 5872.436 kb on - strand, within BT4461at 5872.472 kb on + strand, within BT4461at 5872.473 kb on - strand, within BT4461at 5872.519 kb on - strandat 5872.566 kb on + strandat 5872.566 kb on + strandat 5872.735 kb on + strand, within BT4462at 5872.975 kb on - strand, within BT4462at 5873.162 kb on - strandat 5873.162 kb on - strandat 5873.164 kb on - strandat 5873.165 kb on + strandat 5873.168 kb on + strandat 5873.221 kb on + strandat 5873.231 kb on - strandat 5873.313 kb on - strand, within BT4463at 5873.313 kb on - strand, within BT4463at 5873.313 kb on - strand, within BT4463at 5873.313 kb on - strand, within BT4463at 5873.362 kb on - strand, within BT4463at 5873.449 kb on + strand, within BT4463at 5873.449 kb on + strand, within BT4463at 5873.455 kb on - strand, within BT4463at 5873.494 kb on + strand, within BT4463at 5873.748 kb on + strand, within BT4463at 5873.749 kb on - strand, within BT4463at 5873.766 kb on + strand, within BT4463at 5873.777 kb on + strand, within BT4463at 5873.778 kb on - strand, within BT4463at 5873.778 kb on - strand, within BT4463at 5873.793 kb on + strand, within BT4463at 5873.800 kb on + strand, within BT4463at 5873.800 kb on + strand, within BT4463at 5873.821 kb on + strand, within BT4463at 5873.831 kb on - strand, within BT4463at 5874.000 kb on - strand, within BT4463at 5874.038 kb on + strand, within BT4463at 5874.039 kb on - strand, within BT4463at 5874.039 kb on - strand, within BT4463at 5874.117 kb on + strandat 5874.117 kb on + strandat 5874.118 kb on - strandat 5874.127 kb on - strandat 5874.144 kb on - strandat 5874.150 kb on + strandat 5874.151 kb on - strandat 5874.163 kb on - strandat 5874.216 kb on - strandat 5874.249 kb on - strand, within BT4464at 5874.251 kb on - strand, within BT4464at 5874.256 kb on + strand, within BT4464at 5874.257 kb on - strand, within BT4464at 5874.384 kb on + strand, within BT4464at 5874.471 kb on + strand, within BT4464at 5874.487 kb on + strand, within BT4464at 5874.528 kb on - strand, within BT4464at 5874.586 kb on + strand, within BT4464at 5874.587 kb on - strand, within BT4464at 5874.590 kb on + strand, within BT4464at 5874.591 kb on - strand, within BT4464at 5874.659 kb on - strand, within BT4464at 5874.659 kb on - strand, within BT4464at 5874.659 kb on - strand, within BT4464at 5874.701 kb on + strand, within BT4464at 5874.761 kb on - strandat 5874.850 kb on + strandat 5874.851 kb on - strandat 5874.853 kb on - strandat 5874.969 kb on + strandat 5875.000 kb on - strandat 5875.013 kb on + strandat 5875.013 kb on + strandat 5875.058 kb on - strandat 5875.065 kb on - strandat 5875.067 kb on + strandat 5875.067 kb on + strandat 5875.080 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Sulfaquinoxaline 0.1 mM
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5,872,411 + BT4461 0.79 -2.4
5,872,426 + BT4461 0.81 +1.1
5,872,435 + BT4461 0.83 -3.1
5,872,436 - BT4461 0.83 -0.3
5,872,472 + BT4461 0.89 -2.1
5,872,473 - BT4461 0.90 -2.1
5,872,519 - -0.9
5,872,566 + +1.4
5,872,566 + -0.4
5,872,735 + BT4462 0.30 -0.5
5,872,975 - BT4462 0.67 +0.1
5,873,162 - -0.9
5,873,162 - +1.7
5,873,164 - +0.1
5,873,165 + -2.8
5,873,168 + -2.8
5,873,221 + +0.7
5,873,231 - -2.1
5,873,313 - BT4463 0.10 +1.4
5,873,313 - BT4463 0.10 +1.1
5,873,313 - BT4463 0.10 -2.6
5,873,313 - BT4463 0.10 -2.7
5,873,362 - BT4463 0.15 -3.4
5,873,449 + BT4463 0.24 +0.7
5,873,449 + BT4463 0.24 -2.0
5,873,455 - BT4463 0.25 -3.2
5,873,494 + BT4463 0.29 -0.9
5,873,748 + BT4463 0.55 -2.2
5,873,749 - BT4463 0.56 -2.1
5,873,766 + BT4463 0.57 -1.5
5,873,777 + BT4463 0.59 -1.8
5,873,778 - BT4463 0.59 -0.5
5,873,778 - BT4463 0.59 -0.8
5,873,793 + BT4463 0.60 -4.0
5,873,800 + BT4463 0.61 -0.9
5,873,800 + BT4463 0.61 -1.9
5,873,821 + BT4463 0.63 -2.1
5,873,831 - BT4463 0.64 -0.5
5,874,000 - BT4463 0.82 -2.2
5,874,038 + BT4463 0.86 -0.5
5,874,039 - BT4463 0.86 +0.1
5,874,039 - BT4463 0.86 -1.7
5,874,117 + +0.4
5,874,117 + -1.5
5,874,118 - -2.7
5,874,127 - -1.6
5,874,144 - +1.4
5,874,150 + +1.1
5,874,151 - +0.7
5,874,163 - -0.2
5,874,216 - -1.1
5,874,249 - BT4464 0.12 +0.1
5,874,251 - BT4464 0.12 +1.1
5,874,256 + BT4464 0.13 +0.5
5,874,257 - BT4464 0.13 -0.9
5,874,384 + BT4464 0.33 -3.9
5,874,471 + BT4464 0.46 -2.2
5,874,487 + BT4464 0.48 +0.9
5,874,528 - BT4464 0.55 +0.1
5,874,586 + BT4464 0.64 -2.1
5,874,587 - BT4464 0.64 +0.4
5,874,590 + BT4464 0.64 -1.5
5,874,591 - BT4464 0.64 -2.2
5,874,659 - BT4464 0.75 +0.1
5,874,659 - BT4464 0.75 -1.5
5,874,659 - BT4464 0.75 +1.3
5,874,701 + BT4464 0.81 -1.0
5,874,761 - -2.1
5,874,850 + +0.1
5,874,851 - -1.0
5,874,853 - -1.8
5,874,969 + -1.2
5,875,000 - +0.1
5,875,013 + -2.2
5,875,013 + -0.9
5,875,058 - +0.1
5,875,065 - -0.3
5,875,067 + +1.7
5,875,067 + -0.9
5,875,080 - -2.7

Or see this region's nucleotide sequence