Experiment: BHIS with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4461 and BT4462 are separated by 5 nucleotides BT4462 and BT4463 are separated by 25 nucleotides BT4463 and BT4464 overlap by 4 nucleotides BT4464 and BT4465 are separated by 190 nucleotides
BT4461: BT4461 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 5,871,960 to 5,872,532
BT4461
BT4462: BT4462 - conserved hypothetical protein (NCBI ptt file), at 5,872,538 to 5,873,191
BT4462
BT4463: BT4463 - phosphatidate cytidylyltransferase (NCBI ptt file), at 5,873,217 to 5,874,173
BT4463
BT4464: BT4464 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase-related protein (NCBI ptt file), at 5,874,170 to 5,874,823
BT4464
BT4465: BT4465 - conserved hypothetical protein, putative outer membrane protein (NCBI ptt file), at 5,875,014 to 5,876,879
BT4465
Position (kb)
5873
5874
5875 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5872.411 kb on + strand, within BT4461 at 5872.426 kb on + strand, within BT4461 at 5872.435 kb on + strand, within BT4461 at 5872.436 kb on - strand, within BT4461 at 5872.472 kb on + strand, within BT4461 at 5872.473 kb on - strand, within BT4461 at 5872.519 kb on - strand at 5872.566 kb on + strand at 5872.566 kb on + strand at 5872.735 kb on + strand, within BT4462 at 5872.975 kb on - strand, within BT4462 at 5873.162 kb on - strand at 5873.162 kb on - strand at 5873.164 kb on - strand at 5873.165 kb on + strand at 5873.168 kb on + strand at 5873.221 kb on + strand at 5873.231 kb on - strand at 5873.313 kb on - strand, within BT4463 at 5873.313 kb on - strand, within BT4463 at 5873.313 kb on - strand, within BT4463 at 5873.313 kb on - strand, within BT4463 at 5873.362 kb on - strand, within BT4463 at 5873.449 kb on + strand, within BT4463 at 5873.449 kb on + strand, within BT4463 at 5873.455 kb on - strand, within BT4463 at 5873.494 kb on + strand, within BT4463 at 5873.748 kb on + strand, within BT4463 at 5873.749 kb on - strand, within BT4463 at 5873.766 kb on + strand, within BT4463 at 5873.777 kb on + strand, within BT4463 at 5873.778 kb on - strand, within BT4463 at 5873.778 kb on - strand, within BT4463 at 5873.793 kb on + strand, within BT4463 at 5873.800 kb on + strand, within BT4463 at 5873.800 kb on + strand, within BT4463 at 5873.821 kb on + strand, within BT4463 at 5873.831 kb on - strand, within BT4463 at 5874.000 kb on - strand, within BT4463 at 5874.038 kb on + strand, within BT4463 at 5874.039 kb on - strand, within BT4463 at 5874.039 kb on - strand, within BT4463 at 5874.117 kb on + strand at 5874.117 kb on + strand at 5874.118 kb on - strand at 5874.127 kb on - strand at 5874.144 kb on - strand at 5874.150 kb on + strand at 5874.151 kb on - strand at 5874.163 kb on - strand at 5874.216 kb on - strand at 5874.249 kb on - strand, within BT4464 at 5874.251 kb on - strand, within BT4464 at 5874.256 kb on + strand, within BT4464 at 5874.257 kb on - strand, within BT4464 at 5874.384 kb on + strand, within BT4464 at 5874.471 kb on + strand, within BT4464 at 5874.487 kb on + strand, within BT4464 at 5874.528 kb on - strand, within BT4464 at 5874.586 kb on + strand, within BT4464 at 5874.587 kb on - strand, within BT4464 at 5874.590 kb on + strand, within BT4464 at 5874.591 kb on - strand, within BT4464 at 5874.659 kb on - strand, within BT4464 at 5874.659 kb on - strand, within BT4464 at 5874.659 kb on - strand, within BT4464 at 5874.701 kb on + strand, within BT4464 at 5874.761 kb on - strand at 5874.850 kb on + strand at 5874.851 kb on - strand at 5874.853 kb on - strand at 5874.969 kb on + strand at 5875.000 kb on - strand at 5875.013 kb on + strand at 5875.013 kb on + strand at 5875.058 kb on - strand at 5875.065 kb on - strand at 5875.067 kb on + strand at 5875.067 kb on + strand at 5875.080 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Sulfaquinoxaline 0.1 mM remove 5,872,411 + BT4461 0.79 -2.4 5,872,426 + BT4461 0.81 +1.1 5,872,435 + BT4461 0.83 -3.1 5,872,436 - BT4461 0.83 -0.3 5,872,472 + BT4461 0.89 -2.1 5,872,473 - BT4461 0.90 -2.1 5,872,519 - -0.9 5,872,566 + +1.4 5,872,566 + -0.4 5,872,735 + BT4462 0.30 -0.5 5,872,975 - BT4462 0.67 +0.1 5,873,162 - -0.9 5,873,162 - +1.7 5,873,164 - +0.1 5,873,165 + -2.8 5,873,168 + -2.8 5,873,221 + +0.7 5,873,231 - -2.1 5,873,313 - BT4463 0.10 +1.4 5,873,313 - BT4463 0.10 +1.1 5,873,313 - BT4463 0.10 -2.6 5,873,313 - BT4463 0.10 -2.7 5,873,362 - BT4463 0.15 -3.4 5,873,449 + BT4463 0.24 +0.7 5,873,449 + BT4463 0.24 -2.0 5,873,455 - BT4463 0.25 -3.2 5,873,494 + BT4463 0.29 -0.9 5,873,748 + BT4463 0.55 -2.2 5,873,749 - BT4463 0.56 -2.1 5,873,766 + BT4463 0.57 -1.5 5,873,777 + BT4463 0.59 -1.8 5,873,778 - BT4463 0.59 -0.5 5,873,778 - BT4463 0.59 -0.8 5,873,793 + BT4463 0.60 -4.0 5,873,800 + BT4463 0.61 -0.9 5,873,800 + BT4463 0.61 -1.9 5,873,821 + BT4463 0.63 -2.1 5,873,831 - BT4463 0.64 -0.5 5,874,000 - BT4463 0.82 -2.2 5,874,038 + BT4463 0.86 -0.5 5,874,039 - BT4463 0.86 +0.1 5,874,039 - BT4463 0.86 -1.7 5,874,117 + +0.4 5,874,117 + -1.5 5,874,118 - -2.7 5,874,127 - -1.6 5,874,144 - +1.4 5,874,150 + +1.1 5,874,151 - +0.7 5,874,163 - -0.2 5,874,216 - -1.1 5,874,249 - BT4464 0.12 +0.1 5,874,251 - BT4464 0.12 +1.1 5,874,256 + BT4464 0.13 +0.5 5,874,257 - BT4464 0.13 -0.9 5,874,384 + BT4464 0.33 -3.9 5,874,471 + BT4464 0.46 -2.2 5,874,487 + BT4464 0.48 +0.9 5,874,528 - BT4464 0.55 +0.1 5,874,586 + BT4464 0.64 -2.1 5,874,587 - BT4464 0.64 +0.4 5,874,590 + BT4464 0.64 -1.5 5,874,591 - BT4464 0.64 -2.2 5,874,659 - BT4464 0.75 +0.1 5,874,659 - BT4464 0.75 -1.5 5,874,659 - BT4464 0.75 +1.3 5,874,701 + BT4464 0.81 -1.0 5,874,761 - -2.1 5,874,850 + +0.1 5,874,851 - -1.0 5,874,853 - -1.8 5,874,969 + -1.2 5,875,000 - +0.1 5,875,013 + -2.2 5,875,013 + -0.9 5,875,058 - +0.1 5,875,065 - -0.3 5,875,067 + +1.7 5,875,067 + -0.9 5,875,080 - -2.7
Or see this region's nucleotide sequence