Experiment: BHIS with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1326 and BT1327 are separated by 30 nucleotides BT1327 and BT1328 are separated by 116 nucleotides BT1328 and BT1329 are separated by 126 nucleotides BT1329 and BT1330 are separated by 136 nucleotides
BT1326: BT1326 - TPR-domain containing protein (NCBI ptt file), at 1,652,085 to 1,653,521
BT1326
BT1327: BT1327 - putative alkaline phosphatase (NCBI ptt file), at 1,653,552 to 1,654,190
BT1327
BT1328: BT1328 - hypothetical protein (NCBI ptt file), at 1,654,307 to 1,655,191
BT1328
BT1329: BT1329 - putative thiol peroxidase (NCBI ptt file), at 1,655,318 to 1,655,821
BT1329
BT1330: BT1330 - conserved hypothetical protein (NCBI ptt file), at 1,655,958 to 1,656,521
BT1330
Position (kb)
1654
1655
1656 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1653.419 kb on + strand at 1653.519 kb on + strand at 1654.206 kb on + strand at 1654.210 kb on - strand at 1654.210 kb on - strand at 1654.265 kb on - strand at 1654.268 kb on + strand at 1654.276 kb on - strand at 1654.278 kb on + strand at 1654.290 kb on - strand at 1654.411 kb on + strand, within BT1328 at 1654.412 kb on - strand, within BT1328 at 1654.415 kb on - strand, within BT1328 at 1654.530 kb on + strand, within BT1328 at 1654.532 kb on + strand at 1654.579 kb on + strand, within BT1328 at 1654.580 kb on - strand, within BT1328 at 1654.617 kb on - strand, within BT1328 at 1654.688 kb on + strand, within BT1328 at 1654.716 kb on + strand, within BT1328 at 1654.720 kb on + strand, within BT1328 at 1654.721 kb on - strand, within BT1328 at 1654.756 kb on + strand, within BT1328 at 1654.792 kb on - strand, within BT1328 at 1654.797 kb on + strand, within BT1328 at 1654.870 kb on - strand, within BT1328 at 1654.965 kb on - strand, within BT1328 at 1654.966 kb on + strand, within BT1328 at 1654.966 kb on + strand, within BT1328 at 1654.967 kb on - strand, within BT1328 at 1654.982 kb on - strand, within BT1328 at 1654.999 kb on + strand, within BT1328 at 1655.219 kb on + strand at 1655.230 kb on + strand at 1655.233 kb on + strand at 1655.238 kb on - strand at 1655.244 kb on + strand at 1655.266 kb on + strand at 1655.269 kb on + strand at 1655.269 kb on + strand at 1655.292 kb on - strand at 1655.312 kb on + strand at 1655.315 kb on + strand at 1655.320 kb on - strand at 1655.393 kb on + strand, within BT1329 at 1655.394 kb on - strand, within BT1329 at 1655.472 kb on + strand, within BT1329 at 1655.620 kb on + strand, within BT1329 at 1655.672 kb on - strand, within BT1329 at 1655.735 kb on + strand, within BT1329 at 1655.736 kb on - strand, within BT1329 at 1655.763 kb on - strand, within BT1329 at 1655.763 kb on - strand, within BT1329 at 1655.936 kb on - strand at 1655.936 kb on - strand at 1655.957 kb on + strand at 1655.968 kb on + strand at 1656.053 kb on + strand, within BT1330 at 1656.103 kb on + strand, within BT1330 at 1656.180 kb on + strand, within BT1330
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Sulfaquinoxaline 0.1 mM remove 1,653,419 + -0.5 1,653,519 + -2.2 1,654,206 + -1.7 1,654,210 - -1.9 1,654,210 - -2.3 1,654,265 - -0.9 1,654,268 + -1.0 1,654,276 - -1.9 1,654,278 + -1.8 1,654,290 - -1.9 1,654,411 + BT1328 0.12 -1.9 1,654,412 - BT1328 0.12 -1.5 1,654,415 - BT1328 0.12 -0.9 1,654,530 + BT1328 0.25 -2.3 1,654,532 + +0.1 1,654,579 + BT1328 0.31 -2.6 1,654,580 - BT1328 0.31 -2.5 1,654,617 - BT1328 0.35 -1.9 1,654,688 + BT1328 0.43 +0.1 1,654,716 + BT1328 0.46 -2.5 1,654,720 + BT1328 0.47 -2.8 1,654,721 - BT1328 0.47 +3.1 1,654,756 + BT1328 0.51 -0.9 1,654,792 - BT1328 0.55 -0.9 1,654,797 + BT1328 0.55 -0.4 1,654,870 - BT1328 0.64 +0.6 1,654,965 - BT1328 0.74 -2.8 1,654,966 + BT1328 0.74 -1.5 1,654,966 + BT1328 0.74 -1.8 1,654,967 - BT1328 0.75 -1.9 1,654,982 - BT1328 0.76 -2.8 1,654,999 + BT1328 0.78 -2.5 1,655,219 + -3.6 1,655,230 + -2.2 1,655,233 + -0.9 1,655,238 - -1.8 1,655,244 + -1.3 1,655,266 + +0.1 1,655,269 + -1.7 1,655,269 + -0.3 1,655,292 - +1.6 1,655,312 + -1.8 1,655,315 + -0.9 1,655,320 - -0.8 1,655,393 + BT1329 0.15 +1.8 1,655,394 - BT1329 0.15 -0.9 1,655,472 + BT1329 0.31 +0.4 1,655,620 + BT1329 0.60 -2.8 1,655,672 - BT1329 0.70 -1.5 1,655,735 + BT1329 0.83 -0.5 1,655,736 - BT1329 0.83 +1.1 1,655,763 - BT1329 0.88 -1.3 1,655,763 - BT1329 0.88 +0.1 1,655,936 - -4.0 1,655,936 - +0.1 1,655,957 + -4.5 1,655,968 + +0.1 1,656,053 + BT1330 0.17 -1.8 1,656,103 + BT1330 0.26 -0.4 1,656,180 + BT1330 0.39 +0.1
Or see this region's nucleotide sequence