Experiment: BHIS with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0211 and BT0212 are separated by 50 nucleotides BT0212 and BT0213 are separated by 12 nucleotides BT0213 and BT0214 are separated by 21 nucleotides
BT0211: BT0211 - hypothetical protein (NCBI ptt file), at 232,865 to 234,847
BT0211
BT0212: BT0212 - protease (NCBI ptt file), at 234,898 to 236,985
BT0212
BT0213: BT0213 - hypothetical protein (NCBI ptt file), at 236,998 to 237,813
BT0213
BT0214: BT0214 - hypothetical protein (NCBI ptt file), at 237,835 to 238,311
BT0214
Position (kb)
234
235
236
237 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 233.927 kb on - strand, within BT0211 at 233.930 kb on - strand, within BT0211 at 233.998 kb on - strand, within BT0211 at 234.084 kb on + strand, within BT0211 at 234.084 kb on + strand, within BT0211 at 234.089 kb on - strand, within BT0211 at 234.089 kb on - strand, within BT0211 at 234.200 kb on + strand, within BT0211 at 234.311 kb on + strand, within BT0211 at 234.451 kb on + strand, within BT0211 at 234.506 kb on + strand, within BT0211 at 234.507 kb on - strand, within BT0211 at 234.618 kb on + strand, within BT0211 at 234.661 kb on - strand at 234.729 kb on + strand at 234.855 kb on - strand at 234.918 kb on + strand at 235.168 kb on + strand, within BT0212 at 235.182 kb on + strand, within BT0212 at 235.185 kb on - strand, within BT0212 at 235.300 kb on - strand, within BT0212 at 235.376 kb on - strand, within BT0212 at 235.416 kb on + strand, within BT0212 at 235.459 kb on + strand, within BT0212 at 235.488 kb on + strand, within BT0212 at 235.511 kb on + strand, within BT0212 at 235.588 kb on - strand, within BT0212 at 235.588 kb on - strand, within BT0212 at 235.622 kb on - strand, within BT0212 at 235.622 kb on - strand, within BT0212 at 235.648 kb on - strand, within BT0212 at 235.676 kb on - strand, within BT0212 at 235.683 kb on + strand, within BT0212 at 235.684 kb on - strand, within BT0212 at 235.717 kb on - strand, within BT0212 at 235.726 kb on + strand, within BT0212 at 235.726 kb on + strand, within BT0212 at 235.727 kb on - strand, within BT0212 at 235.727 kb on - strand, within BT0212 at 235.803 kb on + strand, within BT0212 at 235.887 kb on + strand, within BT0212 at 236.032 kb on + strand, within BT0212 at 236.217 kb on + strand, within BT0212 at 236.261 kb on - strand, within BT0212 at 236.262 kb on + strand, within BT0212 at 236.263 kb on - strand, within BT0212 at 236.431 kb on - strand, within BT0212 at 236.437 kb on - strand, within BT0212 at 236.482 kb on + strand, within BT0212 at 236.482 kb on + strand, within BT0212 at 236.593 kb on + strand, within BT0212 at 236.701 kb on - strand, within BT0212 at 236.727 kb on - strand, within BT0212 at 236.737 kb on - strand, within BT0212 at 236.740 kb on + strand, within BT0212 at 236.741 kb on - strand, within BT0212 at 236.804 kb on + strand at 236.805 kb on - strand at 236.810 kb on - strand at 236.829 kb on + strand at 236.940 kb on + strand at 236.964 kb on + strand at 236.992 kb on + strand at 237.033 kb on - strand at 237.039 kb on - strand at 237.145 kb on - strand, within BT0213 at 237.228 kb on + strand, within BT0213 at 237.233 kb on + strand, within BT0213 at 237.234 kb on - strand, within BT0213 at 237.234 kb on - strand, within BT0213 at 237.271 kb on - strand, within BT0213 at 237.311 kb on + strand, within BT0213 at 237.347 kb on - strand, within BT0213 at 237.437 kb on + strand, within BT0213 at 237.444 kb on + strand, within BT0213 at 237.445 kb on - strand, within BT0213 at 237.485 kb on - strand, within BT0213 at 237.688 kb on - strand, within BT0213 at 237.692 kb on + strand, within BT0213 at 237.745 kb on - strand at 237.747 kb on + strand at 237.804 kb on + strand at 237.804 kb on + strand at 237.807 kb on + strand at 237.818 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Sulfaquinoxaline 0.1 mM remove 233,927 - BT0211 0.54 -1.6 233,930 - BT0211 0.54 +0.7 233,998 - BT0211 0.57 +2.1 234,084 + BT0211 0.61 +0.1 234,084 + BT0211 0.61 -1.3 234,089 - BT0211 0.62 -2.3 234,089 - BT0211 0.62 +0.1 234,200 + BT0211 0.67 +1.1 234,311 + BT0211 0.73 -2.6 234,451 + BT0211 0.80 -1.1 234,506 + BT0211 0.83 -0.9 234,507 - BT0211 0.83 -0.9 234,618 + BT0211 0.88 -2.3 234,661 - -1.6 234,729 + -1.4 234,855 - -0.7 234,918 + -3.3 235,168 + BT0212 0.13 -0.9 235,182 + BT0212 0.14 -0.4 235,185 - BT0212 0.14 +0.1 235,300 - BT0212 0.19 +1.1 235,376 - BT0212 0.23 +2.1 235,416 + BT0212 0.25 -1.4 235,459 + BT0212 0.27 +0.1 235,488 + BT0212 0.28 -0.9 235,511 + BT0212 0.29 -1.9 235,588 - BT0212 0.33 -2.0 235,588 - BT0212 0.33 +1.7 235,622 - BT0212 0.35 -3.2 235,622 - BT0212 0.35 -2.2 235,648 - BT0212 0.36 +0.1 235,676 - BT0212 0.37 +0.9 235,683 + BT0212 0.38 +1.7 235,684 - BT0212 0.38 +1.7 235,717 - BT0212 0.39 -1.3 235,726 + BT0212 0.40 -0.9 235,726 + BT0212 0.40 -1.3 235,727 - BT0212 0.40 -1.6 235,727 - BT0212 0.40 -0.6 235,803 + BT0212 0.43 +1.1 235,887 + BT0212 0.47 -4.6 236,032 + BT0212 0.54 +1.1 236,217 + BT0212 0.63 -1.4 236,261 - BT0212 0.65 -0.4 236,262 + BT0212 0.65 +1.7 236,263 - BT0212 0.65 +2.1 236,431 - BT0212 0.73 +0.5 236,437 - BT0212 0.74 -0.9 236,482 + BT0212 0.76 +0.1 236,482 + BT0212 0.76 -0.6 236,593 + BT0212 0.81 +1.7 236,701 - BT0212 0.86 +0.5 236,727 - BT0212 0.88 +0.1 236,737 - BT0212 0.88 +1.1 236,740 + BT0212 0.88 -1.4 236,741 - BT0212 0.88 +1.1 236,804 + -0.9 236,805 - -2.7 236,810 - +0.1 236,829 + -2.2 236,940 + -0.9 236,964 + -1.4 236,992 + +0.1 237,033 - -1.3 237,039 - -2.3 237,145 - BT0213 0.18 -1.4 237,228 + BT0213 0.28 -3.2 237,233 + BT0213 0.29 -2.2 237,234 - BT0213 0.29 -1.9 237,234 - BT0213 0.29 +2.1 237,271 - BT0213 0.33 -0.2 237,311 + BT0213 0.38 -2.2 237,347 - BT0213 0.43 -1.4 237,437 + BT0213 0.54 -1.7 237,444 + BT0213 0.55 -2.3 237,445 - BT0213 0.55 +0.7 237,485 - BT0213 0.60 +0.1 237,688 - BT0213 0.85 -0.6 237,692 + BT0213 0.85 -3.3 237,745 - -2.3 237,747 + -1.7 237,804 + -2.2 237,804 + +0.1 237,807 + -0.9 237,818 - +0.1
Or see this region's nucleotide sequence