Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4680 and Psyr_4681 are separated by 590 nucleotides Psyr_4681 and Psyr_4682 are separated by 333 nucleotides Psyr_4682 and Psyr_4683 are separated by 153 nucleotides
Psyr_4680: Psyr_4680 - Integrase, catalytic region, at 5,547,340 to 5,548,176
_4680
Psyr_4681: Psyr_4681 - Acyl-CoA dehydrogenase, C-terminal:Acyl-CoA dehydrogenase, central region, at 5,548,767 to 5,550,572
_4681
Psyr_4682: Psyr_4682 - conserved hypothetical protein, at 5,550,906 to 5,551,202
_4682
Psyr_4683: Psyr_4683 - dethiobiotin synthase, at 5,551,356 to 5,552,036
_4683
Position (kb)
5548
5549
5550
5551 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5548.182 kb on + strand at 5548.294 kb on - strand at 5548.294 kb on - strand at 5548.315 kb on - strand at 5548.315 kb on - strand at 5548.315 kb on - strand at 5548.585 kb on + strand at 5548.585 kb on + strand at 5548.586 kb on - strand at 5548.586 kb on - strand at 5548.688 kb on + strand at 5548.688 kb on + strand at 5548.768 kb on + strand at 5548.768 kb on + strand at 5548.769 kb on - strand at 5548.771 kb on + strand at 5548.771 kb on + strand at 5548.772 kb on - strand at 5548.772 kb on - strand at 5548.873 kb on + strand at 5548.873 kb on + strand at 5548.873 kb on + strand at 5548.973 kb on - strand, within Psyr_4681 at 5548.973 kb on - strand, within Psyr_4681 at 5548.987 kb on + strand, within Psyr_4681 at 5549.005 kb on + strand, within Psyr_4681 at 5549.005 kb on + strand, within Psyr_4681 at 5549.005 kb on + strand, within Psyr_4681 at 5549.005 kb on + strand, within Psyr_4681 at 5549.006 kb on - strand, within Psyr_4681 at 5549.006 kb on - strand, within Psyr_4681 at 5549.006 kb on - strand, within Psyr_4681 at 5549.006 kb on - strand, within Psyr_4681 at 5549.006 kb on - strand, within Psyr_4681 at 5549.006 kb on - strand, within Psyr_4681 at 5549.006 kb on - strand, within Psyr_4681 at 5549.006 kb on - strand, within Psyr_4681 at 5549.006 kb on - strand, within Psyr_4681 at 5549.254 kb on + strand, within Psyr_4681 at 5549.254 kb on + strand, within Psyr_4681 at 5549.254 kb on + strand, within Psyr_4681 at 5549.255 kb on - strand, within Psyr_4681 at 5549.255 kb on - strand, within Psyr_4681 at 5549.326 kb on + strand, within Psyr_4681 at 5549.326 kb on + strand, within Psyr_4681 at 5549.326 kb on + strand, within Psyr_4681 at 5549.326 kb on + strand, within Psyr_4681 at 5549.327 kb on - strand, within Psyr_4681 at 5549.327 kb on - strand, within Psyr_4681 at 5549.446 kb on + strand, within Psyr_4681 at 5549.446 kb on + strand, within Psyr_4681 at 5549.447 kb on - strand, within Psyr_4681 at 5549.479 kb on + strand, within Psyr_4681 at 5549.479 kb on + strand, within Psyr_4681 at 5549.479 kb on + strand, within Psyr_4681 at 5549.480 kb on - strand, within Psyr_4681 at 5549.480 kb on - strand, within Psyr_4681 at 5549.480 kb on - strand, within Psyr_4681 at 5549.480 kb on - strand, within Psyr_4681 at 5549.623 kb on + strand, within Psyr_4681 at 5549.624 kb on - strand, within Psyr_4681 at 5549.624 kb on - strand, within Psyr_4681 at 5549.624 kb on - strand, within Psyr_4681 at 5549.624 kb on - strand, within Psyr_4681 at 5550.007 kb on + strand, within Psyr_4681 at 5550.007 kb on + strand, within Psyr_4681 at 5550.008 kb on - strand, within Psyr_4681 at 5550.008 kb on - strand, within Psyr_4681 at 5550.008 kb on - strand, within Psyr_4681 at 5550.008 kb on - strand, within Psyr_4681 at 5550.008 kb on - strand, within Psyr_4681 at 5550.008 kb on - strand, within Psyr_4681 at 5550.008 kb on - strand, within Psyr_4681 at 5550.008 kb on - strand, within Psyr_4681 at 5550.008 kb on - strand, within Psyr_4681 at 5550.124 kb on + strand, within Psyr_4681 at 5550.124 kb on + strand, within Psyr_4681 at 5550.125 kb on - strand, within Psyr_4681 at 5550.194 kb on - strand, within Psyr_4681 at 5550.325 kb on + strand, within Psyr_4681 at 5550.326 kb on - strand, within Psyr_4681 at 5550.326 kb on - strand, within Psyr_4681 at 5550.326 kb on - strand, within Psyr_4681 at 5550.512 kb on - strand at 5550.562 kb on + strand at 5550.562 kb on + strand at 5550.562 kb on + strand at 5550.562 kb on + strand at 5550.562 kb on + strand at 5550.562 kb on + strand at 5550.563 kb on - strand at 5550.563 kb on - strand at 5550.563 kb on - strand at 5550.572 kb on + strand at 5550.573 kb on - strand at 5550.655 kb on + strand at 5550.655 kb on + strand at 5550.655 kb on + strand at 5550.655 kb on + strand at 5550.655 kb on + strand at 5550.655 kb on + strand at 5550.655 kb on + strand at 5550.656 kb on - strand at 5550.656 kb on - strand at 5550.687 kb on + strand at 5550.687 kb on + strand at 5550.693 kb on + strand at 5550.713 kb on - strand at 5550.713 kb on - strand at 5550.748 kb on + strand at 5550.749 kb on - strand at 5550.763 kb on + strand at 5550.763 kb on + strand at 5550.763 kb on + strand at 5550.764 kb on - strand at 5550.764 kb on - strand at 5550.789 kb on + strand at 5550.801 kb on + strand at 5550.913 kb on + strand at 5550.913 kb on + strand at 5550.914 kb on - strand at 5550.914 kb on - strand at 5550.914 kb on - strand at 5550.914 kb on - strand at 5550.914 kb on - strand at 5550.914 kb on - strand at 5551.007 kb on + strand, within Psyr_4682 at 5551.007 kb on + strand, within Psyr_4682 at 5551.008 kb on - strand, within Psyr_4682 at 5551.202 kb on + strand at 5551.202 kb on + strand at 5551.263 kb on - strand at 5551.278 kb on + strand at 5551.345 kb on + strand at 5551.381 kb on + strand at 5551.382 kb on - strand at 5551.530 kb on + strand, within Psyr_4683 at 5551.530 kb on + strand, within Psyr_4683 at 5551.531 kb on - strand, within Psyr_4683
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 5,548,182 + +2.6 5,548,294 - -0.2 5,548,294 - -0.0 5,548,315 - +0.4 5,548,315 - +0.7 5,548,315 - -0.4 5,548,585 + -1.7 5,548,585 + -0.4 5,548,586 - +0.5 5,548,586 - -0.6 5,548,688 + +0.6 5,548,688 + -1.3 5,548,768 + +0.3 5,548,768 + -1.6 5,548,769 - +0.7 5,548,771 + -0.2 5,548,771 + -0.1 5,548,772 - -0.7 5,548,772 - +0.2 5,548,873 + -0.5 5,548,873 + +0.3 5,548,873 + +0.8 5,548,973 - Psyr_4681 0.11 -1.6 5,548,973 - Psyr_4681 0.11 -0.8 5,548,987 + Psyr_4681 0.12 -1.1 5,549,005 + Psyr_4681 0.13 -1.1 5,549,005 + Psyr_4681 0.13 -2.6 5,549,005 + Psyr_4681 0.13 -0.8 5,549,005 + Psyr_4681 0.13 +1.2 5,549,006 - Psyr_4681 0.13 +2.9 5,549,006 - Psyr_4681 0.13 -0.9 5,549,006 - Psyr_4681 0.13 -0.8 5,549,006 - Psyr_4681 0.13 +0.3 5,549,006 - Psyr_4681 0.13 -0.6 5,549,006 - Psyr_4681 0.13 +0.1 5,549,006 - Psyr_4681 0.13 +0.9 5,549,006 - Psyr_4681 0.13 -0.5 5,549,006 - Psyr_4681 0.13 -2.5 5,549,254 + Psyr_4681 0.27 -0.7 5,549,254 + Psyr_4681 0.27 -0.7 5,549,254 + Psyr_4681 0.27 -0.6 5,549,255 - Psyr_4681 0.27 -0.3 5,549,255 - Psyr_4681 0.27 +0.3 5,549,326 + Psyr_4681 0.31 +1.3 5,549,326 + Psyr_4681 0.31 -0.0 5,549,326 + Psyr_4681 0.31 +1.9 5,549,326 + Psyr_4681 0.31 +0.5 5,549,327 - Psyr_4681 0.31 -2.5 5,549,327 - Psyr_4681 0.31 -0.1 5,549,446 + Psyr_4681 0.38 -1.7 5,549,446 + Psyr_4681 0.38 -0.7 5,549,447 - Psyr_4681 0.38 +0.9 5,549,479 + Psyr_4681 0.39 +1.5 5,549,479 + Psyr_4681 0.39 +0.2 5,549,479 + Psyr_4681 0.39 -0.3 5,549,480 - Psyr_4681 0.39 -0.7 5,549,480 - Psyr_4681 0.39 +0.7 5,549,480 - Psyr_4681 0.39 +1.6 5,549,480 - Psyr_4681 0.39 -1.3 5,549,623 + Psyr_4681 0.47 -1.4 5,549,624 - Psyr_4681 0.47 +1.3 5,549,624 - Psyr_4681 0.47 -0.3 5,549,624 - Psyr_4681 0.47 -1.6 5,549,624 - Psyr_4681 0.47 +0.3 5,550,007 + Psyr_4681 0.69 -1.6 5,550,007 + Psyr_4681 0.69 -0.3 5,550,008 - Psyr_4681 0.69 -0.4 5,550,008 - Psyr_4681 0.69 -0.7 5,550,008 - Psyr_4681 0.69 +1.0 5,550,008 - Psyr_4681 0.69 -0.1 5,550,008 - Psyr_4681 0.69 +2.1 5,550,008 - Psyr_4681 0.69 -1.5 5,550,008 - Psyr_4681 0.69 -0.8 5,550,008 - Psyr_4681 0.69 -0.4 5,550,008 - Psyr_4681 0.69 -1.0 5,550,124 + Psyr_4681 0.75 -0.4 5,550,124 + Psyr_4681 0.75 -0.8 5,550,125 - Psyr_4681 0.75 +0.6 5,550,194 - Psyr_4681 0.79 +0.7 5,550,325 + Psyr_4681 0.86 +1.9 5,550,326 - Psyr_4681 0.86 -0.9 5,550,326 - Psyr_4681 0.86 +0.5 5,550,326 - Psyr_4681 0.86 +0.8 5,550,512 - +0.0 5,550,562 + -2.1 5,550,562 + -0.1 5,550,562 + -0.7 5,550,562 + -0.1 5,550,562 + +0.6 5,550,562 + +0.1 5,550,563 - -1.6 5,550,563 - +0.2 5,550,563 - +0.7 5,550,572 + -0.3 5,550,573 - +1.5 5,550,655 + -0.4 5,550,655 + +0.2 5,550,655 + -1.0 5,550,655 + -1.2 5,550,655 + +2.3 5,550,655 + -1.7 5,550,655 + -0.1 5,550,656 - +0.5 5,550,656 - -0.0 5,550,687 + -2.0 5,550,687 + +0.8 5,550,693 + -1.9 5,550,713 - -0.5 5,550,713 - +0.2 5,550,748 + -1.0 5,550,749 - -0.9 5,550,763 + -1.3 5,550,763 + -0.9 5,550,763 + +0.2 5,550,764 - -1.9 5,550,764 - +0.0 5,550,789 + -1.0 5,550,801 + -0.3 5,550,913 + -1.9 5,550,913 + -1.2 5,550,914 - -0.7 5,550,914 - -1.7 5,550,914 - -1.0 5,550,914 - +0.3 5,550,914 - -0.4 5,550,914 - -1.5 5,551,007 + Psyr_4682 0.34 -2.1 5,551,007 + Psyr_4682 0.34 -0.3 5,551,008 - Psyr_4682 0.34 -1.6 5,551,202 + -2.9 5,551,202 + +1.0 5,551,263 - -1.0 5,551,278 + -0.6 5,551,345 + -0.8 5,551,381 + -2.3 5,551,382 - -1.6 5,551,530 + Psyr_4683 0.26 -3.2 5,551,530 + Psyr_4683 0.26 -3.6 5,551,531 - Psyr_4683 0.26 -3.0
Or see this region's nucleotide sequence