Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4298 and Psyr_4299 are separated by 167 nucleotides Psyr_4299 and Psyr_4300 are separated by 4 nucleotides Psyr_4300 and Psyr_4301 are separated by 18 nucleotides Psyr_4301 and Psyr_4302 are separated by 280 nucleotides
Psyr_4298: Psyr_4298 - Protein of unknown function DUF6, at 5,125,227 to 5,126,141
_4298
Psyr_4299: Psyr_4299 - transcriptional regulator, AsnC family, at 5,126,309 to 5,126,776
_4299
Psyr_4300: Psyr_4300 - DNA polymerase III, epsilon subunit, at 5,126,781 to 5,127,395
_4300
Psyr_4301: Psyr_4301 - Protein of unknown function DUF88, at 5,127,414 to 5,127,890
_4301
Psyr_4302: Psyr_4302 - conserved hypothetical protein, at 5,128,171 to 5,128,875
_4302
Position (kb) 5126
5127
5128 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 5125.981 kb on + strand, within Psyr_4298 at 5125.981 kb on + strand, within Psyr_4298 at 5125.981 kb on + strand, within Psyr_4298 at 5125.981 kb on + strand, within Psyr_4298 at 5125.981 kb on + strand, within Psyr_4298 at 5125.981 kb on + strand, within Psyr_4298 at 5125.981 kb on + strand, within Psyr_4298 at 5125.982 kb on - strand, within Psyr_4298 at 5125.982 kb on - strand, within Psyr_4298 at 5125.982 kb on - strand, within Psyr_4298 at 5125.982 kb on - strand, within Psyr_4298 at 5125.982 kb on - strand, within Psyr_4298 at 5126.065 kb on + strand at 5126.065 kb on + strand at 5126.065 kb on + strand at 5126.065 kb on + strand at 5126.065 kb on + strand at 5126.066 kb on - strand at 5126.066 kb on - strand at 5126.066 kb on - strand at 5126.069 kb on + strand at 5126.070 kb on - strand at 5126.087 kb on - strand at 5126.087 kb on - strand at 5126.146 kb on - strand at 5126.164 kb on + strand at 5126.175 kb on + strand at 5126.175 kb on + strand at 5126.176 kb on - strand at 5126.176 kb on - strand at 5126.180 kb on + strand at 5126.181 kb on - strand at 5126.202 kb on + strand at 5126.203 kb on - strand at 5126.203 kb on - strand at 5126.253 kb on + strand at 5126.321 kb on - strand at 5126.321 kb on - strand at 5126.321 kb on - strand at 5126.321 kb on - strand at 5126.324 kb on + strand at 5126.324 kb on + strand at 5126.324 kb on + strand at 5126.324 kb on + strand at 5126.324 kb on + strand at 5126.324 kb on + strand at 5126.324 kb on + strand at 5126.324 kb on + strand at 5126.325 kb on - strand at 5126.325 kb on - strand at 5126.325 kb on - strand at 5126.325 kb on - strand at 5126.325 kb on - strand at 5126.325 kb on - strand at 5126.325 kb on - strand at 5126.325 kb on - strand at 5126.436 kb on + strand, within Psyr_4299 at 5126.437 kb on - strand, within Psyr_4299 at 5126.437 kb on - strand, within Psyr_4299 at 5126.463 kb on - strand, within Psyr_4299 at 5126.553 kb on - strand, within Psyr_4299 at 5126.553 kb on - strand, within Psyr_4299 at 5126.553 kb on - strand, within Psyr_4299 at 5126.553 kb on - strand, within Psyr_4299 at 5126.617 kb on - strand, within Psyr_4299 at 5126.744 kb on + strand at 5126.744 kb on + strand at 5126.744 kb on + strand at 5126.744 kb on + strand at 5126.745 kb on - strand at 5127.094 kb on + strand, within Psyr_4300 at 5127.094 kb on + strand, within Psyr_4300 at 5127.095 kb on - strand, within Psyr_4300 at 5127.248 kb on + strand, within Psyr_4300 at 5127.248 kb on + strand, within Psyr_4300 at 5127.339 kb on - strand at 5127.383 kb on + strand at 5127.384 kb on - strand at 5127.384 kb on - strand at 5127.384 kb on - strand at 5127.384 kb on - strand at 5127.384 kb on - strand at 5127.384 kb on - strand at 5127.411 kb on - strand at 5127.411 kb on - strand at 5127.500 kb on - strand, within Psyr_4301 at 5127.669 kb on - strand, within Psyr_4301 at 5127.670 kb on + strand, within Psyr_4301 at 5127.671 kb on - strand, within Psyr_4301 at 5127.671 kb on - strand, within Psyr_4301 at 5127.671 kb on - strand, within Psyr_4301 at 5127.671 kb on - strand, within Psyr_4301 at 5127.671 kb on - strand, within Psyr_4301 at 5127.671 kb on - strand, within Psyr_4301 at 5127.671 kb on - strand, within Psyr_4301 at 5127.671 kb on - strand, within Psyr_4301 at 5127.671 kb on - strand, within Psyr_4301 at 5127.671 kb on - strand, within Psyr_4301 at 5127.709 kb on - strand, within Psyr_4301 at 5127.811 kb on + strand, within Psyr_4301 at 5127.847 kb on + strand at 5127.847 kb on + strand at 5127.847 kb on + strand at 5127.847 kb on + strand at 5127.847 kb on + strand at 5127.847 kb on + strand at 5127.847 kb on + strand at 5127.848 kb on - strand at 5127.848 kb on - strand at 5127.848 kb on - strand at 5127.848 kb on - strand at 5127.848 kb on - strand at 5127.850 kb on + strand at 5127.850 kb on + strand at 5127.850 kb on + strand at 5127.850 kb on + strand at 5127.851 kb on - strand at 5127.851 kb on - strand at 5127.958 kb on + strand at 5127.958 kb on + strand at 5128.235 kb on + strand at 5128.235 kb on + strand at 5128.236 kb on - strand at 5128.236 kb on - strand at 5128.362 kb on + strand, within Psyr_4302 at 5128.363 kb on - strand, within Psyr_4302
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 5,125,981 + Psyr_4298 0.82 +0.1 5,125,981 + Psyr_4298 0.82 +0.9 5,125,981 + Psyr_4298 0.82 -0.3 5,125,981 + Psyr_4298 0.82 -0.7 5,125,981 + Psyr_4298 0.82 -0.8 5,125,981 + Psyr_4298 0.82 -2.2 5,125,981 + Psyr_4298 0.82 +0.4 5,125,982 - Psyr_4298 0.83 +1.1 5,125,982 - Psyr_4298 0.83 -1.6 5,125,982 - Psyr_4298 0.83 +0.6 5,125,982 - Psyr_4298 0.83 -1.1 5,125,982 - Psyr_4298 0.83 -1.7 5,126,065 + -0.5 5,126,065 + +0.2 5,126,065 + +0.3 5,126,065 + -0.1 5,126,065 + +0.4 5,126,066 - -1.1 5,126,066 - -0.7 5,126,066 - -0.5 5,126,069 + +3.2 5,126,070 - +0.4 5,126,087 - +0.2 5,126,087 - -0.8 5,126,146 - -1.1 5,126,164 + +0.1 5,126,175 + +0.2 5,126,175 + -0.5 5,126,176 - -0.3 5,126,176 - +0.4 5,126,180 + -3.2 5,126,181 - +0.4 5,126,202 + +2.8 5,126,203 - +0.0 5,126,203 - +0.2 5,126,253 + +0.2 5,126,321 - +0.5 5,126,321 - +1.6 5,126,321 - -1.4 5,126,321 - +0.2 5,126,324 + +0.3 5,126,324 + +0.2 5,126,324 + +0.5 5,126,324 + -0.3 5,126,324 + +0.7 5,126,324 + +1.8 5,126,324 + +0.3 5,126,324 + +0.7 5,126,325 - -0.4 5,126,325 - -0.8 5,126,325 - -0.6 5,126,325 - -0.4 5,126,325 - -0.5 5,126,325 - +3.2 5,126,325 - +0.8 5,126,325 - +0.7 5,126,436 + Psyr_4299 0.27 -0.1 5,126,437 - Psyr_4299 0.27 +0.5 5,126,437 - Psyr_4299 0.27 -0.8 5,126,463 - Psyr_4299 0.33 +1.0 5,126,553 - Psyr_4299 0.52 -1.4 5,126,553 - Psyr_4299 0.52 -1.2 5,126,553 - Psyr_4299 0.52 -1.8 5,126,553 - Psyr_4299 0.52 -0.8 5,126,617 - Psyr_4299 0.66 +0.9 5,126,744 + -0.8 5,126,744 + -0.8 5,126,744 + -0.4 5,126,744 + +1.6 5,126,745 - +0.8 5,127,094 + Psyr_4300 0.51 -2.4 5,127,094 + Psyr_4300 0.51 -0.8 5,127,095 - Psyr_4300 0.51 +0.4 5,127,248 + Psyr_4300 0.76 -1.3 5,127,248 + Psyr_4300 0.76 -1.1 5,127,339 - -0.6 5,127,383 + -1.4 5,127,384 - +2.8 5,127,384 - +1.1 5,127,384 - -0.9 5,127,384 - -0.6 5,127,384 - -0.4 5,127,384 - -0.1 5,127,411 - -0.1 5,127,411 - -0.6 5,127,500 - Psyr_4301 0.18 +1.3 5,127,669 - Psyr_4301 0.53 +1.6 5,127,670 + Psyr_4301 0.54 +0.8 5,127,671 - Psyr_4301 0.54 +1.5 5,127,671 - Psyr_4301 0.54 +1.8 5,127,671 - Psyr_4301 0.54 +0.2 5,127,671 - Psyr_4301 0.54 +0.5 5,127,671 - Psyr_4301 0.54 -1.5 5,127,671 - Psyr_4301 0.54 +0.4 5,127,671 - Psyr_4301 0.54 +1.1 5,127,671 - Psyr_4301 0.54 +0.6 5,127,671 - Psyr_4301 0.54 +0.1 5,127,671 - Psyr_4301 0.54 -0.4 5,127,709 - Psyr_4301 0.62 -1.1 5,127,811 + Psyr_4301 0.83 +3.4 5,127,847 + +3.8 5,127,847 + -2.4 5,127,847 + -0.1 5,127,847 + +0.2 5,127,847 + -1.7 5,127,847 + +0.5 5,127,847 + +0.6 5,127,848 - -2.8 5,127,848 - -1.4 5,127,848 - +0.5 5,127,848 - -0.6 5,127,848 - -1.3 5,127,850 + -0.5 5,127,850 + -1.4 5,127,850 + -0.0 5,127,850 + -0.8 5,127,851 - +2.1 5,127,851 - +0.3 5,127,958 + +0.1 5,127,958 + +0.6 5,128,235 + -0.7 5,128,235 + +1.8 5,128,236 - +0.5 5,128,236 - +0.2 5,128,362 + Psyr_4302 0.27 +2.2 5,128,363 - Psyr_4302 0.27 +0.6
Or see this region's nucleotide sequence