Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4176 and Psyr_4177 are separated by 175 nucleotides Psyr_4177 and Psyr_4178 are separated by 160 nucleotides Psyr_4178 and Psyr_4179 are separated by 3 nucleotides Psyr_4179 and Psyr_4180 are separated by 159 nucleotides
Psyr_4176: Psyr_4176 - Polyribonucleotide nucleotidyltransferase, at 4,967,358 to 4,969,463
_4176
Psyr_4177: Psyr_4177 - SSU ribosomal protein S15P, at 4,969,639 to 4,969,908
_4177
Psyr_4178: Psyr_4178 - tRNA pseudouridine synthase B, at 4,970,069 to 4,970,986
_4178
Psyr_4179: Psyr_4179 - ribosome-binding factor A, at 4,970,990 to 4,971,406
_4179
Psyr_4180: Psyr_4180 - bacterial translation initiation factor 2 (bIF-2), at 4,971,566 to 4,974,091
_4180
Position (kb) 4970
4971 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4970.218 kb on - strand, within Psyr_4178 at 4970.401 kb on - strand, within Psyr_4178 at 4970.401 kb on - strand, within Psyr_4178 at 4970.401 kb on - strand, within Psyr_4178 at 4970.401 kb on - strand, within Psyr_4178 at 4970.401 kb on - strand, within Psyr_4178 at 4970.401 kb on - strand, within Psyr_4178 at 4970.401 kb on - strand, within Psyr_4178 at 4970.452 kb on - strand, within Psyr_4178 at 4970.452 kb on - strand, within Psyr_4178 at 4970.452 kb on - strand, within Psyr_4178 at 4970.508 kb on - strand, within Psyr_4178 at 4970.508 kb on - strand, within Psyr_4178 at 4970.525 kb on - strand, within Psyr_4178 at 4970.525 kb on - strand, within Psyr_4178 at 4970.525 kb on - strand, within Psyr_4178 at 4970.525 kb on - strand, within Psyr_4178 at 4970.553 kb on - strand, within Psyr_4178 at 4970.577 kb on + strand, within Psyr_4178 at 4970.578 kb on - strand, within Psyr_4178 at 4970.578 kb on - strand, within Psyr_4178 at 4970.578 kb on - strand, within Psyr_4178 at 4970.610 kb on + strand, within Psyr_4178 at 4970.610 kb on + strand, within Psyr_4178 at 4970.610 kb on + strand, within Psyr_4178 at 4970.611 kb on - strand, within Psyr_4178 at 4970.611 kb on - strand, within Psyr_4178 at 4970.622 kb on - strand, within Psyr_4178 at 4970.760 kb on + strand, within Psyr_4178 at 4970.761 kb on - strand, within Psyr_4178 at 4970.761 kb on - strand, within Psyr_4178 at 4970.784 kb on + strand, within Psyr_4178 at 4970.785 kb on - strand, within Psyr_4178 at 4970.966 kb on + strand at 4970.966 kb on + strand at 4970.966 kb on + strand at 4970.968 kb on + strand at 4970.968 kb on + strand at 4970.968 kb on + strand at 4970.968 kb on + strand at 4970.968 kb on + strand at 4970.969 kb on - strand at 4970.969 kb on - strand at 4970.969 kb on - strand at 4970.969 kb on - strand at 4970.969 kb on - strand at 4970.992 kb on - strand at 4970.992 kb on - strand at 4971.001 kb on + strand at 4971.002 kb on - strand at 4971.114 kb on + strand, within Psyr_4179 at 4971.394 kb on - strand at 4971.394 kb on - strand at 4971.414 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 4,970,218 - Psyr_4178 0.16 -0.9 4,970,401 - Psyr_4178 0.36 +0.6 4,970,401 - Psyr_4178 0.36 +0.0 4,970,401 - Psyr_4178 0.36 +1.7 4,970,401 - Psyr_4178 0.36 -0.9 4,970,401 - Psyr_4178 0.36 -0.1 4,970,401 - Psyr_4178 0.36 +0.0 4,970,401 - Psyr_4178 0.36 -0.4 4,970,452 - Psyr_4178 0.42 -1.1 4,970,452 - Psyr_4178 0.42 +0.0 4,970,452 - Psyr_4178 0.42 -0.8 4,970,508 - Psyr_4178 0.48 +0.0 4,970,508 - Psyr_4178 0.48 -1.4 4,970,525 - Psyr_4178 0.50 -2.3 4,970,525 - Psyr_4178 0.50 -0.6 4,970,525 - Psyr_4178 0.50 -1.9 4,970,525 - Psyr_4178 0.50 -2.7 4,970,553 - Psyr_4178 0.53 +0.0 4,970,577 + Psyr_4178 0.55 -2.8 4,970,578 - Psyr_4178 0.55 -1.2 4,970,578 - Psyr_4178 0.55 -0.3 4,970,578 - Psyr_4178 0.55 -1.3 4,970,610 + Psyr_4178 0.59 +0.9 4,970,610 + Psyr_4178 0.59 -2.0 4,970,610 + Psyr_4178 0.59 +3.2 4,970,611 - Psyr_4178 0.59 -1.1 4,970,611 - Psyr_4178 0.59 -3.0 4,970,622 - Psyr_4178 0.60 -1.1 4,970,760 + Psyr_4178 0.75 +0.6 4,970,761 - Psyr_4178 0.75 -0.4 4,970,761 - Psyr_4178 0.75 -0.8 4,970,784 + Psyr_4178 0.78 +0.2 4,970,785 - Psyr_4178 0.78 -0.8 4,970,966 + -0.7 4,970,966 + -1.0 4,970,966 + +0.7 4,970,968 + +0.0 4,970,968 + -1.6 4,970,968 + -1.3 4,970,968 + +0.7 4,970,968 + -0.2 4,970,969 - +0.1 4,970,969 - -0.1 4,970,969 - -2.3 4,970,969 - -3.2 4,970,969 - +2.2 4,970,992 - -0.2 4,970,992 - +0.4 4,971,001 + -0.7 4,971,002 - +0.8 4,971,114 + Psyr_4179 0.30 +0.6 4,971,394 - -0.8 4,971,394 - -2.3 4,971,414 - -1.8
Or see this region's nucleotide sequence