Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3932 and Psyr_3933 overlap by 4 nucleotides Psyr_3933 and Psyr_3934 are separated by 22 nucleotides Psyr_3934 and Psyr_3935 are separated by 124 nucleotides Psyr_3935 and Psyr_3936 are separated by 10 nucleotides
Psyr_3932: Psyr_3932 - Cobalamin synthesis protein/P47K:Cobalamin synthesis protein/P47K, at 4,678,015 to 4,679,022
_3932
Psyr_3933: Psyr_3933 - conserved hypothetical protein, at 4,679,019 to 4,679,318
_3933
Psyr_3934: Psyr_3934 - Short-chain dehydrogenase/reductase SDR, at 4,679,341 to 4,680,090
_3934
Psyr_3935: Psyr_3935 - Short-chain dehydrogenase/reductase SDR, at 4,680,215 to 4,680,976
_3935
Psyr_3936: Psyr_3936 - General substrate transporter:Major facilitator superfamily, at 4,680,987 to 4,682,327
_3936
Position (kb)
4679
4680
4681 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4678.464 kb on + strand, within Psyr_3932 at 4679.158 kb on + strand, within Psyr_3933 at 4679.158 kb on + strand, within Psyr_3933 at 4679.159 kb on - strand, within Psyr_3933 at 4679.226 kb on - strand, within Psyr_3933 at 4679.226 kb on - strand, within Psyr_3933 at 4679.776 kb on - strand, within Psyr_3934 at 4680.015 kb on - strand, within Psyr_3934 at 4680.520 kb on - strand, within Psyr_3935 at 4680.520 kb on - strand, within Psyr_3935 at 4680.543 kb on + strand, within Psyr_3935 at 4680.543 kb on + strand, within Psyr_3935 at 4680.543 kb on + strand, within Psyr_3935 at 4680.543 kb on + strand, within Psyr_3935 at 4680.544 kb on - strand, within Psyr_3935 at 4680.544 kb on - strand, within Psyr_3935 at 4680.873 kb on + strand, within Psyr_3935 at 4680.968 kb on - strand at 4681.066 kb on + strand at 4681.067 kb on - strand at 4681.067 kb on - strand at 4681.067 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 4,678,464 + Psyr_3932 0.45 +1.1 4,679,158 + Psyr_3933 0.46 -1.4 4,679,158 + Psyr_3933 0.46 -1.5 4,679,159 - Psyr_3933 0.47 +0.1 4,679,226 - Psyr_3933 0.69 +2.1 4,679,226 - Psyr_3933 0.69 -1.7 4,679,776 - Psyr_3934 0.58 -1.2 4,680,015 - Psyr_3934 0.90 -0.7 4,680,520 - Psyr_3935 0.40 -0.2 4,680,520 - Psyr_3935 0.40 +2.7 4,680,543 + Psyr_3935 0.43 -1.2 4,680,543 + Psyr_3935 0.43 +0.1 4,680,543 + Psyr_3935 0.43 +0.2 4,680,543 + Psyr_3935 0.43 -1.2 4,680,544 - Psyr_3935 0.43 +1.8 4,680,544 - Psyr_3935 0.43 -0.9 4,680,873 + Psyr_3935 0.86 +0.9 4,680,968 - +0.7 4,681,066 + -2.5 4,681,067 - +0.2 4,681,067 - -1.2 4,681,067 - -2.3
Or see this region's nucleotide sequence