Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3651 and Psyr_3652 are separated by 12 nucleotides Psyr_3652 and Psyr_3653 are separated by 207 nucleotides Psyr_3653 and Psyr_3654 are separated by 27 nucleotides Psyr_3654 and Psyr_3655 are separated by 31 nucleotides
Psyr_3651: Psyr_3651 - 2-phosphoglycolate phosphatase, prokaryotic:HAD-superfamily hydrolase, subfamily IA, variant 3:HAD-superfamily hydrolase, subfamily IA, variant 1, at 4,336,935 to 4,337,606
_3651
Psyr_3652: Psyr_3652 - Short-chain dehydrogenase/reductase SDR, at 4,337,619 to 4,338,359
_3652
Psyr_3653: Psyr_3653 - GGDEF domain protein, at 4,338,567 to 4,339,493
_3653
Psyr_3654: Psyr_3654 - conserved hypothetical protein, at 4,339,521 to 4,340,303
_3654
Psyr_3655: Psyr_3655 - diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s), at 4,340,335 to 4,342,797
_3655
Position (kb) 4338
4339
4340 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 4337.616 kb on + strand at 4337.616 kb on + strand at 4337.616 kb on + strand at 4337.616 kb on + strand at 4337.616 kb on + strand at 4337.616 kb on + strand at 4337.617 kb on - strand at 4337.617 kb on - strand at 4337.617 kb on - strand at 4337.617 kb on - strand at 4337.617 kb on - strand at 4337.617 kb on - strand at 4337.715 kb on + strand, within Psyr_3652 at 4337.821 kb on + strand, within Psyr_3652 at 4337.857 kb on - strand, within Psyr_3652 at 4337.899 kb on + strand, within Psyr_3652 at 4337.916 kb on - strand, within Psyr_3652 at 4337.939 kb on + strand, within Psyr_3652 at 4338.082 kb on + strand, within Psyr_3652 at 4338.082 kb on + strand, within Psyr_3652 at 4338.083 kb on - strand, within Psyr_3652 at 4338.083 kb on - strand, within Psyr_3652 at 4338.083 kb on - strand, within Psyr_3652 at 4338.083 kb on - strand, within Psyr_3652 at 4338.083 kb on - strand, within Psyr_3652 at 4338.083 kb on - strand, within Psyr_3652 at 4338.298 kb on - strand at 4338.417 kb on - strand at 4338.468 kb on - strand at 4338.481 kb on + strand at 4338.546 kb on + strand at 4338.546 kb on + strand at 4338.546 kb on + strand at 4338.546 kb on + strand at 4338.547 kb on - strand at 4338.547 kb on - strand at 4338.547 kb on - strand at 4338.547 kb on - strand at 4338.632 kb on + strand at 4338.684 kb on + strand, within Psyr_3653 at 4338.754 kb on + strand, within Psyr_3653 at 4338.754 kb on + strand, within Psyr_3653 at 4338.754 kb on + strand, within Psyr_3653 at 4338.755 kb on - strand, within Psyr_3653 at 4338.755 kb on - strand, within Psyr_3653 at 4338.755 kb on - strand, within Psyr_3653 at 4338.755 kb on - strand, within Psyr_3653 at 4338.755 kb on - strand, within Psyr_3653 at 4338.755 kb on - strand, within Psyr_3653 at 4338.755 kb on - strand, within Psyr_3653 at 4338.786 kb on + strand, within Psyr_3653 at 4338.786 kb on + strand, within Psyr_3653 at 4338.786 kb on + strand, within Psyr_3653 at 4338.787 kb on - strand, within Psyr_3653 at 4338.960 kb on + strand, within Psyr_3653 at 4339.152 kb on + strand, within Psyr_3653 at 4339.152 kb on + strand, within Psyr_3653 at 4339.152 kb on + strand, within Psyr_3653 at 4339.446 kb on + strand at 4339.446 kb on + strand at 4339.446 kb on + strand at 4339.446 kb on + strand at 4339.446 kb on + strand at 4339.447 kb on - strand at 4339.594 kb on + strand at 4339.594 kb on + strand at 4339.594 kb on + strand at 4339.594 kb on + strand at 4339.594 kb on + strand at 4339.594 kb on + strand at 4339.594 kb on + strand at 4339.594 kb on + strand at 4339.594 kb on + strand at 4339.594 kb on + strand at 4339.594 kb on + strand at 4339.595 kb on - strand at 4339.595 kb on - strand at 4339.595 kb on - strand at 4339.595 kb on - strand at 4339.595 kb on - strand at 4339.595 kb on - strand at 4339.600 kb on + strand, within Psyr_3654 at 4339.600 kb on + strand, within Psyr_3654 at 4339.600 kb on + strand, within Psyr_3654 at 4339.600 kb on + strand, within Psyr_3654 at 4339.600 kb on + strand, within Psyr_3654 at 4339.600 kb on + strand at 4339.601 kb on - strand, within Psyr_3654 at 4339.601 kb on - strand, within Psyr_3654 at 4339.601 kb on - strand, within Psyr_3654 at 4339.601 kb on - strand, within Psyr_3654 at 4339.601 kb on - strand, within Psyr_3654 at 4339.601 kb on - strand, within Psyr_3654 at 4339.601 kb on - strand, within Psyr_3654 at 4339.601 kb on - strand, within Psyr_3654 at 4339.601 kb on - strand, within Psyr_3654 at 4339.601 kb on - strand, within Psyr_3654 at 4339.607 kb on - strand, within Psyr_3654 at 4339.674 kb on - strand, within Psyr_3654 at 4339.674 kb on - strand, within Psyr_3654 at 4339.674 kb on - strand, within Psyr_3654 at 4339.674 kb on - strand, within Psyr_3654 at 4339.837 kb on + strand, within Psyr_3654 at 4339.837 kb on + strand, within Psyr_3654 at 4339.837 kb on + strand, within Psyr_3654 at 4339.838 kb on - strand, within Psyr_3654 at 4339.838 kb on - strand, within Psyr_3654 at 4339.838 kb on - strand, within Psyr_3654 at 4339.838 kb on - strand, within Psyr_3654 at 4339.838 kb on - strand, within Psyr_3654 at 4339.838 kb on - strand, within Psyr_3654 at 4339.838 kb on - strand, within Psyr_3654 at 4339.838 kb on - strand, within Psyr_3654 at 4339.838 kb on - strand, within Psyr_3654 at 4339.838 kb on - strand, within Psyr_3654 at 4339.838 kb on - strand, within Psyr_3654 at 4339.838 kb on - strand, within Psyr_3654 at 4339.985 kb on - strand at 4340.092 kb on + strand, within Psyr_3654 at 4340.092 kb on + strand, within Psyr_3654 at 4340.092 kb on + strand, within Psyr_3654 at 4340.092 kb on + strand, within Psyr_3654 at 4340.092 kb on + strand, within Psyr_3654 at 4340.092 kb on + strand, within Psyr_3654 at 4340.092 kb on + strand, within Psyr_3654 at 4340.092 kb on + strand at 4340.093 kb on - strand, within Psyr_3654 at 4340.093 kb on - strand, within Psyr_3654 at 4340.093 kb on - strand, within Psyr_3654 at 4340.093 kb on - strand, within Psyr_3654 at 4340.093 kb on - strand, within Psyr_3654 at 4340.093 kb on - strand, within Psyr_3654 at 4340.093 kb on - strand, within Psyr_3654 at 4340.093 kb on - strand, within Psyr_3654 at 4340.093 kb on - strand, within Psyr_3654 at 4340.093 kb on - strand, within Psyr_3654 at 4340.135 kb on - strand, within Psyr_3654 at 4340.183 kb on - strand, within Psyr_3654 at 4340.317 kb on + strand at 4340.341 kb on + strand at 4340.341 kb on + strand at 4340.380 kb on + strand at 4340.391 kb on + strand at 4340.392 kb on - strand at 4340.393 kb on + strand at 4340.393 kb on + strand at 4340.394 kb on - strand at 4340.394 kb on - strand at 4340.394 kb on - strand at 4340.436 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 4,337,616 + +0.7 4,337,616 + +1.2 4,337,616 + +0.8 4,337,616 + -1.5 4,337,616 + +0.6 4,337,616 + +0.3 4,337,617 - +1.1 4,337,617 - -2.1 4,337,617 - -0.3 4,337,617 - -1.1 4,337,617 - +0.1 4,337,617 - -0.9 4,337,715 + Psyr_3652 0.13 -0.8 4,337,821 + Psyr_3652 0.27 -0.0 4,337,857 - Psyr_3652 0.32 +0.6 4,337,899 + Psyr_3652 0.38 -0.7 4,337,916 - Psyr_3652 0.40 -2.1 4,337,939 + Psyr_3652 0.43 -0.7 4,338,082 + Psyr_3652 0.62 -0.4 4,338,082 + Psyr_3652 0.62 -1.0 4,338,083 - Psyr_3652 0.63 -0.6 4,338,083 - Psyr_3652 0.63 -0.0 4,338,083 - Psyr_3652 0.63 -0.5 4,338,083 - Psyr_3652 0.63 +0.7 4,338,083 - Psyr_3652 0.63 +2.1 4,338,083 - Psyr_3652 0.63 +0.3 4,338,298 - +0.2 4,338,417 - -0.1 4,338,468 - +1.5 4,338,481 + +0.6 4,338,546 + -0.0 4,338,546 + -3.8 4,338,546 + +1.9 4,338,546 + +0.3 4,338,547 - +0.2 4,338,547 - -0.5 4,338,547 - -0.3 4,338,547 - -0.3 4,338,632 + +0.1 4,338,684 + Psyr_3653 0.13 +2.7 4,338,754 + Psyr_3653 0.20 +1.9 4,338,754 + Psyr_3653 0.20 +0.1 4,338,754 + Psyr_3653 0.20 -0.4 4,338,755 - Psyr_3653 0.20 -0.2 4,338,755 - Psyr_3653 0.20 +1.7 4,338,755 - Psyr_3653 0.20 +1.1 4,338,755 - Psyr_3653 0.20 +0.7 4,338,755 - Psyr_3653 0.20 -0.3 4,338,755 - Psyr_3653 0.20 +0.4 4,338,755 - Psyr_3653 0.20 +1.1 4,338,786 + Psyr_3653 0.24 -0.9 4,338,786 + Psyr_3653 0.24 +0.3 4,338,786 + Psyr_3653 0.24 +0.3 4,338,787 - Psyr_3653 0.24 +2.8 4,338,960 + Psyr_3653 0.42 +2.1 4,339,152 + Psyr_3653 0.63 +0.8 4,339,152 + Psyr_3653 0.63 -0.3 4,339,152 + Psyr_3653 0.63 -0.3 4,339,446 + +1.1 4,339,446 + +1.5 4,339,446 + +0.7 4,339,446 + -0.4 4,339,446 + -0.9 4,339,447 - +1.1 4,339,594 + +1.7 4,339,594 + -0.3 4,339,594 + +0.3 4,339,594 + +1.7 4,339,594 + +0.6 4,339,594 + +1.3 4,339,594 + -0.5 4,339,594 + +0.4 4,339,594 + -0.3 4,339,594 + -1.0 4,339,594 + -1.2 4,339,595 - +0.5 4,339,595 - +1.8 4,339,595 - -1.2 4,339,595 - +0.3 4,339,595 - +1.1 4,339,595 - +1.8 4,339,600 + Psyr_3654 0.10 +0.8 4,339,600 + Psyr_3654 0.10 -0.5 4,339,600 + Psyr_3654 0.10 -0.4 4,339,600 + Psyr_3654 0.10 +0.1 4,339,600 + Psyr_3654 0.10 +0.1 4,339,600 + +2.7 4,339,601 - Psyr_3654 0.10 +0.0 4,339,601 - Psyr_3654 0.10 -0.9 4,339,601 - Psyr_3654 0.10 -1.4 4,339,601 - Psyr_3654 0.10 -0.6 4,339,601 - Psyr_3654 0.10 +0.3 4,339,601 - Psyr_3654 0.10 +0.2 4,339,601 - Psyr_3654 0.10 +0.3 4,339,601 - Psyr_3654 0.10 +0.1 4,339,601 - Psyr_3654 0.10 -0.9 4,339,601 - Psyr_3654 0.10 +0.1 4,339,607 - Psyr_3654 0.11 -1.4 4,339,674 - Psyr_3654 0.20 -0.2 4,339,674 - Psyr_3654 0.20 -0.2 4,339,674 - Psyr_3654 0.20 -0.0 4,339,674 - Psyr_3654 0.20 -2.1 4,339,837 + Psyr_3654 0.40 +1.0 4,339,837 + Psyr_3654 0.40 +3.4 4,339,837 + Psyr_3654 0.40 +0.3 4,339,838 - Psyr_3654 0.40 +1.5 4,339,838 - Psyr_3654 0.40 -0.5 4,339,838 - Psyr_3654 0.40 -3.5 4,339,838 - Psyr_3654 0.40 +0.2 4,339,838 - Psyr_3654 0.40 -0.2 4,339,838 - Psyr_3654 0.40 -1.9 4,339,838 - Psyr_3654 0.40 +1.9 4,339,838 - Psyr_3654 0.40 +0.4 4,339,838 - Psyr_3654 0.40 +0.7 4,339,838 - Psyr_3654 0.40 +1.1 4,339,838 - Psyr_3654 0.40 +0.6 4,339,838 - Psyr_3654 0.40 +0.1 4,339,985 - +1.1 4,340,092 + Psyr_3654 0.73 +0.3 4,340,092 + Psyr_3654 0.73 +0.3 4,340,092 + Psyr_3654 0.73 -1.3 4,340,092 + Psyr_3654 0.73 +0.6 4,340,092 + Psyr_3654 0.73 -0.9 4,340,092 + Psyr_3654 0.73 +0.4 4,340,092 + Psyr_3654 0.73 -1.2 4,340,092 + +1.1 4,340,093 - Psyr_3654 0.73 -2.2 4,340,093 - Psyr_3654 0.73 -0.6 4,340,093 - Psyr_3654 0.73 -0.0 4,340,093 - Psyr_3654 0.73 -1.0 4,340,093 - Psyr_3654 0.73 -0.5 4,340,093 - Psyr_3654 0.73 +0.3 4,340,093 - Psyr_3654 0.73 +1.4 4,340,093 - Psyr_3654 0.73 +1.4 4,340,093 - Psyr_3654 0.73 -0.5 4,340,093 - Psyr_3654 0.73 -0.1 4,340,135 - Psyr_3654 0.78 -0.5 4,340,183 - Psyr_3654 0.85 +0.7 4,340,317 + -0.3 4,340,341 + -1.8 4,340,341 + +0.6 4,340,380 + +1.3 4,340,391 + -1.6 4,340,392 - -0.1 4,340,393 + -0.3 4,340,393 + +0.1 4,340,394 - +0.1 4,340,394 - -0.1 4,340,394 - +2.9 4,340,436 - -1.3
Or see this region's nucleotide sequence