Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3561 and Psyr_3562 are separated by 48 nucleotides Psyr_3562 and Psyr_3563 overlap by 1 nucleotides
Psyr_3561: Psyr_3561 - succinylarginine dihydrolase, at 4,241,366 to 4,242,712
_3561
Psyr_3562: Psyr_3562 - succinylglutamic semialdehyde dehydrogenase, at 4,242,761 to 4,244,227
_3562
Psyr_3563: Psyr_3563 - arginine succinyltransferase, at 4,244,227 to 4,245,252
_3563
Position (kb)
4242
4243
4244
4245 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4241.787 kb on + strand, within Psyr_3561 at 4241.787 kb on + strand, within Psyr_3561 at 4241.922 kb on + strand, within Psyr_3561 at 4241.922 kb on + strand, within Psyr_3561 at 4241.969 kb on + strand, within Psyr_3561 at 4241.969 kb on + strand, within Psyr_3561 at 4242.006 kb on + strand, within Psyr_3561 at 4242.007 kb on - strand, within Psyr_3561 at 4242.007 kb on - strand, within Psyr_3561 at 4242.306 kb on + strand, within Psyr_3561 at 4242.306 kb on + strand, within Psyr_3561 at 4242.306 kb on + strand, within Psyr_3561 at 4242.310 kb on + strand, within Psyr_3561 at 4242.310 kb on + strand, within Psyr_3561 at 4242.310 kb on + strand, within Psyr_3561 at 4242.310 kb on + strand, within Psyr_3561 at 4242.311 kb on - strand, within Psyr_3561 at 4242.311 kb on - strand, within Psyr_3561 at 4242.311 kb on - strand, within Psyr_3561 at 4242.311 kb on - strand, within Psyr_3561 at 4242.311 kb on - strand, within Psyr_3561 at 4242.645 kb on + strand at 4242.661 kb on - strand at 4242.739 kb on - strand at 4242.740 kb on + strand at 4242.740 kb on + strand at 4242.741 kb on - strand at 4242.858 kb on + strand at 4243.437 kb on + strand, within Psyr_3562 at 4243.437 kb on + strand, within Psyr_3562 at 4243.437 kb on + strand, within Psyr_3562 at 4243.437 kb on + strand, within Psyr_3562 at 4243.437 kb on + strand, within Psyr_3562 at 4243.437 kb on + strand, within Psyr_3562 at 4243.438 kb on - strand, within Psyr_3562 at 4243.438 kb on - strand, within Psyr_3562 at 4243.438 kb on - strand, within Psyr_3562 at 4243.438 kb on - strand, within Psyr_3562 at 4244.214 kb on + strand at 4244.214 kb on + strand at 4244.214 kb on + strand at 4244.214 kb on + strand at 4244.214 kb on + strand at 4244.214 kb on + strand at 4244.214 kb on + strand at 4244.215 kb on - strand at 4244.215 kb on - strand at 4244.215 kb on - strand at 4244.215 kb on - strand at 4244.215 kb on - strand at 4244.215 kb on - strand at 4244.215 kb on - strand at 4244.215 kb on - strand at 4244.222 kb on - strand at 4244.384 kb on + strand, within Psyr_3563 at 4244.384 kb on + strand, within Psyr_3563 at 4244.385 kb on - strand, within Psyr_3563 at 4244.385 kb on - strand, within Psyr_3563 at 4244.385 kb on - strand, within Psyr_3563 at 4244.513 kb on + strand, within Psyr_3563 at 4244.621 kb on + strand, within Psyr_3563 at 4244.622 kb on - strand, within Psyr_3563 at 4244.622 kb on - strand, within Psyr_3563 at 4244.948 kb on + strand, within Psyr_3563 at 4244.949 kb on - strand, within Psyr_3563 at 4244.999 kb on + strand, within Psyr_3563 at 4245.000 kb on - strand, within Psyr_3563 at 4245.084 kb on - strand, within Psyr_3563 at 4245.084 kb on - strand, within Psyr_3563 at 4245.137 kb on - strand, within Psyr_3563
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 4,241,787 + Psyr_3561 0.31 +0.6 4,241,787 + Psyr_3561 0.31 +0.7 4,241,922 + Psyr_3561 0.41 +3.1 4,241,922 + Psyr_3561 0.41 -1.2 4,241,969 + Psyr_3561 0.45 +0.5 4,241,969 + Psyr_3561 0.45 +1.1 4,242,006 + Psyr_3561 0.48 +0.4 4,242,007 - Psyr_3561 0.48 -0.5 4,242,007 - Psyr_3561 0.48 -1.1 4,242,306 + Psyr_3561 0.70 +0.4 4,242,306 + Psyr_3561 0.70 -0.0 4,242,306 + Psyr_3561 0.70 -0.2 4,242,310 + Psyr_3561 0.70 +0.7 4,242,310 + Psyr_3561 0.70 -0.7 4,242,310 + Psyr_3561 0.70 +3.4 4,242,310 + Psyr_3561 0.70 -1.3 4,242,311 - Psyr_3561 0.70 +0.4 4,242,311 - Psyr_3561 0.70 +1.7 4,242,311 - Psyr_3561 0.70 -0.2 4,242,311 - Psyr_3561 0.70 +0.4 4,242,311 - Psyr_3561 0.70 +1.3 4,242,645 + +0.0 4,242,661 - +1.4 4,242,739 - -0.2 4,242,740 + -0.4 4,242,740 + -0.6 4,242,741 - +0.1 4,242,858 + -0.5 4,243,437 + Psyr_3562 0.46 +0.7 4,243,437 + Psyr_3562 0.46 +0.2 4,243,437 + Psyr_3562 0.46 +2.1 4,243,437 + Psyr_3562 0.46 +0.4 4,243,437 + Psyr_3562 0.46 -2.1 4,243,437 + Psyr_3562 0.46 -0.1 4,243,438 - Psyr_3562 0.46 -2.3 4,243,438 - Psyr_3562 0.46 +0.4 4,243,438 - Psyr_3562 0.46 +1.8 4,243,438 - Psyr_3562 0.46 +1.3 4,244,214 + -0.2 4,244,214 + +2.7 4,244,214 + -0.3 4,244,214 + +1.1 4,244,214 + -0.5 4,244,214 + -0.1 4,244,214 + +0.9 4,244,215 - +0.5 4,244,215 - -0.1 4,244,215 - -1.7 4,244,215 - +1.0 4,244,215 - +0.0 4,244,215 - -0.6 4,244,215 - +0.1 4,244,215 - +0.8 4,244,222 - -1.0 4,244,384 + Psyr_3563 0.15 +0.8 4,244,384 + Psyr_3563 0.15 -1.3 4,244,385 - Psyr_3563 0.15 -0.4 4,244,385 - Psyr_3563 0.15 +0.2 4,244,385 - Psyr_3563 0.15 -1.1 4,244,513 + Psyr_3563 0.28 +0.9 4,244,621 + Psyr_3563 0.38 -0.6 4,244,622 - Psyr_3563 0.38 +0.1 4,244,622 - Psyr_3563 0.38 +0.5 4,244,948 + Psyr_3563 0.70 +0.3 4,244,949 - Psyr_3563 0.70 +0.7 4,244,999 + Psyr_3563 0.75 -1.3 4,245,000 - Psyr_3563 0.75 +0.7 4,245,084 - Psyr_3563 0.84 -0.9 4,245,084 - Psyr_3563 0.84 -0.1 4,245,137 - Psyr_3563 0.89 -0.9
Or see this region's nucleotide sequence