Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3474 and Psyr_3475 are separated by 84 nucleotides Psyr_3475 and Psyr_3476 are separated by 46 nucleotides Psyr_3476 and Psyr_3477 are separated by 475 nucleotides
Psyr_3474: Psyr_3474 - Flagellar L-ring protein, at 4,144,839 to 4,145,552
_3474
Psyr_3475: Psyr_3475 - Flagellar basal body rod protein:Protein of unknown function DUF1078, at 4,145,637 to 4,146,425
_3475
Psyr_3476: Psyr_3476 - Flagellar basal body rod protein:Protein of unknown function DUF1078, at 4,146,472 to 4,147,212
_3476
Psyr_3477: Psyr_3477 - Flagellar basal body rod protein:Protein of unknown function DUF1078:Flagellar basal body FlaE, at 4,147,688 to 4,149,436
_3477
Position (kb)
4146
4147
4148 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4145.575 kb on - strand at 4145.575 kb on - strand at 4145.639 kb on - strand at 4145.848 kb on + strand, within Psyr_3475 at 4146.025 kb on + strand, within Psyr_3475 at 4146.072 kb on - strand, within Psyr_3475 at 4146.085 kb on + strand, within Psyr_3475 at 4146.085 kb on + strand, within Psyr_3475 at 4146.289 kb on + strand, within Psyr_3475 at 4146.290 kb on - strand, within Psyr_3475 at 4146.409 kb on + strand at 4146.409 kb on + strand at 4146.409 kb on + strand at 4146.409 kb on + strand at 4146.409 kb on + strand at 4146.409 kb on + strand at 4146.409 kb on + strand at 4146.409 kb on + strand at 4146.409 kb on + strand at 4146.409 kb on + strand at 4146.409 kb on + strand at 4146.409 kb on + strand at 4146.410 kb on - strand at 4146.410 kb on - strand at 4146.410 kb on - strand at 4146.410 kb on - strand at 4146.410 kb on - strand at 4146.410 kb on - strand at 4146.410 kb on - strand at 4146.410 kb on - strand at 4146.410 kb on - strand at 4146.410 kb on - strand at 4146.410 kb on - strand at 4146.410 kb on - strand at 4146.411 kb on + strand at 4146.411 kb on + strand at 4146.411 kb on + strand at 4146.412 kb on - strand at 4146.412 kb on - strand at 4146.498 kb on + strand at 4146.917 kb on + strand, within Psyr_3476 at 4146.917 kb on + strand, within Psyr_3476 at 4146.917 kb on + strand, within Psyr_3476 at 4146.918 kb on - strand, within Psyr_3476 at 4147.196 kb on + strand at 4147.196 kb on + strand at 4147.246 kb on + strand at 4147.247 kb on - strand at 4147.299 kb on - strand at 4147.459 kb on - strand at 4147.580 kb on + strand at 4147.580 kb on + strand at 4147.580 kb on + strand at 4147.581 kb on - strand at 4147.583 kb on + strand at 4147.583 kb on + strand at 4147.584 kb on - strand at 4147.584 kb on - strand at 4147.584 kb on - strand at 4147.584 kb on - strand at 4147.584 kb on - strand at 4147.584 kb on - strand at 4147.847 kb on - strand at 4147.866 kb on + strand, within Psyr_3477 at 4147.866 kb on + strand, within Psyr_3477 at 4147.867 kb on - strand, within Psyr_3477 at 4147.907 kb on - strand, within Psyr_3477 at 4147.972 kb on + strand, within Psyr_3477 at 4147.972 kb on + strand, within Psyr_3477 at 4147.972 kb on + strand, within Psyr_3477 at 4147.973 kb on - strand, within Psyr_3477 at 4147.973 kb on - strand, within Psyr_3477
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 4,145,575 - +1.0 4,145,575 - +1.5 4,145,639 - -0.0 4,145,848 + Psyr_3475 0.27 +0.9 4,146,025 + Psyr_3475 0.49 +1.7 4,146,072 - Psyr_3475 0.55 -1.9 4,146,085 + Psyr_3475 0.57 +0.5 4,146,085 + Psyr_3475 0.57 +0.2 4,146,289 + Psyr_3475 0.83 -0.1 4,146,290 - Psyr_3475 0.83 -0.5 4,146,409 + +2.5 4,146,409 + -0.9 4,146,409 + +4.5 4,146,409 + -0.8 4,146,409 + -1.5 4,146,409 + +1.1 4,146,409 + +1.1 4,146,409 + -0.0 4,146,409 + -2.5 4,146,409 + -0.2 4,146,409 + -0.5 4,146,409 + -0.2 4,146,410 - -0.7 4,146,410 - -3.0 4,146,410 - -0.8 4,146,410 - +0.2 4,146,410 - -0.2 4,146,410 - +0.6 4,146,410 - +0.7 4,146,410 - -1.8 4,146,410 - +1.1 4,146,410 - +0.4 4,146,410 - -1.1 4,146,410 - +0.3 4,146,411 + -0.7 4,146,411 + +0.5 4,146,411 + -2.5 4,146,412 - +0.7 4,146,412 - -1.4 4,146,498 + -1.3 4,146,917 + Psyr_3476 0.60 -1.4 4,146,917 + Psyr_3476 0.60 -0.6 4,146,917 + Psyr_3476 0.60 +1.1 4,146,918 - Psyr_3476 0.60 -1.7 4,147,196 + +0.1 4,147,196 + -0.0 4,147,246 + -0.5 4,147,247 - +0.3 4,147,299 - +0.7 4,147,459 - -0.3 4,147,580 + +0.2 4,147,580 + -1.4 4,147,580 + -0.9 4,147,581 - +0.3 4,147,583 + -0.4 4,147,583 + +0.3 4,147,584 - +1.5 4,147,584 - +0.8 4,147,584 - +2.6 4,147,584 - +0.8 4,147,584 - -0.3 4,147,584 - -0.2 4,147,847 - -0.1 4,147,866 + Psyr_3477 0.10 +0.5 4,147,866 + Psyr_3477 0.10 -1.3 4,147,867 - Psyr_3477 0.10 -1.2 4,147,907 - Psyr_3477 0.13 -0.3 4,147,972 + Psyr_3477 0.16 -0.5 4,147,972 + Psyr_3477 0.16 +0.1 4,147,972 + Psyr_3477 0.16 -1.7 4,147,973 - Psyr_3477 0.16 +2.2 4,147,973 - Psyr_3477 0.16 -0.2
Or see this region's nucleotide sequence