Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3434 and Psyr_3435 are separated by 33 nucleotides Psyr_3435 and Psyr_3436 are separated by 19 nucleotides Psyr_3436 and Psyr_3437 are separated by 244 nucleotides
Psyr_3434: Psyr_3434 - CheW-like protein:ATP-binding region, ATPase-like:Signal transducing histidine kinase, homodimeric:Hpt, at 4,096,830 to 4,099,106
_3434
Psyr_3435: Psyr_3435 - chemotaxis phosphatase, CheZ, at 4,099,140 to 4,099,925
_3435
Psyr_3436: Psyr_3436 - Response regulator receiver, at 4,099,945 to 4,100,331
_3436
Psyr_3437: Psyr_3437 - RNA polymerase, sigma 28 subunit, SigD/FliA/WhiG, at 4,100,576 to 4,101,316
_3437
Position (kb)
4099
4100 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4098.670 kb on - strand, within Psyr_3434 at 4098.771 kb on + strand, within Psyr_3434 at 4098.789 kb on + strand, within Psyr_3434 at 4098.954 kb on + strand at 4098.954 kb on + strand at 4098.954 kb on + strand at 4098.955 kb on - strand at 4098.955 kb on - strand at 4098.955 kb on - strand at 4098.955 kb on - strand at 4098.955 kb on - strand at 4098.955 kb on - strand at 4098.955 kb on - strand at 4098.955 kb on - strand at 4098.955 kb on - strand at 4099.141 kb on + strand at 4099.141 kb on + strand at 4099.142 kb on - strand at 4099.142 kb on - strand at 4099.170 kb on + strand at 4099.170 kb on + strand at 4099.171 kb on - strand at 4099.171 kb on - strand at 4099.171 kb on - strand at 4099.206 kb on - strand at 4099.405 kb on + strand, within Psyr_3435 at 4099.598 kb on + strand, within Psyr_3435 at 4099.657 kb on + strand, within Psyr_3435 at 4099.657 kb on + strand, within Psyr_3435 at 4099.657 kb on + strand, within Psyr_3435 at 4099.657 kb on + strand, within Psyr_3435 at 4099.657 kb on + strand, within Psyr_3435 at 4099.658 kb on - strand, within Psyr_3435 at 4099.658 kb on - strand, within Psyr_3435 at 4099.734 kb on + strand, within Psyr_3435 at 4099.884 kb on + strand at 4099.950 kb on + strand at 4099.950 kb on + strand at 4099.950 kb on + strand at 4099.951 kb on - strand at 4099.951 kb on - strand at 4099.951 kb on - strand at 4100.014 kb on + strand, within Psyr_3436 at 4100.014 kb on + strand, within Psyr_3436 at 4100.014 kb on + strand, within Psyr_3436 at 4100.014 kb on + strand, within Psyr_3436 at 4100.014 kb on + strand, within Psyr_3436 at 4100.014 kb on + strand, within Psyr_3436 at 4100.014 kb on + strand, within Psyr_3436 at 4100.014 kb on + strand, within Psyr_3436 at 4100.014 kb on + strand, within Psyr_3436 at 4100.014 kb on + strand, within Psyr_3436 at 4100.015 kb on - strand, within Psyr_3436 at 4100.015 kb on - strand, within Psyr_3436 at 4100.015 kb on - strand, within Psyr_3436 at 4100.015 kb on - strand, within Psyr_3436 at 4100.018 kb on + strand, within Psyr_3436 at 4100.019 kb on - strand, within Psyr_3436 at 4100.019 kb on - strand, within Psyr_3436 at 4100.019 kb on - strand, within Psyr_3436 at 4100.244 kb on + strand, within Psyr_3436 at 4100.244 kb on + strand, within Psyr_3436 at 4100.245 kb on - strand, within Psyr_3436 at 4100.518 kb on + strand at 4100.559 kb on - strand at 4100.633 kb on + strand at 4100.633 kb on + strand at 4100.633 kb on + strand at 4100.634 kb on - strand at 4100.634 kb on - strand at 4100.634 kb on - strand at 4100.703 kb on + strand, within Psyr_3437 at 4100.704 kb on - strand, within Psyr_3437 at 4100.706 kb on + strand, within Psyr_3437 at 4100.706 kb on + strand, within Psyr_3437 at 4100.706 kb on + strand, within Psyr_3437 at 4100.707 kb on - strand, within Psyr_3437 at 4100.707 kb on - strand, within Psyr_3437 at 4100.893 kb on + strand, within Psyr_3437 at 4100.913 kb on + strand, within Psyr_3437 at 4100.913 kb on + strand, within Psyr_3437 at 4100.913 kb on + strand, within Psyr_3437 at 4100.913 kb on + strand, within Psyr_3437 at 4100.913 kb on + strand, within Psyr_3437 at 4100.914 kb on - strand, within Psyr_3437 at 4100.914 kb on - strand, within Psyr_3437 at 4100.914 kb on - strand, within Psyr_3437 at 4100.914 kb on - strand, within Psyr_3437
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 4,098,670 - Psyr_3434 0.81 -0.6 4,098,771 + Psyr_3434 0.85 -2.6 4,098,789 + Psyr_3434 0.86 +0.2 4,098,954 + +0.6 4,098,954 + -0.1 4,098,954 + -1.7 4,098,955 - +0.1 4,098,955 - -0.9 4,098,955 - -0.7 4,098,955 - +0.3 4,098,955 - -0.5 4,098,955 - -0.2 4,098,955 - -0.2 4,098,955 - +0.2 4,098,955 - +0.1 4,099,141 + -1.2 4,099,141 + +0.1 4,099,142 - +0.0 4,099,142 - -0.7 4,099,170 + -1.5 4,099,170 + -2.8 4,099,171 - -0.2 4,099,171 - -0.6 4,099,171 - +0.1 4,099,206 - -1.2 4,099,405 + Psyr_3435 0.34 -2.5 4,099,598 + Psyr_3435 0.58 -0.4 4,099,657 + Psyr_3435 0.66 -0.9 4,099,657 + Psyr_3435 0.66 +0.5 4,099,657 + Psyr_3435 0.66 -0.5 4,099,657 + Psyr_3435 0.66 -0.4 4,099,657 + Psyr_3435 0.66 -1.3 4,099,658 - Psyr_3435 0.66 -1.5 4,099,658 - Psyr_3435 0.66 -0.1 4,099,734 + Psyr_3435 0.76 -0.2 4,099,884 + -0.6 4,099,950 + -0.4 4,099,950 + +1.7 4,099,950 + +1.1 4,099,951 - -0.6 4,099,951 - -0.5 4,099,951 - -1.1 4,100,014 + Psyr_3436 0.18 +0.7 4,100,014 + Psyr_3436 0.18 -0.5 4,100,014 + Psyr_3436 0.18 -0.6 4,100,014 + Psyr_3436 0.18 -1.5 4,100,014 + Psyr_3436 0.18 -1.0 4,100,014 + Psyr_3436 0.18 -1.6 4,100,014 + Psyr_3436 0.18 +0.7 4,100,014 + Psyr_3436 0.18 +0.1 4,100,014 + Psyr_3436 0.18 +0.7 4,100,014 + Psyr_3436 0.18 +0.7 4,100,015 - Psyr_3436 0.18 -0.3 4,100,015 - Psyr_3436 0.18 +1.5 4,100,015 - Psyr_3436 0.18 -2.4 4,100,015 - Psyr_3436 0.18 -2.8 4,100,018 + Psyr_3436 0.19 +1.9 4,100,019 - Psyr_3436 0.19 +2.5 4,100,019 - Psyr_3436 0.19 +0.7 4,100,019 - Psyr_3436 0.19 -0.0 4,100,244 + Psyr_3436 0.77 +2.0 4,100,244 + Psyr_3436 0.77 -1.0 4,100,245 - Psyr_3436 0.78 -0.5 4,100,518 + +0.0 4,100,559 - +1.7 4,100,633 + -1.7 4,100,633 + +0.6 4,100,633 + +0.2 4,100,634 - -0.3 4,100,634 - +0.0 4,100,634 - +0.4 4,100,703 + Psyr_3437 0.17 +0.3 4,100,704 - Psyr_3437 0.17 -0.9 4,100,706 + Psyr_3437 0.18 -1.9 4,100,706 + Psyr_3437 0.18 -0.4 4,100,706 + Psyr_3437 0.18 +0.6 4,100,707 - Psyr_3437 0.18 +0.1 4,100,707 - Psyr_3437 0.18 -0.5 4,100,893 + Psyr_3437 0.43 +1.9 4,100,913 + Psyr_3437 0.45 +0.9 4,100,913 + Psyr_3437 0.45 -1.8 4,100,913 + Psyr_3437 0.45 -2.5 4,100,913 + Psyr_3437 0.45 +1.1 4,100,913 + Psyr_3437 0.45 +0.5 4,100,914 - Psyr_3437 0.46 +0.8 4,100,914 - Psyr_3437 0.46 +0.3 4,100,914 - Psyr_3437 0.46 -0.3 4,100,914 - Psyr_3437 0.46 -1.5
Or see this region's nucleotide sequence