Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3081 and Psyr_3082 are separated by 16 nucleotides Psyr_3082 and Psyr_3083 are separated by 19 nucleotides Psyr_3083 and Psyr_3084 are separated by 98 nucleotides Psyr_3084 and Psyr_3085 are separated by 5 nucleotides
Psyr_3081: Psyr_3081 - Secretion protein HlyD, at 3,686,678 to 3,687,811
_3081
Psyr_3082: Psyr_3082 - MltA-interacting MipA, at 3,687,828 to 3,688,676
_3082
Psyr_3083: Psyr_3083 - RND efflux system, outer membrane lipoprotein, NodT, at 3,688,696 to 3,690,168
_3083
Psyr_3084: Psyr_3084 - Response regulator receiver:Transcriptional regulatory protein, C-terminal, at 3,690,267 to 3,690,956
_3084
Psyr_3085: Psyr_3085 - ATP-binding region, ATPase-like:Histidine kinase, HAMP region:Histidine kinase A, N-terminal, at 3,690,962 to 3,692,137
_3085
Position (kb)
3688
3689
3690
3691 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3687.746 kb on + strand at 3687.839 kb on - strand at 3687.911 kb on - strand at 3687.959 kb on + strand, within Psyr_3082 at 3687.959 kb on + strand, within Psyr_3082 at 3687.960 kb on - strand, within Psyr_3082 at 3687.960 kb on - strand, within Psyr_3082 at 3687.960 kb on - strand, within Psyr_3082 at 3687.991 kb on + strand, within Psyr_3082 at 3688.061 kb on - strand, within Psyr_3082 at 3688.073 kb on - strand, within Psyr_3082 at 3688.073 kb on - strand, within Psyr_3082 at 3688.275 kb on - strand, within Psyr_3082 at 3688.483 kb on + strand, within Psyr_3082 at 3688.631 kb on + strand at 3688.632 kb on - strand at 3688.656 kb on - strand at 3689.053 kb on - strand, within Psyr_3083 at 3689.171 kb on - strand, within Psyr_3083 at 3689.331 kb on + strand, within Psyr_3083 at 3689.654 kb on + strand, within Psyr_3083 at 3690.000 kb on + strand, within Psyr_3083 at 3690.001 kb on - strand, within Psyr_3083 at 3690.001 kb on - strand, within Psyr_3083 at 3690.156 kb on + strand at 3690.169 kb on - strand at 3690.239 kb on + strand at 3690.240 kb on - strand at 3690.330 kb on + strand at 3690.330 kb on + strand at 3690.331 kb on - strand at 3690.549 kb on + strand, within Psyr_3084 at 3690.564 kb on + strand, within Psyr_3084 at 3690.564 kb on + strand, within Psyr_3084 at 3690.564 kb on + strand, within Psyr_3084 at 3690.565 kb on - strand, within Psyr_3084 at 3690.580 kb on - strand, within Psyr_3084 at 3690.955 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 3,687,746 + +0.9 3,687,839 - -1.2 3,687,911 - +0.0 3,687,959 + Psyr_3082 0.15 -0.1 3,687,959 + Psyr_3082 0.15 +0.8 3,687,960 - Psyr_3082 0.16 +0.4 3,687,960 - Psyr_3082 0.16 +0.1 3,687,960 - Psyr_3082 0.16 +0.1 3,687,991 + Psyr_3082 0.19 -0.5 3,688,061 - Psyr_3082 0.27 -0.7 3,688,073 - Psyr_3082 0.29 +1.5 3,688,073 - Psyr_3082 0.29 +0.9 3,688,275 - Psyr_3082 0.53 -0.4 3,688,483 + Psyr_3082 0.77 +0.3 3,688,631 + +0.7 3,688,632 - +0.4 3,688,656 - -0.7 3,689,053 - Psyr_3083 0.24 -0.5 3,689,171 - Psyr_3083 0.32 +0.5 3,689,331 + Psyr_3083 0.43 -1.9 3,689,654 + Psyr_3083 0.65 +1.4 3,690,000 + Psyr_3083 0.89 +2.2 3,690,001 - Psyr_3083 0.89 +1.1 3,690,001 - Psyr_3083 0.89 -0.6 3,690,156 + -0.4 3,690,169 - -0.6 3,690,239 + -0.4 3,690,240 - -0.5 3,690,330 + -0.5 3,690,330 + +1.1 3,690,331 - -0.1 3,690,549 + Psyr_3084 0.41 +0.3 3,690,564 + Psyr_3084 0.43 +2.4 3,690,564 + Psyr_3084 0.43 +0.6 3,690,564 + Psyr_3084 0.43 -1.5 3,690,565 - Psyr_3084 0.43 -2.1 3,690,580 - Psyr_3084 0.45 -0.2 3,690,955 - -0.3
Or see this region's nucleotide sequence