Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3014 and Psyr_3015 overlap by 1 nucleotides Psyr_3015 and Psyr_3016 are separated by 3 nucleotides
Psyr_3014: Psyr_3014 - protoporphyrin IX magnesium-chelatase, at 3,607,504 to 3,608,538
_3014
Psyr_3015: Psyr_3015 - cobaltochelatase CobN subunit, at 3,608,538 to 3,612,302
_3015
Psyr_3016: Psyr_3016 - Cobalamin synthesis protein/P47K:Cobalamin synthesis protein/P47K, at 3,612,306 to 3,613,370
_3016
Position (kb)
3608
3609
3610
3611
3612
3613 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 3608.210 kb on + strand, within Psyr_3014 at 3608.210 kb on + strand, within Psyr_3014 at 3608.210 kb on + strand, within Psyr_3014 at 3608.210 kb on + strand, within Psyr_3014 at 3608.210 kb on + strand, within Psyr_3014 at 3608.211 kb on - strand, within Psyr_3014 at 3608.211 kb on - strand, within Psyr_3014 at 3608.211 kb on - strand, within Psyr_3014 at 3608.550 kb on + strand at 3608.550 kb on + strand at 3608.550 kb on + strand at 3608.550 kb on + strand at 3608.550 kb on + strand at 3608.550 kb on + strand at 3608.551 kb on - strand at 3608.551 kb on - strand at 3608.551 kb on - strand at 3608.551 kb on - strand at 3608.551 kb on - strand at 3608.551 kb on - strand at 3608.551 kb on - strand at 3608.704 kb on + strand at 3608.810 kb on - strand at 3608.998 kb on + strand, within Psyr_3015 at 3608.998 kb on + strand, within Psyr_3015 at 3608.999 kb on - strand, within Psyr_3015 at 3609.119 kb on - strand, within Psyr_3015 at 3609.199 kb on - strand, within Psyr_3015 at 3609.212 kb on - strand, within Psyr_3015 at 3609.958 kb on + strand, within Psyr_3015 at 3610.211 kb on + strand, within Psyr_3015 at 3610.211 kb on + strand, within Psyr_3015 at 3610.212 kb on - strand, within Psyr_3015 at 3610.212 kb on - strand, within Psyr_3015 at 3610.255 kb on + strand, within Psyr_3015 at 3610.255 kb on + strand, within Psyr_3015 at 3610.256 kb on - strand, within Psyr_3015 at 3610.256 kb on - strand, within Psyr_3015 at 3610.256 kb on - strand, within Psyr_3015 at 3610.256 kb on - strand, within Psyr_3015 at 3610.412 kb on - strand, within Psyr_3015 at 3610.454 kb on - strand at 3610.559 kb on - strand, within Psyr_3015 at 3610.702 kb on + strand, within Psyr_3015 at 3610.703 kb on - strand, within Psyr_3015 at 3610.703 kb on - strand, within Psyr_3015 at 3610.703 kb on - strand, within Psyr_3015 at 3610.703 kb on - strand, within Psyr_3015 at 3610.747 kb on + strand, within Psyr_3015 at 3610.930 kb on + strand, within Psyr_3015 at 3610.931 kb on - strand, within Psyr_3015 at 3611.767 kb on + strand, within Psyr_3015 at 3611.767 kb on + strand, within Psyr_3015 at 3611.767 kb on + strand, within Psyr_3015 at 3611.768 kb on - strand, within Psyr_3015 at 3611.768 kb on - strand, within Psyr_3015 at 3611.768 kb on - strand, within Psyr_3015 at 3611.845 kb on + strand, within Psyr_3015 at 3611.845 kb on + strand, within Psyr_3015 at 3611.846 kb on - strand, within Psyr_3015 at 3611.846 kb on - strand, within Psyr_3015 at 3611.846 kb on - strand, within Psyr_3015 at 3611.846 kb on - strand, within Psyr_3015 at 3612.091 kb on + strand at 3612.092 kb on - strand at 3612.092 kb on - strand at 3612.092 kb on - strand at 3612.152 kb on - strand at 3612.254 kb on + strand at 3612.254 kb on + strand at 3612.255 kb on - strand at 3612.294 kb on + strand at 3613.163 kb on - strand, within Psyr_3016 at 3613.163 kb on - strand, within Psyr_3016
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 3,608,210 + Psyr_3014 0.68 +0.6 3,608,210 + Psyr_3014 0.68 -0.4 3,608,210 + Psyr_3014 0.68 +0.1 3,608,210 + Psyr_3014 0.68 -0.6 3,608,210 + Psyr_3014 0.68 -2.9 3,608,211 - Psyr_3014 0.68 -0.7 3,608,211 - Psyr_3014 0.68 -0.9 3,608,211 - Psyr_3014 0.68 -2.1 3,608,550 + -1.2 3,608,550 + +0.3 3,608,550 + +1.7 3,608,550 + -0.4 3,608,550 + -0.7 3,608,550 + +1.1 3,608,551 - +3.4 3,608,551 - +1.1 3,608,551 - -0.7 3,608,551 - -1.9 3,608,551 - +1.2 3,608,551 - -0.9 3,608,551 - -0.6 3,608,704 + +0.4 3,608,810 - +1.7 3,608,998 + Psyr_3015 0.12 -0.2 3,608,998 + Psyr_3015 0.12 -2.8 3,608,999 - Psyr_3015 0.12 +1.5 3,609,119 - Psyr_3015 0.15 -0.2 3,609,199 - Psyr_3015 0.18 +1.0 3,609,212 - Psyr_3015 0.18 -0.1 3,609,958 + Psyr_3015 0.38 +2.4 3,610,211 + Psyr_3015 0.44 -1.2 3,610,211 + Psyr_3015 0.44 +0.1 3,610,212 - Psyr_3015 0.44 -3.1 3,610,212 - Psyr_3015 0.44 -0.5 3,610,255 + Psyr_3015 0.46 -1.2 3,610,255 + Psyr_3015 0.46 -1.9 3,610,256 - Psyr_3015 0.46 -0.6 3,610,256 - Psyr_3015 0.46 -1.9 3,610,256 - Psyr_3015 0.46 -0.7 3,610,256 - Psyr_3015 0.46 -1.2 3,610,412 - Psyr_3015 0.50 -1.1 3,610,454 - +0.5 3,610,559 - Psyr_3015 0.54 -1.9 3,610,702 + Psyr_3015 0.57 -1.0 3,610,703 - Psyr_3015 0.58 -0.9 3,610,703 - Psyr_3015 0.58 -3.4 3,610,703 - Psyr_3015 0.58 -0.5 3,610,703 - Psyr_3015 0.58 +1.4 3,610,747 + Psyr_3015 0.59 +1.1 3,610,930 + Psyr_3015 0.64 -0.3 3,610,931 - Psyr_3015 0.64 -2.2 3,611,767 + Psyr_3015 0.86 -0.9 3,611,767 + Psyr_3015 0.86 +0.6 3,611,767 + Psyr_3015 0.86 -2.4 3,611,768 - Psyr_3015 0.86 -0.5 3,611,768 - Psyr_3015 0.86 +5.4 3,611,768 - Psyr_3015 0.86 +0.7 3,611,845 + Psyr_3015 0.88 -0.0 3,611,845 + Psyr_3015 0.88 -1.3 3,611,846 - Psyr_3015 0.88 +0.5 3,611,846 - Psyr_3015 0.88 +0.6 3,611,846 - Psyr_3015 0.88 -0.2 3,611,846 - Psyr_3015 0.88 +0.3 3,612,091 + +0.1 3,612,092 - +0.1 3,612,092 - -1.4 3,612,092 - +3.7 3,612,152 - -0.5 3,612,254 + +0.3 3,612,254 + -1.2 3,612,255 - -1.1 3,612,294 + -2.0 3,613,163 - Psyr_3016 0.80 -0.4 3,613,163 - Psyr_3016 0.80 -0.7
Or see this region's nucleotide sequence