Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2871 and Psyr_2872 are separated by 38 nucleotides Psyr_2872 and Psyr_2873 are separated by 148 nucleotides Psyr_2873 and Psyr_2874 are separated by 7 nucleotides Psyr_2874 and Psyr_2875 overlap by 4 nucleotides
Psyr_2871: Psyr_2871 - CDS, at 3,445,461 to 3,446,675
_2871
Psyr_2872: Psyr_2872 - hypothetical protein, at 3,446,714 to 3,446,953
_2872
Psyr_2873: Psyr_2873 - Acyl-CoA dehydrogenase, C-terminal, at 3,447,102 to 3,448,340
_2873
Psyr_2874: Psyr_2874 - monosaccharide ABC transporter membrane protein, CUT2 family, at 3,448,348 to 3,449,334
_2874
Psyr_2875: Psyr_2875 - monosaccharide ABC transporter membrane protein, CUT2 family, at 3,449,331 to 3,450,335
_2875
Position (kb)
3447
3448
3449 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3446.186 kb on - strand, within Psyr_2871 at 3446.317 kb on + strand, within Psyr_2871 at 3446.317 kb on + strand, within Psyr_2871 at 3446.446 kb on + strand, within Psyr_2871 at 3446.446 kb on + strand, within Psyr_2871 at 3446.446 kb on + strand, within Psyr_2871 at 3446.446 kb on + strand, within Psyr_2871 at 3446.446 kb on + strand, within Psyr_2871 at 3446.447 kb on - strand, within Psyr_2871 at 3446.447 kb on - strand, within Psyr_2871 at 3446.447 kb on - strand, within Psyr_2871 at 3446.484 kb on - strand, within Psyr_2871 at 3446.888 kb on + strand, within Psyr_2872 at 3446.888 kb on + strand, within Psyr_2872 at 3446.889 kb on - strand, within Psyr_2872 at 3447.096 kb on + strand at 3447.573 kb on + strand, within Psyr_2873 at 3447.576 kb on + strand, within Psyr_2873 at 3447.577 kb on - strand, within Psyr_2873 at 3447.577 kb on - strand, within Psyr_2873 at 3447.577 kb on - strand, within Psyr_2873 at 3447.615 kb on + strand, within Psyr_2873 at 3447.616 kb on - strand, within Psyr_2873 at 3447.616 kb on - strand, within Psyr_2873 at 3447.616 kb on - strand, within Psyr_2873 at 3447.799 kb on - strand, within Psyr_2873 at 3448.032 kb on + strand, within Psyr_2873 at 3448.033 kb on - strand at 3448.143 kb on - strand, within Psyr_2873 at 3448.251 kb on + strand at 3448.251 kb on + strand at 3448.252 kb on - strand at 3448.252 kb on - strand at 3448.349 kb on + strand at 3448.349 kb on + strand at 3448.436 kb on + strand at 3448.703 kb on + strand, within Psyr_2874 at 3448.704 kb on - strand, within Psyr_2874 at 3448.706 kb on + strand, within Psyr_2874 at 3448.707 kb on - strand, within Psyr_2874 at 3448.707 kb on - strand, within Psyr_2874 at 3448.871 kb on + strand, within Psyr_2874 at 3448.872 kb on - strand, within Psyr_2874 at 3448.872 kb on - strand, within Psyr_2874 at 3448.872 kb on - strand, within Psyr_2874 at 3448.872 kb on - strand, within Psyr_2874 at 3448.872 kb on - strand, within Psyr_2874 at 3448.872 kb on - strand, within Psyr_2874 at 3449.104 kb on + strand at 3449.328 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 3,446,186 - Psyr_2871 0.60 +0.4 3,446,317 + Psyr_2871 0.70 -0.6 3,446,317 + Psyr_2871 0.70 +0.0 3,446,446 + Psyr_2871 0.81 +1.8 3,446,446 + Psyr_2871 0.81 +0.3 3,446,446 + Psyr_2871 0.81 +1.0 3,446,446 + Psyr_2871 0.81 +1.2 3,446,446 + Psyr_2871 0.81 +0.5 3,446,447 - Psyr_2871 0.81 +0.6 3,446,447 - Psyr_2871 0.81 -1.7 3,446,447 - Psyr_2871 0.81 -0.3 3,446,484 - Psyr_2871 0.84 +0.4 3,446,888 + Psyr_2872 0.72 -1.0 3,446,888 + Psyr_2872 0.72 +2.8 3,446,889 - Psyr_2872 0.73 -0.2 3,447,096 + -0.3 3,447,573 + Psyr_2873 0.38 -1.3 3,447,576 + Psyr_2873 0.38 -1.5 3,447,577 - Psyr_2873 0.38 -1.6 3,447,577 - Psyr_2873 0.38 -3.4 3,447,577 - Psyr_2873 0.38 -1.5 3,447,615 + Psyr_2873 0.41 +0.9 3,447,616 - Psyr_2873 0.41 +1.2 3,447,616 - Psyr_2873 0.41 +1.8 3,447,616 - Psyr_2873 0.41 -0.8 3,447,799 - Psyr_2873 0.56 +0.3 3,448,032 + Psyr_2873 0.75 -1.8 3,448,033 - -0.8 3,448,143 - Psyr_2873 0.84 -1.8 3,448,251 + +1.0 3,448,251 + -1.6 3,448,252 - -1.8 3,448,252 - +0.9 3,448,349 + -0.1 3,448,349 + +3.2 3,448,436 + -1.3 3,448,703 + Psyr_2874 0.36 -0.0 3,448,704 - Psyr_2874 0.36 +2.6 3,448,706 + Psyr_2874 0.36 -0.1 3,448,707 - Psyr_2874 0.36 +1.2 3,448,707 - Psyr_2874 0.36 -0.3 3,448,871 + Psyr_2874 0.53 -1.0 3,448,872 - Psyr_2874 0.53 -1.3 3,448,872 - Psyr_2874 0.53 +1.5 3,448,872 - Psyr_2874 0.53 +0.3 3,448,872 - Psyr_2874 0.53 -0.9 3,448,872 - Psyr_2874 0.53 -0.1 3,448,872 - Psyr_2874 0.53 -1.0 3,449,104 + -0.4 3,449,328 + -1.2
Or see this region's nucleotide sequence