Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2817 and Psyr_2818 are separated by 20 nucleotides Psyr_2818 and Psyr_2819 overlap by 4 nucleotides Psyr_2819 and Psyr_2820 are separated by 329 nucleotides Psyr_2820 and Psyr_2821 overlap by 4 nucleotides
Psyr_2817: Psyr_2817 - hypothetical protein, at 3,392,815 to 3,393,282
_2817
Psyr_2818: Psyr_2818 - hypothetical protein, at 3,393,303 to 3,393,710
_2818
Psyr_2819: Psyr_2819 - hypothetical protein, at 3,393,707 to 3,393,898
_2819
Psyr_2820: Psyr_2820 - RecT protein, at 3,394,228 to 3,395,115
_2820
Psyr_2821: Psyr_2821 - conserved hypothetical protein, at 3,395,112 to 3,396,773
_2821
Position (kb) 3394
3395
3396 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.265 kb on - strand at 3393.265 kb on - strand at 3393.549 kb on + strand, within Psyr_2818 at 3393.549 kb on + strand, within Psyr_2818 at 3393.549 kb on + strand, within Psyr_2818 at 3393.549 kb on + strand, within Psyr_2818 at 3393.549 kb on + strand, within Psyr_2818 at 3393.549 kb on + strand, within Psyr_2818 at 3393.549 kb on + strand, within Psyr_2818 at 3393.550 kb on - strand, within Psyr_2818 at 3393.550 kb on - strand, within Psyr_2818 at 3393.550 kb on - strand, within Psyr_2818 at 3393.550 kb on - strand, within Psyr_2818 at 3393.980 kb on - strand at 3394.092 kb on + strand at 3394.092 kb on + strand at 3394.092 kb on + strand at 3394.093 kb on - strand at 3394.093 kb on - strand at 3394.093 kb on - strand at 3394.093 kb on - strand at 3394.177 kb on - strand at 3394.227 kb on + strand at 3394.227 kb on + strand at 3394.228 kb on - strand at 3394.316 kb on + strand at 3394.534 kb on - strand, within Psyr_2820 at 3394.831 kb on + strand, within Psyr_2820 at 3394.832 kb on - strand, within Psyr_2820 at 3394.945 kb on + strand, within Psyr_2820 at 3395.047 kb on + strand at 3395.048 kb on - strand at 3395.048 kb on - strand at 3395.123 kb on + strand at 3395.169 kb on + strand at 3395.170 kb on - strand at 3395.289 kb on + strand, within Psyr_2821 at 3395.289 kb on + strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.502 kb on + strand, within Psyr_2821 at 3395.502 kb on + strand, within Psyr_2821 at 3395.503 kb on - strand, within Psyr_2821 at 3395.503 kb on - strand, within Psyr_2821 at 3395.503 kb on - strand, within Psyr_2821 at 3395.505 kb on + strand, within Psyr_2821 at 3395.505 kb on + strand, within Psyr_2821 at 3395.602 kb on - strand, within Psyr_2821 at 3395.674 kb on - strand, within Psyr_2821 at 3395.747 kb on - strand, within Psyr_2821
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 3,393,264 + -1.8 3,393,264 + -1.1 3,393,264 + +0.6 3,393,264 + -2.1 3,393,264 + -0.9 3,393,264 + -1.6 3,393,264 + +0.8 3,393,264 + -1.6 3,393,265 - -1.6 3,393,265 - -1.2 3,393,549 + Psyr_2818 0.60 -0.6 3,393,549 + Psyr_2818 0.60 -0.6 3,393,549 + Psyr_2818 0.60 -1.0 3,393,549 + Psyr_2818 0.60 -0.7 3,393,549 + Psyr_2818 0.60 -1.6 3,393,549 + Psyr_2818 0.60 -0.7 3,393,549 + Psyr_2818 0.60 -0.1 3,393,550 - Psyr_2818 0.61 +0.1 3,393,550 - Psyr_2818 0.61 +0.2 3,393,550 - Psyr_2818 0.61 +1.9 3,393,550 - Psyr_2818 0.61 +0.5 3,393,980 - -1.6 3,394,092 + -0.8 3,394,092 + -0.8 3,394,092 + -2.3 3,394,093 - +0.3 3,394,093 - +0.4 3,394,093 - +0.4 3,394,093 - +0.5 3,394,177 - +0.1 3,394,227 + -0.3 3,394,227 + -1.8 3,394,228 - +0.6 3,394,316 + +0.7 3,394,534 - Psyr_2820 0.34 -3.3 3,394,831 + Psyr_2820 0.68 -0.4 3,394,832 - Psyr_2820 0.68 -1.0 3,394,945 + Psyr_2820 0.81 -0.7 3,395,047 + -0.8 3,395,048 - -0.5 3,395,048 - -0.3 3,395,123 + +1.9 3,395,169 + +4.0 3,395,170 - +0.2 3,395,289 + Psyr_2821 0.11 -0.7 3,395,289 + Psyr_2821 0.11 -1.2 3,395,290 - Psyr_2821 0.11 -0.0 3,395,290 - Psyr_2821 0.11 +1.2 3,395,290 - Psyr_2821 0.11 -0.6 3,395,290 - Psyr_2821 0.11 +2.8 3,395,290 - Psyr_2821 0.11 -1.4 3,395,290 - Psyr_2821 0.11 -0.7 3,395,502 + Psyr_2821 0.23 +0.9 3,395,502 + Psyr_2821 0.23 -1.3 3,395,503 - Psyr_2821 0.24 -2.3 3,395,503 - Psyr_2821 0.24 +0.2 3,395,503 - Psyr_2821 0.24 -0.1 3,395,505 + Psyr_2821 0.24 +3.0 3,395,505 + Psyr_2821 0.24 +0.1 3,395,602 - Psyr_2821 0.29 +0.1 3,395,674 - Psyr_2821 0.34 -2.2 3,395,747 - Psyr_2821 0.38 +0.3
Or see this region's nucleotide sequence