Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2592 and Psyr_2593 are separated by 10 nucleotides Psyr_2593 and Psyr_2594 overlap by 4 nucleotides Psyr_2594 and Psyr_2595 overlap by 4 nucleotides
Psyr_2592: Psyr_2592 - transport system permease protein, at 3,038,676 to 3,039,707
_2592
Psyr_2593: Psyr_2593 - ABC transporter, at 3,039,718 to 3,040,509
_2593
Psyr_2594: Psyr_2594 - unknown conserved protein in bacilli, at 3,040,506 to 3,041,285
_2594
Psyr_2595: Psyr_2595 - Dimethylmenaquinone methyltransferase, at 3,041,282 to 3,041,926
_2595
Position (kb)
3039
3040
3041 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3038.779 kb on + strand at 3039.031 kb on - strand, within Psyr_2592 at 3039.031 kb on - strand, within Psyr_2592 at 3039.031 kb on - strand, within Psyr_2592 at 3039.031 kb on - strand, within Psyr_2592 at 3039.031 kb on - strand, within Psyr_2592 at 3039.118 kb on - strand, within Psyr_2592 at 3039.118 kb on - strand, within Psyr_2592 at 3039.249 kb on + strand, within Psyr_2592 at 3039.249 kb on + strand, within Psyr_2592 at 3039.249 kb on + strand, within Psyr_2592 at 3039.250 kb on - strand, within Psyr_2592 at 3039.273 kb on + strand, within Psyr_2592 at 3039.273 kb on + strand, within Psyr_2592 at 3039.273 kb on + strand, within Psyr_2592 at 3039.273 kb on + strand, within Psyr_2592 at 3039.274 kb on - strand, within Psyr_2592 at 3039.274 kb on - strand, within Psyr_2592 at 3039.682 kb on - strand at 3039.685 kb on + strand at 3039.686 kb on - strand at 3039.705 kb on + strand at 3039.713 kb on + strand at 3039.714 kb on - strand at 3039.714 kb on - strand at 3040.199 kb on + strand, within Psyr_2593 at 3040.199 kb on + strand, within Psyr_2593 at 3040.199 kb on + strand, within Psyr_2593 at 3040.199 kb on + strand, within Psyr_2593 at 3040.199 kb on + strand, within Psyr_2593 at 3040.200 kb on - strand, within Psyr_2593 at 3040.200 kb on - strand, within Psyr_2593 at 3040.200 kb on - strand at 3040.200 kb on - strand, within Psyr_2593 at 3040.200 kb on - strand, within Psyr_2593 at 3040.867 kb on - strand, within Psyr_2594 at 3041.002 kb on + strand, within Psyr_2594 at 3041.010 kb on + strand, within Psyr_2594 at 3041.127 kb on + strand, within Psyr_2594 at 3041.215 kb on - strand at 3041.217 kb on + strand at 3041.340 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 3,038,779 + -0.4 3,039,031 - Psyr_2592 0.34 +1.2 3,039,031 - Psyr_2592 0.34 -2.4 3,039,031 - Psyr_2592 0.34 -0.7 3,039,031 - Psyr_2592 0.34 -3.3 3,039,031 - Psyr_2592 0.34 -2.5 3,039,118 - Psyr_2592 0.43 -1.8 3,039,118 - Psyr_2592 0.43 -1.1 3,039,249 + Psyr_2592 0.56 +1.6 3,039,249 + Psyr_2592 0.56 -0.4 3,039,249 + Psyr_2592 0.56 -2.6 3,039,250 - Psyr_2592 0.56 -0.8 3,039,273 + Psyr_2592 0.58 -3.2 3,039,273 + Psyr_2592 0.58 -1.6 3,039,273 + Psyr_2592 0.58 +0.7 3,039,273 + Psyr_2592 0.58 +1.2 3,039,274 - Psyr_2592 0.58 -2.5 3,039,274 - Psyr_2592 0.58 -0.6 3,039,682 - -2.5 3,039,685 + +0.4 3,039,686 - -1.8 3,039,705 + +0.6 3,039,713 + -1.0 3,039,714 - -1.8 3,039,714 - -0.6 3,040,199 + Psyr_2593 0.61 -0.7 3,040,199 + Psyr_2593 0.61 -1.4 3,040,199 + Psyr_2593 0.61 +0.4 3,040,199 + Psyr_2593 0.61 -1.0 3,040,199 + Psyr_2593 0.61 -1.9 3,040,200 - Psyr_2593 0.61 -0.1 3,040,200 - Psyr_2593 0.61 -2.0 3,040,200 - +1.2 3,040,200 - Psyr_2593 0.61 +1.2 3,040,200 - Psyr_2593 0.61 -1.4 3,040,867 - Psyr_2594 0.46 -0.4 3,041,002 + Psyr_2594 0.64 -1.6 3,041,010 + Psyr_2594 0.65 -2.3 3,041,127 + Psyr_2594 0.80 -0.4 3,041,215 - -1.4 3,041,217 + -0.4 3,041,340 - -1.5
Or see this region's nucleotide sequence