Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2351 and Psyr_2352 are separated by 289 nucleotides Psyr_2352 and Psyr_2353 are separated by 115 nucleotides Psyr_2353 and Psyr_2354 are separated by 82 nucleotides
Psyr_2351: Psyr_2351 - tRNA synthetase, class-II (G, H, P and S), at 2,727,077 to 2,727,658
_2351
Psyr_2352: Psyr_2352 - GCN5-related N-acetyltransferase, at 2,727,948 to 2,728,475
_2352
Psyr_2353: Psyr_2353 - Uncharacterized protein conserved in bacteria-like protein, at 2,728,591 to 2,729,817
_2353
Psyr_2354: Psyr_2354 - Taurine catabolism dioxygenase TauD/TfdA, at 2,729,900 to 2,730,739
_2354
Position (kb)
2728
2729
2730 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2727.631 kb on + strand at 2727.631 kb on + strand at 2727.632 kb on - strand at 2727.632 kb on - strand at 2727.632 kb on - strand at 2727.702 kb on + strand at 2727.743 kb on + strand at 2727.926 kb on + strand at 2727.926 kb on + strand at 2727.949 kb on + strand at 2727.950 kb on - strand at 2728.003 kb on + strand, within Psyr_2352 at 2728.075 kb on + strand, within Psyr_2352 at 2728.075 kb on + strand, within Psyr_2352 at 2728.075 kb on + strand, within Psyr_2352 at 2728.075 kb on + strand, within Psyr_2352 at 2728.076 kb on - strand, within Psyr_2352 at 2728.076 kb on - strand, within Psyr_2352 at 2728.225 kb on + strand, within Psyr_2352 at 2728.225 kb on + strand, within Psyr_2352 at 2728.378 kb on + strand, within Psyr_2352 at 2728.378 kb on + strand, within Psyr_2352 at 2728.378 kb on + strand, within Psyr_2352 at 2728.378 kb on + strand, within Psyr_2352 at 2728.379 kb on - strand, within Psyr_2352 at 2728.379 kb on - strand, within Psyr_2352 at 2728.379 kb on - strand, within Psyr_2352 at 2728.379 kb on - strand, within Psyr_2352 at 2728.475 kb on + strand at 2728.475 kb on + strand at 2728.495 kb on + strand at 2728.495 kb on + strand at 2728.495 kb on + strand at 2728.496 kb on - strand at 2728.530 kb on + strand at 2728.530 kb on + strand at 2728.531 kb on - strand at 2728.531 kb on - strand at 2728.531 kb on - strand at 2728.565 kb on + strand at 2728.565 kb on + strand at 2728.565 kb on + strand at 2728.565 kb on + strand at 2728.566 kb on - strand at 2728.566 kb on - strand at 2728.566 kb on - strand at 2728.566 kb on - strand at 2728.566 kb on - strand at 2728.570 kb on + strand at 2728.571 kb on - strand at 2728.571 kb on - strand at 2728.571 kb on - strand at 2728.574 kb on + strand at 2728.575 kb on - strand at 2728.934 kb on + strand, within Psyr_2353 at 2728.987 kb on + strand, within Psyr_2353 at 2728.987 kb on + strand, within Psyr_2353 at 2728.988 kb on - strand, within Psyr_2353 at 2728.988 kb on - strand, within Psyr_2353 at 2728.991 kb on + strand, within Psyr_2353 at 2728.991 kb on + strand, within Psyr_2353 at 2728.991 kb on + strand, within Psyr_2353 at 2728.991 kb on + strand, within Psyr_2353 at 2728.991 kb on + strand, within Psyr_2353 at 2728.992 kb on - strand, within Psyr_2353 at 2728.992 kb on - strand, within Psyr_2353 at 2728.992 kb on - strand, within Psyr_2353 at 2728.992 kb on - strand, within Psyr_2353 at 2728.992 kb on - strand, within Psyr_2353 at 2728.992 kb on - strand, within Psyr_2353 at 2729.287 kb on + strand, within Psyr_2353 at 2729.362 kb on + strand, within Psyr_2353 at 2729.362 kb on + strand, within Psyr_2353 at 2729.362 kb on + strand, within Psyr_2353 at 2729.362 kb on + strand, within Psyr_2353 at 2729.362 kb on + strand, within Psyr_2353 at 2729.362 kb on + strand, within Psyr_2353 at 2729.362 kb on + strand, within Psyr_2353 at 2729.362 kb on + strand, within Psyr_2353 at 2729.363 kb on - strand, within Psyr_2353 at 2729.408 kb on + strand, within Psyr_2353 at 2729.408 kb on + strand, within Psyr_2353 at 2729.408 kb on + strand, within Psyr_2353 at 2729.409 kb on - strand, within Psyr_2353 at 2729.490 kb on - strand, within Psyr_2353 at 2729.490 kb on - strand, within Psyr_2353 at 2729.557 kb on + strand, within Psyr_2353 at 2729.558 kb on - strand, within Psyr_2353 at 2729.558 kb on - strand, within Psyr_2353 at 2729.615 kb on + strand, within Psyr_2353 at 2729.615 kb on + strand, within Psyr_2353 at 2729.615 kb on + strand, within Psyr_2353 at 2729.616 kb on - strand, within Psyr_2353 at 2729.674 kb on - strand, within Psyr_2353 at 2729.763 kb on - strand at 2729.796 kb on + strand at 2729.797 kb on - strand at 2729.800 kb on + strand at 2729.800 kb on + strand at 2729.800 kb on + strand at 2729.801 kb on - strand at 2729.801 kb on - strand at 2729.801 kb on - strand at 2729.801 kb on - strand at 2729.801 kb on - strand at 2729.801 kb on - strand at 2729.801 kb on - strand at 2729.801 kb on - strand at 2729.841 kb on + strand at 2729.841 kb on + strand at 2729.904 kb on + strand at 2729.951 kb on - strand at 2729.962 kb on + strand at 2730.308 kb on + strand, within Psyr_2354 at 2730.308 kb on + strand, within Psyr_2354 at 2730.318 kb on - strand, within Psyr_2354 at 2730.318 kb on - strand, within Psyr_2354 at 2730.350 kb on + strand, within Psyr_2354 at 2730.350 kb on + strand, within Psyr_2354 at 2730.351 kb on - strand, within Psyr_2354 at 2730.391 kb on - strand, within Psyr_2354 at 2730.395 kb on + strand, within Psyr_2354 at 2730.396 kb on - strand, within Psyr_2354 at 2730.398 kb on + strand, within Psyr_2354 at 2730.398 kb on + strand, within Psyr_2354 at 2730.399 kb on - strand, within Psyr_2354 at 2730.602 kb on + strand, within Psyr_2354 at 2730.603 kb on - strand, within Psyr_2354 at 2730.674 kb on + strand at 2730.674 kb on + strand at 2730.675 kb on - strand at 2730.707 kb on - strand at 2730.707 kb on - strand at 2730.777 kb on + strand at 2730.778 kb on - strand at 2730.778 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 2,727,631 + -1.2 2,727,631 + -0.1 2,727,632 - -2.7 2,727,632 - +0.9 2,727,632 - -0.6 2,727,702 + -0.4 2,727,743 + -0.4 2,727,926 + +0.7 2,727,926 + +0.6 2,727,949 + -0.2 2,727,950 - +1.0 2,728,003 + Psyr_2352 0.10 +0.3 2,728,075 + Psyr_2352 0.24 +0.7 2,728,075 + Psyr_2352 0.24 +0.0 2,728,075 + Psyr_2352 0.24 +1.4 2,728,075 + Psyr_2352 0.24 -0.4 2,728,076 - Psyr_2352 0.24 +2.5 2,728,076 - Psyr_2352 0.24 -0.1 2,728,225 + Psyr_2352 0.52 +0.0 2,728,225 + Psyr_2352 0.52 -0.8 2,728,378 + Psyr_2352 0.81 -1.5 2,728,378 + Psyr_2352 0.81 +0.2 2,728,378 + Psyr_2352 0.81 +0.8 2,728,378 + Psyr_2352 0.81 -2.8 2,728,379 - Psyr_2352 0.82 -2.2 2,728,379 - Psyr_2352 0.82 +0.9 2,728,379 - Psyr_2352 0.82 -0.4 2,728,379 - Psyr_2352 0.82 -2.1 2,728,475 + +0.8 2,728,475 + +0.2 2,728,495 + +0.4 2,728,495 + +0.3 2,728,495 + +1.0 2,728,496 - +0.3 2,728,530 + -2.4 2,728,530 + -0.3 2,728,531 - +0.3 2,728,531 - -0.2 2,728,531 - -0.4 2,728,565 + -0.4 2,728,565 + -0.9 2,728,565 + -1.2 2,728,565 + -0.3 2,728,566 - -1.3 2,728,566 - +0.5 2,728,566 - +0.7 2,728,566 - +0.2 2,728,566 - +0.3 2,728,570 + +0.6 2,728,571 - +1.8 2,728,571 - +0.6 2,728,571 - +1.6 2,728,574 + -0.9 2,728,575 - -0.2 2,728,934 + Psyr_2353 0.28 -0.7 2,728,987 + Psyr_2353 0.32 +0.2 2,728,987 + Psyr_2353 0.32 +1.0 2,728,988 - Psyr_2353 0.32 -1.2 2,728,988 - Psyr_2353 0.32 +1.3 2,728,991 + Psyr_2353 0.33 +2.2 2,728,991 + Psyr_2353 0.33 +1.3 2,728,991 + Psyr_2353 0.33 -1.6 2,728,991 + Psyr_2353 0.33 +0.0 2,728,991 + Psyr_2353 0.33 -1.3 2,728,992 - Psyr_2353 0.33 -1.8 2,728,992 - Psyr_2353 0.33 -2.6 2,728,992 - Psyr_2353 0.33 -0.2 2,728,992 - Psyr_2353 0.33 +1.0 2,728,992 - Psyr_2353 0.33 -1.4 2,728,992 - Psyr_2353 0.33 -0.6 2,729,287 + Psyr_2353 0.57 -0.6 2,729,362 + Psyr_2353 0.63 -2.3 2,729,362 + Psyr_2353 0.63 -0.5 2,729,362 + Psyr_2353 0.63 -0.8 2,729,362 + Psyr_2353 0.63 +0.4 2,729,362 + Psyr_2353 0.63 +0.2 2,729,362 + Psyr_2353 0.63 -0.9 2,729,362 + Psyr_2353 0.63 +0.2 2,729,362 + Psyr_2353 0.63 +1.6 2,729,363 - Psyr_2353 0.63 +0.4 2,729,408 + Psyr_2353 0.67 +0.5 2,729,408 + Psyr_2353 0.67 +0.6 2,729,408 + Psyr_2353 0.67 -2.0 2,729,409 - Psyr_2353 0.67 -0.7 2,729,490 - Psyr_2353 0.73 +2.7 2,729,490 - Psyr_2353 0.73 -0.2 2,729,557 + Psyr_2353 0.79 +0.6 2,729,558 - Psyr_2353 0.79 -2.5 2,729,558 - Psyr_2353 0.79 -0.3 2,729,615 + Psyr_2353 0.83 +0.0 2,729,615 + Psyr_2353 0.83 +0.7 2,729,615 + Psyr_2353 0.83 -2.1 2,729,616 - Psyr_2353 0.84 -1.7 2,729,674 - Psyr_2353 0.88 -2.9 2,729,763 - +1.0 2,729,796 + -1.1 2,729,797 - -1.0 2,729,800 + +0.5 2,729,800 + -0.8 2,729,800 + -0.2 2,729,801 - +1.2 2,729,801 - -2.0 2,729,801 - -2.4 2,729,801 - -0.1 2,729,801 - -0.1 2,729,801 - +0.5 2,729,801 - -1.2 2,729,801 - -2.9 2,729,841 + -1.7 2,729,841 + -0.1 2,729,904 + +0.2 2,729,951 - +1.4 2,729,962 + +1.6 2,730,308 + Psyr_2354 0.49 -2.8 2,730,308 + Psyr_2354 0.49 -0.0 2,730,318 - Psyr_2354 0.50 +1.9 2,730,318 - Psyr_2354 0.50 -0.0 2,730,350 + Psyr_2354 0.54 -2.7 2,730,350 + Psyr_2354 0.54 -0.1 2,730,351 - Psyr_2354 0.54 -0.5 2,730,391 - Psyr_2354 0.58 -0.7 2,730,395 + Psyr_2354 0.59 -0.2 2,730,396 - Psyr_2354 0.59 -0.4 2,730,398 + Psyr_2354 0.59 +0.6 2,730,398 + Psyr_2354 0.59 -0.4 2,730,399 - Psyr_2354 0.59 -0.8 2,730,602 + Psyr_2354 0.84 -0.7 2,730,603 - Psyr_2354 0.84 -1.6 2,730,674 + -0.2 2,730,674 + -1.4 2,730,675 - +0.7 2,730,707 - -1.3 2,730,707 - +1.5 2,730,777 + -0.8 2,730,778 - +1.2 2,730,778 - -1.2
Or see this region's nucleotide sequence